Electrospun nanofibers in cancer research: from engineering of<i>in vitro</i>3D cancer models to therapy

Cavo, Marta; Serio, Francesca; Kale, Narendra; D’Amone, Eliana; Gigli, Giuseppe; Mercato, Loretta L. del

doi:10.1039/d0bm00390e

Cited by 67 publications

(93 citation statements)

References 172 publications

(180 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This process was first used in the field of tissue engineering but meanwhile it is also applied to engineer 3D cancer models. These fibrous materials are able to mimic the ECM of living tissue ( Cavo et al, 2020 ). The use of different polymers enables the possibility to adapt the properties of the scaffold to the varying properties of ECMs from different tumor entities.…”

Section: Electrospun Nanofibresmentioning

confidence: 99%

See 1 more Smart Citation

Precision Medicine Gains Momentum: Novel 3D Models and Stem Cell-Based Approaches in Head and Neck Cancer

Affolter

Lammert

Kern

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Despite the current progress in the development of new concepts of precision medicine for head and neck squamous cell carcinoma (HNSCC), in particular targeted therapies and immune checkpoint inhibition (CPI), overall survival rates have not improved during the last decades. This is, on the one hand, caused by the fact that a significant number of patients presents with late stage disease at the time of diagnosis, on the other hand HNSCC frequently develop therapeutic resistance. Distinct intratumoral and intertumoral heterogeneity is one of the strongest features in HNSCC and has hindered both the identification of specific biomarkers and the establishment of targeted therapies for this disease so far. To date, there is a paucity of reliable preclinical models, particularly those that can predict responses to immune CPI, as these models require an intact tumor microenvironment (TME). The “ideal” preclinical cancer model is supposed to take both the TME as well as tumor heterogeneity into account. Although HNSCC patients are frequently studied in clinical trials, there is a lack of reliable prognostic biomarkers allowing a better stratification of individuals who might benefit from new concepts of targeted or immunotherapeutic strategies. Emerging evidence indicates that cancer stem cells (CSCs) are highly tumorigenic. Through the process of stemness, epithelial cells acquire an invasive phenotype contributing to metastasis and recurrence. Specific markers for CSC such as CD133 and CD44 expression and ALDH activity help to identify CSC in HNSCC. For the majority of patients, allocation of treatment regimens is simply based on histological diagnosis and on tumor location and disease staging (clinical risk assessments) rather than on specific or individual tumor biology. Hence there is an urgent need for tools to stratify HNSCC patients and pave the way for personalized therapeutic options. This work reviews the current literature on novel approaches in implementing three-dimensional (3D) HNSCC in vitro and in vivo tumor models in the clinical daily routine. Stem-cell based assays will be particularly discussed. Those models are highly anticipated to serve as a preclinical prediction platform for the evaluation of stable biomarkers and for therapeutic efficacy testing.

show abstract

Section: Electrospun Nanofibresmentioning

confidence: 99%

“…Such fibrous scaffolds were developed for many tumor types like breast cancer, pancreatic cancer and many more. A comprehensive summary of tumor types for which such models exist can be found in Cavo’s review ( Cavo et al, 2020 ). However, such models are currently not published for HNC.…”

Section: Electrospun Nanofibresmentioning

confidence: 99%

Precision Medicine Gains Momentum: Novel 3D Models and Stem Cell-Based Approaches in Head and Neck Cancer

Affolter

Lammert

Kern

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Currently, nanofibrous polymer materials are being thoroughly investigated for a variety of medical uses. The use of nanofiber system drug carriers in anticancer therapy offers numerous benefits, including the ability to form fibers with varying diameters ranging from nanometers to sub-microns, surface modification, having a large outer surface ratio and unique porosity to maximize the drug encapsulation process, controlled and sustained drug release in the desired workplace to improve efficacy [86][87][88][89][90][91].…”

Section: Nanofibersmentioning

confidence: 99%

Nanoformulations of α-Mangostin for Cancer Drug Delivery System

et al. 2021

View full text Add to dashboard Cite

Natural compounds are emerging as effective agents for the treatment of malignant diseases. The active constituent of α-mangostin from the pericarp of Garcinia mangostana L. has earned significant interest as a plant base compound with anticancer properties. Despite α-mangostin’s superior properties as an anticancer agent, its applications are limited due to its poor solubility and physicochemical stability, rapid systemic clearance, and low cellular uptake. Our review aimed to summarize and discuss the nanoparticle formulations of α-mangostin for cancer drug delivery systems from published papers recorded in Scopus, PubMed, and Google Scholar. We investigated various types of α-mangostin nanoformulations to improve its anticancer efficacy by improving bioavailability, cellular uptake, and localization to specific areas These nanoformulations include nanofibers, lipid carrier nanostructures, solid lipid nanoparticles, polymeric nanoparticles, nanomicelles, liposomes, and gold nanoparticles. Notably, polymeric nanoparticles and nanomicelles can increase the accumulation of α-mangostin into tumors and inhibit tumor growth in vivo. In addition, polymeric nanoparticles with the addition of target ligands can increase the cellular uptake of α-mangostin. In conclusion, nanoformulations of α-mangostin are a promising tool to enhance the cellular uptake, accumulation in cancer cells, and the efficacy of α-mangostin as a candidate for anticancer drugs.

show abstract

“…Biocompatible polymer fibers with nano‐ and micro‐scale sizes have been widely used in various tissue engineering and biomedical applications, such as drug delivery, wound healing, and in vitro modeling 14–18 . The fibrous membranes show substantial surface‐to‐volume ratio and interconnected structures, making them closely mimic the structural architecture of the native extracellular matrix (ECM) 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Biocompatible polymer fibers with nano-and micro-scale sizes have been widely used in various tissue engineering and biomedical applications, such as drug delivery, wound healing, and in vitro modeling. [14][15][16][17][18] The fibrous membranes show substantial surface-tovolume ratio and interconnected structures, making them closely mimic the structural architecture of the native extracellular matrix (ECM). 19 If necessary, the fibers can be further loaded with bioactive substances such as proteins, peptides, DNAs, RNAs, and smallmolecule drugs, to modulate cell behavior.…”

mentioning

confidence: 99%

Airbrushed nanofibrous membranes to control stem cell infiltration in 3D‐printed scaffolds

et al. 2021

View full text Add to dashboard Cite

Extrusion‐based 3D printing of polymeric biomaterials has emerged as a promising approach for the fabrication of complex tissue engineering constructs. However, the large pore and feature size lead to low cell seeding efficiency and limited control of spatial distribution of cells within the scaffolds. We developed hybrid scaffolds that are composed of 3D‐printed layers and airbrushed fibrous membranes. Airbrushing time was adjusted to fabricate low (L), medium (M), and high (H) density membranes to effectively control stem cell infiltration. When two distinct populations of stem cells were seeded from top or bottom of the scaffolds, scaffolds composed of LLL membranes showed gradual mixing of the cells with depth, whereas LHL membranes led to two distinct regions of cells separated by the H membrane. Our results demonstrate that fibrous membranes incorporated within 3D‐printed layers enable user‐defined and spatially controlled cell compositions within hybrid scaffolds.

show abstract

Electrospun nanofibers in cancer research: from engineering ofin vitro3D cancer models to therapy

Abstract: This review provides a comprehensive summary of the recent advances of electrospun biomaterials in the field of cancer research and highlights their future prospects.

Cited by 67 publications

References 172 publications

Precision Medicine Gains Momentum: Novel 3D Models and Stem Cell-Based Approaches in Head and Neck Cancer

Precision Medicine Gains Momentum: Novel 3D Models and Stem Cell-Based Approaches in Head and Neck Cancer

Nanoformulations of α-Mangostin for Cancer Drug Delivery System

Airbrushed nanofibrous membranes to control stem cell infiltration in 3D‐printed scaffolds

Contact Info

Product

Resources

About