Elektronenmikroskopische Untersuchungen �ber das Verhalten eines vom Kalb isolierten Parainfluenza-3-Virus in K�lbernierenzellkulturen

Reczko, E; Boegel, K

doi:10.1007/bf01241474

Cited by 22 publications

(4 citation statements)

References 10 publications

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“…These nuclear filaments are comparable to those seen in swine poxvirus (Reczko, 1962;Conroy & Meyer, 1971 ;Kim et al I977) and Junco poxvirus (Beaver & Cheatham, 1963) infections and could resemble part of the nuclear protein matrix (Wunderlich, 1978 ).…”

Section: Discussionsupporting

confidence: 61%

Nuclear Changes in Cells Infected with Parapoxviruses Stomatitis Papulosa and Orf: an in vivo and in vitro Ultrastructural Study

Pospischil

Bachmann

1980

Journal of General Virology

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SUMMARYDuring ultrahistological investigations of naturally occurring cases of stomatitis papulosa in cattle and Orf in sheep, nuclear changes consisting of aggregations of double membrane-containing tubular structures (outer diam. IOO to I3O nm, inner diam. 50 to 65 nm) and filamentous material were observed. These changes could be reproduced in vitro after infection of bovine (BEL) and ovine (OEL) embryonic lung cell cultures with stomatitis papulosa virus and Orf virus isolates. Nuclear tubules were mostly associated with stomatitis papulosa, whereas filaments were regularly detected in Orfvirus infections in vivo. Stomatitis papulosa virus also induced nuclear tubules in vitro in the two cell culture types employed, whereas tubular structures after Off virus infection only developed in ovine embryonic lung cell cultures in addition to filamentous structures. Off virus infection of BEL cell cultures induced the formation of filaments. Fluorescent antibody staining revealed parapoxvirus-specific antigens only in the cytoplasm of infected cells.

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Section: Discussionsupporting

confidence: 61%

Nuclear Changes in Cells Infected with Parapoxviruses Stomatitis Papulosa and Orf: an in vivo and in vitro Ultrastructural Study

Pospischil

Bachmann

1980

Journal of General Virology

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“…Both intracytoplasmic and intranuclear inclusion bodies consisting of NCs are often found in members of the genus Morbillivirus (MURPHY et al, 1995). In addition, intranuclear inclusions of NCs have previously been assumed to occur in bovine parainfluenza virus type 3 (BPIV-3), a member of the genus Paramyxovirus (REISINGER et al, 1959;BÖ GEL and KLINGER, 1961;RECZKO and BÖ GEL, 1962;MCLEAN and DOANE, 1971;. In rubulaviruses, including NDV, intranuclear inclusions have not yet been found.…”

Section: Discussionmentioning

confidence: 99%

Intranuclear Inclusions in Cells Infected with Newcastle Disease Virus

Granzow¹,

Weiland

Mundt

et al. 1999

Journal of Veterinary Medicine, Series B

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Cells infected by Newcastle Disease Virus were observed to contain both intracytoplasmic and intranuclear inclusion bodies. Ultrastructurally, they consisted of twisted strands of about 18-20 nm diameter resembling nucleocapsids. The presence of these inclusions was detected irrespective of host cell or pathogenicity of the virus. In immunofluorescence and immunogold labelling experiments, these structures were tagged by an anti-P protein monoclonal antibody. In summary, we show that intracytoplasmic and intranuclear inclusion bodies, hitherto used as a taxonomic characteristic for the genus Morbillivirus of the Paramyxoviridae, also occur in a member of the genus Rubulavirus.

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“…ruses, notably parainfluenza 1 (Sendai), have shown cytopathology essentially similar to that of parainfluenza 2 virus (8,27). The bovine strain of parainfluenza 3 (SF-4), however, produces intranuclear eosinophilic inclusions (7,20,25,29), a feature not generally characteristic of human parainfluenza viruses.…”

Section: Afmentioning

confidence: 99%

Morphogenesis of Type 2 Parainfluenza Virus Examined by Light and Electron Microscopy

Howe

Morgan

Cyr

et al. 1967

J Virol

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The development of type 2 parainfluenza virus in HeLa and stable human amnion cells was examined by use of antisera labeled with fluorescein and ferritin. Serum containing antibody predominantly to soluble viral antigen gave specific fluorescence which was first detectable in small cytoplasmic foci 8 to 10 hr after initiation of infection. By 20 to 24 hr, when the production of infective virus and hemagglutinin was maximal, large perinuclear aggregates of fluorescence were observed which corresponded in distribution and time of appearance to the eosinophilic inclusions seen in similar preparations stained with azure eosin. The inclusions, examined by electron microscopy, were composed of fibrils, presumably viral ribonucleoprotein, which specifically bound the antibody labeled with ferritin. With antiserum to concentrated virus, on the other hand, specific fluorescence was most marked at the surface of infected cells. Foci of fluorescence at the surface represented segments of membrane which had become differentiated morphologically and antigenically to resemble the viral envelope. These were the sites where mature virions appeared. The latter exhibited marked pleomorphism; in some instances, particles were formed which lacked recognizable internal fibrils but which possessed an enclosing membrane bearing viral antigen. Filamentous forms showing an organized internal structure were also observed at the cell surface, but were never encountered in negatively stained preparations. No clear relationship between these filaments and the spherical or oval forms could be established. In negatively stained preparations, nucleocapsid released by rupture of viral particles was similar in appearance to that reported for other paramyxoviruses. It seems probable that this component has a helical configuration.

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Elektronenmikroskopische Untersuchungen �ber das Verhalten eines vom Kalb isolierten Parainfluenza-3-Virus in K�lbernierenzellkulturen

Cited by 22 publications

References 10 publications

Nuclear Changes in Cells Infected with Parapoxviruses Stomatitis Papulosa and Orf: an in vivo and in vitro Ultrastructural Study

Nuclear Changes in Cells Infected with Parapoxviruses Stomatitis Papulosa and Orf: an in vivo and in vitro Ultrastructural Study

Intranuclear Inclusions in Cells Infected with Newcastle Disease Virus

Morphogenesis of Type 2 Parainfluenza Virus Examined by Light and Electron Microscopy

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