Electrostimulation in an autonomous culture lab-on-chip provides neuroprotection of a retinal explant from a retinitis pigmentosa mouse-model

Cabello, Miguel; Mozo, Marta; Cerda, Berta de la; Aracil, Carmen; Díaz-Corrales, Francisco J.; Perdigones, Francisco; Valdés-Sánchez, Lourdes; Relimpio, Isabel; Bhattacharya, Shom Shanker; Quero, J.M.

doi:10.1016/j.snb.2019.02.118

Cited by 12 publications

(9 citation statements)

References 36 publications

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“…Recently, the PCB-based thermocycler for PCR developed in [ 35 ] (2019) required a syringe pump in order to move the DNA sample through a microchannel; the lab-on-PCB reported in [ 36 ] (2019) for organotypic cultures required continuous flow. Therefore, it needed to be connected to a external source (New Era Pump Systems, Inc, Farmingdale, NY, USA) to feed the tissues with culture medium.…”

Section: External Energy Systemsmentioning

confidence: 99%

Lab-on-PCB and Flow Driving: A Critical Review

Perdigones

2021

Micromachines

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Lab-on-PCB devices have been developed for many biomedical and biochemical applications. However, much work has to be done towards commercial applications. Even so, the research on devices of this kind is rapidly increasing. The reason for this lies in the great potential of lab-on-PCB devices to provide marketable devices. This review describes the active flow driving methods for lab-on-PCB devices, while commenting on their main characteristics. Among others, the methods described are the typical external impulsion devices, that is, syringe or peristaltic pumps; pressurized microchambers for precise displacement of liquid samples; electrowetting on dielectrics; and electroosmotic and phase-change-based flow driving, to name a few. In general, there is not a perfect method because all of them have drawbacks. The main problems with regard to marketable devices are the complex fabrication processes, the integration of many materials, the sealing process, and the use of many facilities for the PCB-chips. The larger the numbers of integrated sensors and actuators in the PCB-chip, the more complex the fabrication. In addition, the flow driving-integrated devices increase that difficulty. Moreover, the biological applications are demanding. They require transparency, biocompatibility, and specific ambient conditions. All the problems have to be solved when trying to reach repetitiveness and reliability, for both the fabrication process and the working of the lab-on-PCB, including the flow driving system.

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Section: External Energy Systemsmentioning

confidence: 99%

Lab-on-PCB and Flow Driving: A Critical Review

Perdigones

2021

Micromachines

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“…Local staining with toluidine blue, local application of cholera toxin beta, and transient response to lipopolysaccharide in the retina were all demonstrated. Quero and co-workers created an “autonomous” microfluidic chamber for the culture of mouse retinal explants that incorporated gold microelectrodes, a microheater, and a thermistor (for temperature control), and allowed for optical stimulation and real-time monitoring; the conditions tested had a protective effect on photoreceptor cell death 49 . Such devices could also be applicable to recording and stimulation of other types of electrically excitable tissue, such as muscle.…”

Section: In Vitro Studiesmentioning

confidence: 99%

Microfluidics for interrogating live intact tissues

et al. 2020

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The intricate microarchitecture of tissues – the “tissue microenvironment” – is a strong determinant of tissue function. Microfluidics offers an invaluable tool to precisely stimulate, manipulate, and analyze the tissue microenvironment in live tissues and engineer mass transport around and into small tissue volumes. Such control is critical in clinical studies, especially where tissue samples are scarce, in analytical sensors, where testing smaller amounts of analytes results in faster, more portable sensors, and in biological experiments, where accurate control of the cellular microenvironment is needed. Microfluidics also provides inexpensive multiplexing strategies to address the pressing need to test large quantities of drugs and reagents on a single biopsy specimen, increasing testing accuracy, relevance, and speed while reducing overall diagnostic cost. Here, we review the use of microfluidics to study the physiology and pathophysiology of intact live tissues at sub-millimeter scales. We categorize uses as either in vitro studies – where a piece of an organism must be excised and introduced into the microfluidic device – or in vivo studies – where whole organisms are small enough to be introduced into microchannels or where a microfluidic device is interfaced with a live tissue surface (e.g. the skin or inside an internal organ or tumor) that forms part of an animal larger than the device. These microfluidic systems promise to deliver functional measurements obtained directly on intact tissue – such as the response of tissue to drugs or the analysis of tissue secretions – that cannot be obtained otherwise.

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“…Up to now, lab-on-PCBs include several biomedical applications, for example, for detecting cell viability [ 7 ], molecular diagnosis [ 8 ], organotypic cultures [ 9 ], or electrolytes detection [ 10 ]. Focusing on electrophoresis-based applications, capillary electrophoresis (CE) is the most usual technique in lab on chip [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

2021

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In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA buffer), a microheater for increasing the solubility of the agarose, a negative temperature coefficient (NTC) thermistor for controlling the temperature, a light dependent resistor (LDR) sensor for measuring the transparency of the mixture, and two electrodes for performing the electrophoresis. The agarose preparation functions are governed by a microcontroller. The device requires a PMMA structure to define the wells of the agarose gel, and to release the electrodes from the agarose. The maximum voltage and current that the system requires are 40 V to perform the electrophoresis, and 1 A for activating the microheater. The chosen temperature for mixing is 80 ∘C, with a mixing time of 10 min. In addition, the curing time is about 30 min. This device is intended to be integrated as a part of a larger lab-on-PCB system for DNA amplification and detection. However, it can be used to migrate DNA amplified in conventional thermocyclers. Moreover, the device can be modified for preparing larger agarose gels and performing electrophoresis.

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Electrostimulation in an autonomous culture lab-on-chip provides neuroprotection of a retinal explant from a retinitis pigmentosa mouse-model

Cited by 12 publications

References 36 publications

Lab-on-PCB and Flow Driving: A Critical Review

Lab-on-PCB and Flow Driving: A Critical Review

Microfluidics for interrogating live intact tissues

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

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