Electrospun Fibers as a Dressing Material for Drug and Biological Agent Delivery in Wound Healing Applications

Asadi

J of Applied Polymer Sci

2018

Bioactive wound dressings from poly(vinyl alcohol) (PVA) and zein nanoparticles (NPs) loaded with diclofenac (DLF) were prepared successfully by the single jet electrospinning method. DLF‐loaded zein NPs with an average diameter of ∼228 nm were prepared using anti‐solvent precipitation method. The formulation of zein:DLF 1:1 exhibited optimum encapsulation efficiency of 47.80%. The NPs were characterized by dynamic light scattering, zeta‐potential measurement, and differential scanning calorimetry. In vitro, drug release profiles of the DLF‐loaded zein NPs, and PVA–zein NPs were also studied within 120 h and showed the release efficiency of nearly 80% from zein NPs. A more controlled release of DLF was achieved by embedding the zein NPs in the PVA nanofibers. Fourier transform infrared spectroscopy was used to analyze possible interactions between different components of the fabricated dressings. The mechanical properties of the developed dressings were also evaluated using uniaxial tensile testing. Young's modulus (E) of the dressings decreased after inclusion of zein NPs within the PVA nanofibers. Moreover, fibroblast culturing experiments proved that the composite dressings supported better cell attachment and proliferation compared to PVA nanofibers, by exhibiting moderate hydrophilicity. The results suggested that the electrospun composite dressing of PVA nanofibers and zein NPs is a promising topical drug‐delivery system and have a great potential for wound healing application. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46643.

Section: Introductionmentioning

confidence: 99%

Zein nanoparticle‐embedded electrospun PVA nanofibers as wound dressing for topical delivery of anti‐inflammatory diclofenac

Asadi

J of Applied Polymer Sci

2018

Stem Cells International

“…Scaffolds, hydrogel, or other biomaterials have been used as vehicles by providing controlled release of therapeutic agents and tunable release of bioactive molecules of skin engineering [115]. A study has shown that electrospun fibers can be bioactive molecule carriers for wound healing [116]. And a wide variety of bioactive molecules has been incorporated into electrospun fibers and delivered into injured sites.…”

Section: Perspectivesmentioning

confidence: 99%

Bioactive Molecules for Skin Repair and Regeneration: Progress and Perspectives

Chen

Hou

Zhong

et al. 2019

Skin regeneration is a vexing problem in the field of regenerative medicine. A bioactive molecule-based strategy has been frequently used in skin wound healing in recent years. Bioactive molecules are practical tools for regulating cellular processes and have been applied to control cellular differentiation, dedifferentiation, and reprogramming. In this review, we focus on recent progress in the use of bioactive molecules in skin regenerative medicine, by which desired cell types can be generated in vitro for cell therapy and conventional therapeutics can be developed to repair and regenerate skin in vivo through activation of the endogenous repairing potential. We further prospect that the bioactive molecule-base method might be one of the promising strategies to achieve in situ skin regeneration in the future.

“…The use of gelatin nanofibers having enough tensile strength for fabricating nonwoven mats has received attention recently for use for antimicrobial applications [45][46][47][48][49]. Successful spinning of minimum diameter nanofibers results in the surface areas of these nanofibers being increased in addition to their light weight.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning process parameters optimization for biofunctional curcumin/gelatin nanofibers

Kanu

Gupta

Vates

et al. 2020

Mater. Res. Express

Electrospinning has received wide attention for the preparation of uniform diameter nanofibers (ranging from 5 nm to several hundred nanometers) in films with random as well as aligned fashions of the fibers of various materials for use in biomedical applications. Electrospinning research has provided an in-depth understanding of the preparation of light weight, ultrathin, porous, biofunctional curcumin/gelatin nanofibers having applications in wound dressing, drug release, tissue engineering, etc. In the first half of this article, prior research on electrospun curcumin/gelatin nanofibers is reviewed in depth with nanofibers being desired due to their low diameters since these would have then large surface area to volume ratio and enough film porosity as well as improved mechanical (tensile) strength so that when prepared as mats these nanofibers (having high biocompatibility) could be used for sustained release of curcumin and oxygen to wounds during healing. The synthesis of ultrathin nanofibers (having minimum average diameter) is not a simple task unless numerical investigation is carefully done in the first half of this research article. The authors research described here examined the effects of critical process parameters (in the second half of the paper) such as distance between the spinneret and collector, flow rate, voltage and solution viscosity, on the preparation of uniform and ultrathin nanofibers using scanning electron microscopy (SEM) for characterization of the nanofibers. A 2 k factorial design of experiment was found to be a suitable and efficient technique to optimize the critical process parameters used in the preparation of the biofunctional nanofibers with the purpose of having applications in the treatment of problematic wounds such as diabetic chronic ulcers. After parametric investigation, the distance, flow rate and voltage when taken together, were found to have the most significant contributions to the preparation of minimum diameter nanofibers. The primary objective of this research was fulfilled with the development of ultrathin curcumin/gelatin nanofibers having a 181 nm (181±66 nm) average diameter using the optimized setting of a solution having 1.5% gelatin, and 1% curcumin in 10 ml of 98% concentrated formic acid, with the electrospining unit having a voltage of 10 KV, distance from the spinneret to collector drum of 15 cm, flow rate of 0.1 ml h −1 , viscosity of 65 cP and drum collector speed of 1000 rpm. However, the lowest average diameter of nanofiber was measured around 147 nm (147±34 nm) which was prepared at a higher voltage, such as 15 KV (at 10 cm distance, 0.15 ml h −1 flow rate and 65 cP viscosity) using the solution. The design of this research paper is based on the view that merely optimization of biofunctional nanofibers may not fully satisfy researchers/ engineers unless they are also provided with sufficient information about (a) the entire electrospinning mechanism (numerical investigations of the mechanism) to have better control over preparation of ultr...