Electrospun protein fibers as matrices for tissue engineering

Li, Mengyan; Mondrinos, Mark J.; Gandhi, Milind; Ko, Frank; Weiss, Anthony S.; Lelkes, Peter I.

doi:10.1016/j.biomaterials.2005.03.030

Cited by 739 publications

(493 citation statements)

References 45 publications

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“…Exposing the electrospun scaffold to glutaraldehyde intermolecularly cross-linked the scaffolds, making cell culturing possible; however, cross-linking reduced the porosity dramatically. Currently, only glutaraldehyde has been investigated as a cross-linking agent for electrospun collagen based structures [3,16,42,43]. However, glutaraldehyde-treated materials can be cytotoxic [44].…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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Section: Discussionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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“…Earlier investigations 2,4 have specified that highly functional proteins are the best candidates for developing electrospun nanofibers for biomedical applications because of their outstanding properties, such as water solubility, biocompatibility and biodegradability: moreover, proteins can be tailored as desired by modifying their functional groups. In addition, proteins are often readily available in abundance as byproducts of food processing and are economically viable.…”

mentioning

confidence: 99%

“…From the literature, [1][2][3][4][5][6] it is understood that very fine polymer-based nanofibers can be produced using electrospinning, which is a relatively simple and versatile technique to achieve smooth, fine nanofibers with large surface areas. Nanofibers obtained using the electrospinning technique are considered more oriented and therefore exhibit better mechanical and thermal properties than do those obtained using other methods.…”

mentioning

confidence: 99%

Development of non-woven nanofibers of egg albumen-poly (vinyl alcohol) blends: influence of solution properties on morphology of nanofibers

Rathna

Jog

Gaikwad

2011

Polym J

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Egg albumen (EA), a highly functional globular protein with desirable properties, is the least-explored material for biomaterial applications, although it is available in abundance. In our studies, we explored the viability of EA and various blends with biocompatible and non-toxic poly (vinyl alcohol) (PVA) to produce nanofibers for biomedical applications. EA and PVA blends were prepared in various compositions. Electrospinning was used to fabricate non-woven nanofibers. Solution properties, such as viscosity and electrical conductivity, were evaluated for various prepared solutions. Solution viscosity increased with increasing polymer concentration. Solutions with higher contents of EA recorded increased conductivity, which decreased with increasing PVA content. The influence of solution properties on the morphological appearance of as-spun products was studied using scanning electron microscopy. Instead of nanofibers, nanoparticles and microparticles of EA were produced at even higher contents. In contrast, a gradual increase in the addition of PVA content to 8% EA solution resulted in the transformation of particles from large agglomerates to very fine fibers (E100 nm in diameter) because of the influence of polymer content, viscosity and conductivity. The polymer-polymer interactions in the prepared materials have been validated by Fourier transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and gel electrophoresis.

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“…Natural polymers dissolve well in 1,1,1,3,3,3-hexafluoro-2-propanol and trifluoroacetic acid [4][5][6][7], which are toxic and expensive solvents, features that limit their use in applications on an industrial scale.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning of Gelatin Functionalized with Silver Na-noparticles for Nanofiber Fabrication

Oraby¹,

Waley²,

El‐Dewany³

et al. 2013

MNSMS

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The present paper deals with gelatin nanofibres functionalized with silver nanoparticles, prepared by electrospinning using solutions of gelatin mixed with silver nitrate (AgNO 3 ). As a common solvent for gelatin and silver nitrate (AgNO 3 ), a mixture of acetic acid and water (70:30 v/v) was selected. In this system, acetic acid was used as a solvent for gelatin, and at the same time reducing agent for silver ions in solution. Silver nanoparticles (nAg) were stabilized through a mechanism that involves an interaction of the oxygen atoms of the carbonyl groups of gelatin. The viscosity and the conductivity of the gelatinous solutions were found to increase with the solution concentration. There is an observed decrease in the viscosity of the nAg containing gelatin solutions with the aging time increasing, whereas the conductivity of the AgNO 3 -containing gelatin solutions was greater than that of the base gelatin solution. The gelatin nanofibres functionalized with silver nanoparticles were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and antimicrobial test. The results of investigations by TEM and XRD confirmed the presence of silver nanoparticles with diameters in the range of (2 -10 nm), uniformly distributed over the surface of smooth nanofibres with an average diameter of 70 nm. The release of silver ions from both the 2-and 4-hrs crosslinked nAg containing gelatin fiber mats by a total immersion method in buffer and distilled water occurred rapidly during the first 60 minutes, and increased gradually afterwards. Lastly, the tests demonstrated that gelatin/Ag nanofibers have a good antimicrobial activity against some common bacteria found on burned wounds. The antibacterial activity of these materials was greatest against Staphylococcus aureus, followed by Escherichia coli, and Pseudomonas aeroginosa ≈ Candida albicans.

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Electrospun protein fibers as matrices for tissue engineering

Cited by 739 publications

References 45 publications

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Development of non-woven nanofibers of egg albumen-poly (vinyl alcohol) blends: influence of solution properties on morphology of nanofibers

Electrospinning of Gelatin Functionalized with Silver Na-noparticles for Nanofiber Fabrication

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