Effect of Chitosan Gel on Wound Healing: Experimental Study in Donkeys

Abstract

Key words:Chitosan gel, Donkeys, Histopathological changes, Wound healing Chitosan is one of the natural bioactive materials known for its wound healing effect. Healing of equine's wounds are often delayed and complicated. This study aimed to evaluate efficacy of chitosan gel on healing of equine skin-wounds in comparison to povidone-iodine (as control). Six adult male donkeys were used and divided equally into two groups; group A (2x2 cm skin-wound) and group B (4x4 cm skin-wound). Full thickness skin-wounds were created on forearms and shoulders. Wounds at the right side were irrigated using povidone-iodine, while wounds at the left side were dressed using chitosan gel. Clinically, chitosan treated wounds contracted faster than control wounds, and shoulder-wounds contracted faster than that of the forearm. By the end of the experiment all chitosan treated wounds were completely healed with intact epidermis. In contrast, 10.4 % and 13.3 % of wound's area treated with povidone-iodine in group B at the shoulder and the forearm regions, respectively, still unclosed. Grossly, povidoneiodine treated wounds in both regions, were covered with scab in group A, and had an area of granulation tissue in the wound-centers in group B. Chitosan treated wounds, in both groups in both regions, were completely covered with cornified epithelium. Microscopically, the re-epithelialization was uncompleted in povidone-iodine treated wounds in both groups in both regions. In contrary, complete epidermal layer was observed in wounds treated with chitosan in both groups in both regions. Finally, chitosan gel may be better than povidone-iodine in healing of equine skin wounds.

A Prospective Multicenter Randomized Controlled Trial to Evaluate the Efficacy of Chitosan Hydrogel Paste in Comparison to Commercial Hydroactive Gel as a Wound Bed Preparation

“…The use of a CDHP dressing on full-thickness wounds with cavities was supported by previous studies. 2 10 28 29 30 31 This dressing can keep the wound moist and enhance granulation tissue. This clinical trial demonstrated the effectiveness of CDHP dressings in wound bed preparation concerning granulation and removal of necrotic tissue from cavitation wounds.…”

Synthesis and characterization of chitosan for medical applications: A review

“…40 The Chitin demineralization process from biomass (chicken feet) was carried out by Morganti et al, 41 using 1: 18 (w/v) at 1.5 M solutions of Hcl for 36 h at room temperature. 42 While it claimed that 97% demineralization was achieved, another report by Cao, 40 stated that the demineralization efficiency was 99% when a 0.55 M solution of Hcl was used in the ratio of 1:10 (w/v) to dissolve crab shells in 15 min at room temperature. In the same study, it was found that substituting crab shells with cuttlefish shells resulted in a demineralization value of 98%, with all other parameters and concentrations unchanged.…”

Evaluation of the Efficacy of Microvesicles Derived from Mesenchymal Stem Cells on ‎Healing of Experimentally Induced Full-Thickness Skin Wound in Donkeys

“…The width and the length of wounds were measured using digital caliber after the wound had been carefully cleaned by saline to visualize wound margins. The wound surface area, percentage of wound size, and wound contraction percent were calculated using the following equations (Zaid et al, 2017).…”