Electrospun scaffolds for wound healing applications from poly(4‐hydroxybutyrate): A biobased and biodegradable linear polymer with high elastomeric properties

Abstract

Electrospun scaffolds of the biodegradable and biocompatible poly‐4‐hydroxybutyrate (P4HB) polyester have been prepared using horizontal and vertical set‐up configurations of electrospinning. Specifically, it has been evaluated the influence of solvent, polymer concentration, and processing parameters, such as applied voltage, flow rate, and needle tip‐collector distance. Scaffolds obtained under the most favorable conditions were characterized in terms of crystallinity, lamellar supramolecular order, thermal (including calorimetric and thermogravimetric data), mechanical, and surface properties. Results pointed out significant differences with respect to commercial sutures (based in P4HB, e.g., MonoMax®) and demonstrated that electrospun scaffolds were constituted by crystalline microfibers with a tangled distribution that leads to high modulus Young modulus (4 MPa), maximum strength (28 MPa), and elongation (360%). Furthermore, new scaffolds had thermal stability and a rough surface that led to a hydrophobic character (105°). Scaffolds could also be successfully loaded during the electrospinning process with a peptide analog to the fibroblast growth factor (e.g., CYRSRKYSSWYVALKRC), giving rise to fully biocompatible samples with a clear acceleration in wound healing.

Absorbable Electrospun Poly-4-hydroxybutyrate Scaffolds as a Potential Solution for Pelvic Organ Prolapse Surgery

“…For the current study, we produced our own absorbable electrospun P4HB scaffolds. P4HB was chosen as knitted P4HB implants have shown promise in reconstructive surgery for ventral hernia repair and in our preclinical studies for pelvic floor repair. ,,, In addition, electrospun P4HB for other applications such as a dural or dermal , substitution are encouraging, but the available studies are limited, and no studies on electrospun P4HB for pelvic floor repair have been conducted. Because fibroblasts are the main cell type of the connective tissue of the vaginal wall and are responsible for maintaining the ECM, , their function is critical in assessing implant performance in the pelvic floor and to predict how the implant will interact with the host.…”

Medicated Scaffolds Prepared with Hydroxyapatite/Streptomycin Nanoparticles Encapsulated into Polylactide Microfibers

“…The assay controls consisted of cells not exposed to the extracts (reference for 100% cell viability), extract of nanoparticles without the STR load and a positive control based on an STR solution (10 mg/mL). Cultures were maintained for 24 h and then the cell viability was determined by the MTT assay [52]. Culture media were aspirated and cells were washed twice with PBS.…”