Electrostatically-controlled protein adsorption onto lipid bilayer: Modeling adsorbate aggregation behavior

Trusova, Valeriya; Gorbenko, Galyna

doi:10.1016/j.bpc.2007.12.007

Cited by 8 publications

(4 citation statements)

References 57 publications

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“…Protein adsorption depends on the difference in the charge interaction and protein conformation of the scaffolds [25]. The function of a protein is determined by the protein conformation and the activity of the protein is controlled by the orientation and or its conformation [37]. The greater cell adhesion and growth observation observed on Ar scaffolds than Medpor could be due to both increased protein adsorption and more proteins were adsorbed in optimal conformations despite Ar scaffolds having a hydrophilic surface.…”

Section: Discussionmentioning

confidence: 99%

Argon plasma modified nanocomposite polyurethane scaffolds provide an alternative strategy for cartilage tissue engineering

Kalaskar

Butler

2019

J Nanobiotechnol

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Background Children born with a small or absent ear undergo surgical reconstruction to create a suitable replacement using rib cartilage. To overcome the donor site morbidity and long-term pain of harvesting rib cartilage, synthetic materials can be a useful alternative. Medpor, is the currently used synthetic polyethylene material to replace missing facial cartilage but unfortunately it has high levels of surgical complications including infection and extrusion, making it an unsuitable replacement. New materials for facial cartilage reconstruction are required to improve the outcomes of surgical reconstruction. This study has developed a new nanomaterial with argon surface modification for auricular cartilage replacement to overcome the complications with Medpor. Results Polyurethanes nanocomposites scaffolds (PU) were modified with argon plasma surface modification (Ar) and compared to Medpor in vitro and in vivo. Ar scaffolds allowed for greater protein adsorption than Medpor and PU after 48 h (p < 0.05). Cell viability and DNA assays demonstrated over 14-days greater human dermal fibroblast adhesion and cell growth on Ar than PU and Medpor nanocomposites scaffolds (p < 0.05). Gene expression using RT-qPCR of collagen-I, fibronectin, elastin, and laminin was upregulated on Ar scaffolds compared to Medpor and PU after 14-days (p < 0.05). Medpor, unmodified polyurethane and plasma modified polyurethane scaffolds were subcutaneously implanted in the dorsum of mice for 12 weeks to assess tissue integration and angiogenesis. Subcutaneous implantation of Ar scaffolds in mice dorsum, demonstrated significantly greater tissue integration by H&E and Massons trichrome staining, as well as angiogenesis by CD31 vessel immunohistochemistry staining over 12-weeks (p < 0.05). Conclusions Argon modified polyurethane nanocomposite scaffolds support cell attachment and growth, tissue integration and angiogenesis and are a promising alternative for facial cartilage replacement. This study demonstrates polyurethane nanocomposite scaffolds with argon surface modification are a promising biomaterial for cartilage tissue engineering applications. Electronic supplementary material The online version of this article (10.1186/s12951-019-0477-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Argon plasma modified nanocomposite polyurethane scaffolds provide an alternative strategy for cartilage tissue engineering

Kalaskar

Butler

2019

J Nanobiotechnol

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“…'Hard' proteins have strong internal coherence and structural rearrangements that only make small contributions to the adsorption process. These proteins prefer to be adsorbed onto hydrophobic surfaces, whereas they can also be adsorbed on hydrophilic surfaces if they are electrostatically attracted [181,182]. 'Soft' proteins, which have lower structural stability, can be adsorbed even under more rigorous conditions.…”

Section: Influencing Factors From Proteinsmentioning

confidence: 99%

A review of protein adsorption on bioceramics

et al. 2012

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Bioceramics, because of its excellent biocompatible and mechanical properties, has always been considered as the most promising materials for hard tissue repair. It is well know that an appropriate cellular response to bioceramics surfaces is essential for tissue regeneration and integration. As the in vivo implants, the implanted bioceramics are immediately coated with proteins from blood and body fluids, and it is through this coated layer that cells sense and respond to foreign implants. Hence, the adsorption of proteins is critical within the sequence of biological activities. However, the biological mechanisms of the interactions of bioceramics and proteins are still not well understood. In this review, we will recapitulate the recent studies on the bioceramic -protein interactions.

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“…A detailed model describing the likely dependences of the rate constants of protein adsorption and desorption on the fraction of charged lipids in a lipid bilayer are described in Trusova and Gorbenko [133], Rabe et al . [134] and Zhdanov and Kasemo [135].…”

Section: Probing Transmembrane Proteins On Solid Surfacesmentioning

confidence: 99%

Engineering Lipid Bilayer Membranes for Protein Studies

Khan

Dosoky

Williams

2013

IJMS

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Lipid membranes regulate the flow of nutrients and communication signaling between cells and protect the sub-cellular structures. Recent attempts to fabricate artificial systems using nanostructures that mimic the physiological properties of natural lipid bilayer membranes (LBM) fused with transmembrane proteins have helped demonstrate the importance of temperature, pH, ionic strength, adsorption behavior, conformational reorientation and surface density in cellular membranes which all affect the incorporation of proteins on solid surfaces. Much of this work is performed on artificial templates made of polymer sponges or porous materials based on alumina, mica, and porous silicon (PSi) surfaces. For example, porous silicon materials have high biocompatibility, biodegradability, and photoluminescence, which allow them to be used both as a support structure for lipid bilayers or a template to measure the electrochemical functionality of living cells grown over the surface as in vivo. The variety of these media, coupled with the complex physiological conditions present in living systems, warrant a summary and prospectus detailing which artificial systems provide the most promise for different biological conditions. This study summarizes the use of electrochemical impedance spectroscopy (EIS) data on artificial biological membranes that are closely matched with previously published biological systems using both black lipid membrane and patch clamp techniques.

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Electrostatically-controlled protein adsorption onto lipid bilayer: Modeling adsorbate aggregation behavior

Cited by 8 publications

References 57 publications

Argon plasma modified nanocomposite polyurethane scaffolds provide an alternative strategy for cartilage tissue engineering

Argon plasma modified nanocomposite polyurethane scaffolds provide an alternative strategy for cartilage tissue engineering

A review of protein adsorption on bioceramics

Engineering Lipid Bilayer Membranes for Protein Studies

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