A biomonitoring study of air pollution was developed in an urban-industrial area (Seixal, Portugal) using leaves of strawberry plants (Fragaria × ananassa Duchesne ex Rozier) as biomonitors to identify the main sources and hotspots of air pollution in the study area. The distribution of exposed strawberry plants in the area was based on a citizen science approach, where residents were invited to have the plants exposed outside their homes. Samples were collected from a total of 49 different locations, and their chemical composition was analyzed for 22 chemical elements using X-ray Fluorescence spectrometry. Source apportionment tools, such as enrichment factors and principal component analysis (PCA), were used to identify three different sources, one geogenic and two anthropogenic (steel industry and traffic), besides plant major nutrients. The spatial distribution of elemental concentrations allowed the identification of the main pollution hotspots in the study area. The reliability of using strawberry leaves as biomonitors of air pollution was evaluated by comparing them with the performance of transplanted lichens by regression analysis, and a significant relation was found for Fe, Pb, Ti, and Zn, although with a different accumulation degree for the two biomonitors. Furthermore, by applying PCA to the lichen results, the same pollution sources were identified.