Electrospun PCL nanofibers blended with Wattakaka volubilis active phytochemicals for bone and cartilage tissue engineering

Venugopal, Elakkiya; Sahanand, K. Santosh; Bhattacharyya, Amitava; Selvakumar, R.

doi:10.1016/j.nano.2019.102044

Cited by 33 publications

(15 citation statements)

References 20 publications

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“…Synthetic polymers have good plasticity, and their microstructure, morphology, and degradation rate can be predesigned and regulated according to the biology of specific tissue ( Wang et al, 2020b ). PCL ( Venugopal et al, 2019 ), PLA ( Baena et al, 2019 ), PGA ( Lin et al, 2017 ), and PLGA ( Kim et al, 2019 ) are the most representative and widely used synthetic polymers, and have been approved by the FDA for clinical human use. Scaffolds made from synthetic polymers temporarily provide mechanical support after implantation, as well as a microenvironment for cell growth, proliferation and differentiation, thereby regulating and inducing tissue differentiation and regeneration ( Li S. et al, 2020 ; Salonius et al, 2020 ).…”

Section: Immunological Characterization Of Biomaterialsmentioning

confidence: 99%

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Liu

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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Biomaterials play a core role in cartilage repair and regeneration. The success or failure of an implanted biomaterial is largely dependent on host response following implantation. Host response has been considered to be influenced by numerous factors, such as immune components of materials, cytokines and inflammatory agents induced by implants. Both synthetic and native materials involve immune components, which are also termed as immunogenicity. Generally, the innate and adaptive immune system will be activated and various cytokines and inflammatory agents will be consequently released after biomaterials implantation, and further triggers host response to biomaterials. This will guide the constructive remolding process of damaged tissue. Therefore, biomaterial immunogenicity should be given more attention. Further understanding the specific biological mechanisms of host response to biomaterials and the effects of the host-biomaterial interaction may be beneficial to promote cartilage repair and regeneration. In this review, we summarized the characteristics of the host response to implants and the immunomodulatory properties of varied biomaterial. We hope this review will provide scientists with inspiration in cartilage regeneration by controlling immune components of biomaterials and modulating the immune system.

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Section: Immunological Characterization Of Biomaterialsmentioning

confidence: 99%

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Liu

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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“…Various biodegradable polymers including poly(lactide) (PLA) [5], poly (glycolic acid) (PGA) [6], poly-L-lactide (PLLA) [7], poly(ε-caprolactone) (PCL) [5,8,9], poly (lactic-co-glycolic acid) (PLGA) [10], poly(ethylene glycol) (PEG) [11,12], poly(hydroxybutyrate) (PHB) [6,13], and its copolymer poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) [9,14] are frequently used to fabricate into scaffolds for tissue engineering. The thermogel of stereocomplex of PLA and PEG modified with cholesterol were fabricated as cartilage scaffolds which exhibited high mechanical strength and preserved gene expression of chondrocytes [11].…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Kosorn

Wutticharoenmongkol

2021

International Journal of Polymer Science

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The scaffolds of poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PCL/PHBV) blends were fabricated from fused deposition modeling. From indirect cytotoxicity testing based on mouse fibroblasts, all scaffolds with various blend ratios were nontoxic to cells. The surface-treated scaffold with a blend ratio of 25/75 PCL/PHBV exhibited the highest proliferation of porcine chondrocytes and total glycosaminoglycans (GAGs) after 21 days of culture. The scaffolds with a blend ratio of 25/75 with local pores (LP) were prepared from FDM along with a salt leaching technique using NaCl as porogens. The effect of NaOH in surface treatment on the biological property of scaffolds was investigated. The scaffolds with LP and with 1 M NaOH surface treatment exhibited the highest proliferation of cells and total GAGs after 28 days of culture. The degradation behaviors of the scaffolds were studied. The nonsurface treated, surface treated without LP, and surface treated with LP scaffolds were degraded in phosphate buffer (pH 7.4) for 30 days at 37°C and 50°C for nonenzymatic condition and at 37°C for enzymatic condition. The surface treated with LP scaffold showed the highest amount of weight loss, followed by the surface treated without LP, and the nonsurface-treated scaffolds without LP, respectively. The results from Fourier-transform infrared spectroscopy indicated degradation of PCL and PHBV through hydrolysis of the ester functional group. The compressive strengths of all scaffolds were sufficiently high. The results suggested that the scaffolds with the existence of LP and with surface treatment showed the highest potential for use as cartilage scaffolds.

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“…When the saturated scaffold was removed from the cylinder, the residue ethanol volume was recorded as v3. Porosity = (v1 – v3)/(v2 – v3) ×100% . All experiments were repeated for three times.…”

Section: Methodsmentioning

confidence: 99%

Effects of Fiber Cross-Angle Structures on the Mechanical Property of 3D Printed Scaffolds and Performance of Seeded MC3T3-E1 Cells

et al. 2021

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The three-dimensional (3D) printing technology combined with bone tissue engineering has become one of the major methods for mandibular reconstruction. However, the key factor retarding mandible reconstruction is the barrier of understanding and achieving the complex 3D gridwork formed by the trabeculae. This study innovatively constructed a low-temperature 3D printing silk fibroin/collagen/hydroxyapatite (SF/COL/HA) composite scaffold with a stable structure and remarkable biocompatibility. We designed three kinds of six-layer scaffolds with mixed fiber cross-angle structures (FCAS) of [0°/90°/0°/90°/0°/90°], [0°/45°/90°/135°/180°/225°] and [0°/30°/60°/90°/120°/150°]. Material properties of these scaffolds such as porosity, water absorption rate, X-ray diffraction, Fourier transform infrared spectroscopy, and compression performance were detected. Then, the MC3T3-E1 cells were seeded on these scaffolds and the adhesion, proliferation, and differentiation were investigated. To be more convincing, the same experiments were performed on another polycaprolactone/hydroxyapatite scaffold. The results suggested that the changes of FCAS affected the mechanical properties of 3D printed scaffolds and performance of seeded cells. Besides, the 90° FCAS significantly enhanced the compressive modulus in two groups and were more conducive to the cell proliferation and osteogenesis, which provided evidence for exploring the influence of FCAS on the properties of scaffolds and the application of two composite scaffolds in tissue regeneration.

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Electrospun PCL nanofibers blended with Wattakaka volubilis active phytochemicals for bone and cartilage tissue engineering

Cited by 33 publications

References 20 publications

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Effects of Fiber Cross-Angle Structures on the Mechanical Property of 3D Printed Scaffolds and Performance of Seeded MC3T3-E1 Cells

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