Elektronenmikroskopische Untersuchungen �ber die Initialstadien der Vaccine-Virusinfektion von HeLa-Zellen

Nielsen, G.; Peters, D.

doi:10.1007/bf01242157

Cited by 14 publications

(1 citation statement)

References 19 publications

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“…Electron microscopic examination of reovirus (9), vaccinia virus (5,1,7,10,25), adenovirus (5), Newcastle disease virus (24,31), parainfluenza virus SV5 (4), and influenza virus (8) suggests that viral particles gain entry into the host cell by a process of phagocytosis or "viropexis," as originally suggested by Fazekas de St. Groth (12). Studies of herpes simplex virus also have yielded results that have been interpreted as being con-sistent with this concept (11,13,30).…”

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confidence: 84%

Electron Microscopy of Herpes Simplex Virus

Morgan

Rose

Mednis

1968

J Virol

187

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Although capsids of herpes simplex virus were encountered within phagocytic vesicles, they were more commonly observed free within the cytoplasm. Stages in the release of virus from vesicles were not seen. There appeared to be five distinct steps in the process whereby the virus initiates infection: attachment, digestion of the viral envelope, digestion of the cell wall, passage of the capsid directly into the cytoplasm, and digestion of the capsid with release of the core. Antibody probably interferes with the first two stages. This paper describes and illustrates the probable manner in which herpes simplex virus (Herpesvirus hominis) initiates cellular infection. The three ensuing papers are concerned with the sequential stages of viral development, the effects of blocking DNA synthesis, and the antigenic alteration of host cell components. Since the initial examination of herpes simplex virus in thin sections (21, 22) a confusing nomenclature has arisen, including such descriptive terms as internal body, nucleoid, central body, internal membrane, peripheral coat, second membrane, single membrane form, naked particle, double membrane form, and complete virus. ["Nucleoid" was originally coined to describe the dense, eccentrically placed structure in immature forms of vaccinia and fowl pox viruses (23). It has become an unfortunate misnomer when applied to the dense cores characterictic of the majority of viruses.l Application of the negative staining technique, however, has now provided more precise information about the structure of the virus (33), which indicates that it consists of a core surrounded by a capsid. The capsid is icosahedral in shape and is composed of 162 capsomeres arranged in 5:3:2 axial symmetry. Capsids may or may not be enclosed within an envelope that is devoid of clearly defined subunits. Applying this nomenclature (19) to the appearance of the virus in thin sections, one can recognize a clearly defined core, a capsid (the first or inner membrane), and an envelope (the second or peripheral membrane). These terms will be used henceforth.

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