Electrostatic Interactions Modulate the Conformation of Collagen I

Freudenberg, Uwe; Behrens, Sven H.; Welzel, Petra B.; Müller, Martin; Grimmer, Milauscha; Salchert, Katrin; Taeger, Tilman; Schmidt, Kati; Pompe, W.; Werner, Carsten

doi:10.1529/biophysj.106.094284

Cited by 104 publications

(100 citation statements)

References 65 publications

(101 reference statements)

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“…On the other hand, KCl addition leads to substantially lower Young's moduli (Figure 6b), even though other reports have also suggested that K + promotes collagen aggregation. 31 The changes we observe with the addition of CaCl 2 and KCl contrast with the minimal effect that similar concentrations of NaCl have on stiffness (Figure 6c). …”

Section: Correlating Mechanical Stiffness With Fibril Contentmentioning

confidence: 74%

Correlating Mechanical Properties with Aggregation Processes in Electrochemically Fabricated Collagen Membranes

Poduska

2009

Biomacromolecules

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We show that mechanical stiffness is a useful metric for characterizing complex collagen assemblies, providing insight about aggregation products and pathways in collagen-based materials.This study focuses on mechanically robust collagenous membranes produced by an electrochemical synthesis process. Changing the duration of the applied electric field, or adjusting the electrolyte composition (by adding Ca 2+ , K + , Na + or by changing pH), produces membranes with a range of Young's moduli as determined from force-displacement measurements with an atomic force microscope. The structural organization -characterized by UV-visible spectroscopy, Raman spectroscopy, optical microscopy and atomic force microscopy -correlates with the mechanical stiffness. These data provide insights into the relative importance of different aggregation pathways enabled by our multi-parameter electrochemically-induced collagen assembly process.

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Section: Correlating Mechanical Stiffness With Fibril Contentmentioning

confidence: 74%

Correlating Mechanical Properties with Aggregation Processes in Electrochemically Fabricated Collagen Membranes

Poduska

2009

Biomacromolecules

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“…Adsorbing collagen to the PDMS surface by hydrophobic interactions possibly modulates the correct positioning of the glycine residues in the triple helix, thereby producing a collagen conformation that mediates only limited cell adhesion. Moreover, ionic strength has been described to modulate the collagen structure by shifting its isoelectric point [55]. Each polypeptide chain of collagen type I contains a high amount (15-20%) of ionizable residues [56], rendering electrostatic interactions important for the stability of the triple helix.…”

Section: Discussionmentioning

confidence: 99%

The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

et al. 2009

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“…For example, an increase of the ionic strength with KCl from 10 -4 to 10 -2 M shifts the isoelectric point of the collagen from pH 7.5 to 5.3. A similar increase of the ionic strength in CaCl 2 solutions shifts the pI of the same protein from 7.5 to above pH 9 (Freudenberg et al 2007). Actually, the isoelectric points of native proteins under in vivo conditions cannot be estimated on the basis of in vitro experiments or calculated on the basis of primary sequences of proteins.…”

Section: Introductionmentioning

confidence: 89%

A Possible Role of Intracellular Isoelectric Focusing in the Evolution of Eukaryotic Cells and Multicellular Organisms

Flegr

2009

J Mol Evol

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A new scenario of the origin of eukaryotic cell and multicellularity is presented. A concentric pH-gradient has been shown to exist in the cytosol of eukaryotic cells. The most probable source of such gradient is its self-formation in gradient of electric field between center and periphery of a cell. Theoretical analysis has shown that, for example, a cell of Saccharomyces cerevisiae has enough energy to continuously sustain such gradient of strength about 1.5 kV/cm, the value sufficient for effective isoelectric focusing of cytoplasmic proteins. Focusing of enzymes could highly increase the effectiveness of an otherwise diffusion-limited metabolism of large cells by concentrating enzymes into small and distinct parts of a cytoplasm. By taking away an important physical constraint to the volume of cytoplasm, the intracellular isoelectric focusing enabled evolution of cells 3-4 order of magnitude larger than typical prokaryotic cells. This opened the way for the origin of phagocytosis and lately for the development of different forms of endosymbiosis, some of them resulting in an endosymbiotic origin of mitochondria and plastids. The large volume of a cell-enabled separation of nuclear and cytoplasmic compartments which was a precondition for separation of transcription and translation processes and therefore also for the origin of various RNA-preprocessing mechanisms. The possibility to regulate gene expression by postprocessing RNA and to regulate metabolism by an electrophoretic translocation enzymes between different parts of cytoplasm by changing their isoelectric points opened the way for cell and tissue differentiation and therefore for the origin of complex multicellular organisms.

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Electrostatic Interactions Modulate the Conformation of Collagen I

Cited by 104 publications

References 65 publications

Correlating Mechanical Properties with Aggregation Processes in Electrochemically Fabricated Collagen Membranes

Correlating Mechanical Properties with Aggregation Processes in Electrochemically Fabricated Collagen Membranes

The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

A Possible Role of Intracellular Isoelectric Focusing in the Evolution of Eukaryotic Cells and Multicellular Organisms

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