Electrospun Polyhydroxybutyrate and Poly(L-lactide-co-ε-caprolactone) Composites as Nanofibrous Scaffolds

Abstract

Electrospinning can produce nanofibrous scaffolds that mimic the architecture of the extracellular matrix and support cell attachment for tissue engineering applications. In this study, fibrous membranes of polyhydroxybutyrate (PHB) with various loadings of poly(L-lactide-co-ε-caprolactone) (PLCL) were successfully prepared by electrospinning. In comparison to PLCL scaffolds, PLCL blends with PHB exhibited more irregular fibre diameter distributions and higher average fibre diameters but there were no significant differences in pore size. PLCL/PHB scaffolds were more hydrophilic (<120°) with significantly reduced tensile strength (ca. 1 MPa) compared to PLCL scaffolds (150.9 ± 2.8° and 5.8 ± 0.5 MPa). Increasing PLCL loading in PHB/PLCL scaffolds significantly increased the extension at break, (4–6-fold). PLCL/PHB scaffolds supported greater adhesion and proliferation of olfactory ensheathing cells (OECs) than those exhibiting asynchronous growth on culture plates. Mitochondrial activity of cells cultivated on the electrospun blended membranes was enhanced compared to those grown on PLCL and PHB scaffolds (212, 179, and 153%, resp.). Analysis showed that PLCL/PHB nanofibrous membranes promoted cell cycle progression and reduced the onset of necrosis. Thus, electrospun PLCL/PHB composites promoted adhesion and proliferation of OECs when compared to their individual PLCL and PHB components suggesting potential in the repair and engineering of nerve tissue.

Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices

“…The relative population of OECs in each phase of the cell division cycle are presented in Figures 4 , 6 . Samples were compared to TCP controls, a substrate on which the health of OECs has been investigated in prior studies (Daranarong et al, 2014 ). These controls were established by placing the cells in environmental conditions that have been shown to arrest cell development at a particular stage of the cell cycle.…”

“…The impact of biphasic stimulation on OEC viability, apoptosis and cell cycle was assessed by image-based cytometry as described by Daranarong et al ( 2014 ). Briefly, the harvested cell samples were centrifuged (700 rpm, 5 min) and rinsed in DPBS.…”

Chitosan as a Biomaterial: Influence of Degree of Deacetylation on Its Physiochemical, Material and Biological Properties

“…Controls for DNA content were simultaneously conducted including: (1) cells in the absence of chitosan films, (2) cells in serum-free medium, and cells synchronised (48 h) in medium containing either 1μg mL -1 aphidicolin (3) or 2 μM nocodazole (4). Similar controls, but with the absence of (3) aphidicolin, were used in the determination of apoptotic indices associated with membrane externalisation of phosphotidylserine (PS) [ 29 , 30 ].…”

Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nano- bioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

“…Furthermore, alloy fibers showed higher tensile strength compared with pure PHB and PHBV fibers and were also suitable substrate for the proliferation of human osteoblast cells and L929 mouse fibroblasts. In another study,[ 33 ] Daranarvng et al . constructed the composite scaffold of PHB/poly(L-lactide-co-ε-caprolactone) (PCL) by electrospinning that had the proper hydrophilic feature and high tensile strength compared to the PHB scaffolds.…”