Electrospun Nanofibrous Membranes: A Novel Solid Substrate for Microfluidic Immunoassays for HIV

Yang, Dayong; Niu, Xiamu; Liu, Yingyi; Wang, Yan; Gu, Xuan; Song, Lusheng; Zhao, Rui; Ma, Liying; Shao, Yijie; Jiang, Xingyu

doi:10.1002/adma.200801302

Cited by 151 publications

(118 citation statements)

References 37 publications

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“…[60] We prepared a uniform PC fibers through ES method and then convert fibers into stable and flat membrane using a wet-press method for proteins immobilizing (Figure 8a,b). [61] This porous fiber membrane can better promote the adsorption of IgG and lower the LOD compared to conventional types of membranes (Figure 8c,d). PVDF porous membrane are made using the same method (Figure 8e).…”

Section: Materials For Enhanced Immunoassaymentioning

confidence: 99%

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Materials for Microfluidic Immunoassays: A Review

Mou

Jiang

2017

Adv Healthcare Materials

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120

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Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user‐friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials‐related aspects of the recent advances in microfluidics‐based immunoassays for point‐of‐care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.

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Section: Materials For Enhanced Immunoassaymentioning

confidence: 99%

“…Reproduced with permission. [61] e) PVDF porous membrane used in cross-array assay for signal enhancement. Reproduced with permission.…”

Section: Poc Diagnosismentioning

confidence: 99%

Materials for Microfluidic Immunoassays: A Review

Mou

Jiang

2017

Adv Healthcare Materials

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120

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“…The examples include HIV detection, 71 histidine-tagged protein purification, 72 and cross-array protein immunoassays 73 and are aimed for biosensing applications. However, none of these approaches offer any precise spatial or hierarchical control of the deposition of electrospun fibers, nor the possibility to vary crucial fiber parameters such as alignment orientation and diameter in the same device.…”

Section: Bonding Of Microfluidic Channels Over Patterned Fibersmentioning

confidence: 99%

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

Wallin

Zandén²,

Carlberg³

et al. 2012

Biomicrofluidics

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The properties of a cell's microenvironment are one of the main driving forces in cellular fate processes and phenotype expression in vivo. The ability to create controlled cell microenvironments in vitro becomes increasingly important for studying or controlling phenotype expression in tissue engineering and drug discovery applications. This includes the capability to modify material surface properties within well-defined liquid environments in cell culture systems. One successful approach to mimic extra cellular matrix is with porous electrospun polymer fiber scaffolds, while microfluidic networks have been shown to efficiently generate spatially and temporally defined liquid microenvironments. Here, a method to integrate electrospun fibers with microfluidic networks was developed in order to form complex cell microenvironments with the capability to vary relevant parameters. Spatially defined regions of electrospun fibers of both aligned and random orientation were patterned on glass substrates that were irreversibly bonded to microfluidic networks produced in poly-dimethyl-siloxane. Concentration gradients obtained in the fiber containing channels were characterized experimentally and compared with values obtained by computational fluid dynamic simulations. Velocity and shear stress profiles, as well as vortex formation, were calculated to evaluate the influence of fiber pads on fluidic properties. The suitability of the system to support cell attachment and growth was demonstrated with a fibroblast cell line. The potential of the platform was further verified by a functional investigation of neural stem cell alignment in response to orientation of electrospun fibers versus a microfluidic generated chemoattractant gradient of stromal cell-derived factor 1 alpha. The described method is a competitive strategy to create complex microenvironments in vitro that allow detailed studies on the interplay of topography, substrate surface properties, and soluble microenvironment on cellular fate processes. V C 2012 American Institute of Physics. [http://dx

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“…We focused on major diseases related to human health such as AIDS caused by HIV. 23 Microfluidic devices provide a powerful tool for high-throughput biomedical diagnosis. In this study, we tested 30 human sera simultaneously, and analyzed the result using the developed program.…”

Section: High-throughput Hiv Detectionmentioning

confidence: 99%

Matrix-localization for fast analysis of arrayed microfluidic immunoassays

et al. 2012

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High-throughput assays necessitate high-throughput data analysis. Arrayed microfluidic immunoassay shows the capability of high-throughput protein detection. However, its development was restricted by the low efficiency of downstream data analysis. We present herein programming-based image processing through the local recognition of a sub-array followed by the region-growing algorithm to achieve fast, convenient and precise extraction of information with reduced personal bias.

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Electrospun Nanofibrous Membranes: A Novel Solid Substrate for Microfluidic Immunoassays for HIV

Cited by 151 publications

References 37 publications

Materials for Microfluidic Immunoassays: A Review

Materials for Microfluidic Immunoassays: A Review

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

Matrix-localization for fast analysis of arrayed microfluidic immunoassays

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