Elektronenmikroskopische Lokalisation von Na+ und Cl− in Zellen und Geweben

Komnick, Hans

doi:10.1007/bf01881781

Cited by 236 publications

(47 citation statements)

References 4 publications

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“…We followed a modification of the Komnick andKomnick method (1962,1963) according to Mentre and Escaig (1988). A 2 % pyroantimonate (PA) (RP, KSb(OH)6) (Merck; Darmstadt, FRG) solution was concentrated to 10% by gentle boiling.…”

Section: Methodsmentioning

confidence: 99%

Localization of calcium changes in stimulated rat mast cells.

Horoyan¹,

Solèr²,

Benoliel³

et al. 1992

J Histochem Cytochem.

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We studied intracellular free, bound, and sequestered calcium in rat mast cells after various stimulations. The use of a fluorescent probe combined with digitized imaging on individual living cells demonstrated transient increases of free Ca2+ in the micromolar range. The use of histochemical techniques (K pyroantimonate and anhydrous fmtion), together with X-ray microanalysis, energy electron-loss spectroscopy, and electron spectroscopic imaging, revealed large amounts of stored calcium within the cells (in the millimolar range). Chelation experiments and stimulations enabled us to identify at least two pools of bound calcium which exhibited different dynamic behaviors. Stimulation in the pres-

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Section: Methodsmentioning

confidence: 99%

Localization of calcium changes in stimulated rat mast cells.

Horoyan¹,

Solèr²,

Benoliel³

et al. 1992

J Histochem Cytochem.

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“…For the cytochemical demonstration of chloride ions, tissues were treated essentially according to the method of KoMNICK (1962). They were fixed in chilled 1% 0504-silver lactate mixture containing 0.25 M sucrose, dehydrated rapidly through a series of acetone and embedded in epoxy resin.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructure and chloride cytochemistry of the hindgut epithelium in the larvae of the seawater mosquito, Aedes togoi Theobald.

Asakura

1982

Arch. Histol. Jap., Arch. Histol. Jpn

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“…The pyroantimonate technique was first used to demonstrate the presence of sodium ions (Komnick, 1962) but it has since been shown that pyroantimonate forms complexes with other cations. In their review of the technique, Wick & Hepler (1982) noted that when X-ray microanalysis was also used after pyroantimonate, calcium was either the only cellular cation associated with precipitates (13 references cited) or the major cationic component (6 references cited).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural localization of calcium ions in ram spermatozoa before and after cold shock as demonstrated by a pyroantimonate technique

Plummer¹,

Watson²

1985

Reproduction

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Summary. Ram spermatozoa were subjected to cold shock before fixation in pyroantimonate\p=n-\osmium. Ultrathin sections revealed an electron-dense particulate precipitate in association with the cells. The precipitate was shown to be related to the presence of calcium by exposure of the material to EGTA which reduced or completely eliminated the deposits.In the acrosome region, very little precipitate was evident when the plasma membrane was intact. Cold shock resulted in the disruption of the plasma membrane. When the acrosome remained intact, precipitate was concentrated just anterior to the equatorial segment, but many cells also had acrosomal disruption and then a more even distribution of precipitate was seen on the outer acrosomal membrane. Precipitate was rarely visible within or beneath the acrosome. Post-acrosomally, calcium pyroantimonate deposits were frequently present in the dense lamina beneath the plasma membrane and these became more intense after cold shock.Midpiece sections revealed a few large granules beneath the plasma membrane and a fine particulate precipitate within mitochondria. Similarly, the fine precipitate was also associated with the outer dense fibres in midpieces and tails. Cold shock did not apparently increase the extent or intensity of precipitates in these sites.

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Elektronenmikroskopische Lokalisation von Na+ und Cl− in Zellen und Geweben

Cited by 236 publications

References 4 publications

Localization of calcium changes in stimulated rat mast cells.

Localization of calcium changes in stimulated rat mast cells.

Ultrastructure and chloride cytochemistry of the hindgut epithelium in the larvae of the seawater mosquito, Aedes togoi Theobald.

Ultrastructural localization of calcium ions in ram spermatozoa before and after cold shock as demonstrated by a pyroantimonate technique

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