Elevated ATP, cytokines and potential microglial inflammation distinguish exfoliation glaucoma from exfoliation syndrome

Pulukool, Sujith Kumar; Bhagavatham, Sai Krishna Srimadh; Kannan, Vishnu; Parim, Brahmanaidu; Challa, Sivateja; Karnatam, Vidya; M., Datta Darshan V.; Mir, Irfan Ahmad; Sukumar, Piruthivi; Vijayalakshmi, V.; Sharma, Abha; Sivaramakrishnan, Venketesh

doi:10.1016/j.cyto.2022.155807

Cited by 9 publications

(2 citation statements)

References 84 publications

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“…TNF-α is a cytokine that initiates immune-mediated inflammation response . It is an important biomarker in diseases like congenital heart disease, dengue, rheumatoid arthritis, glaucoma, − to name a few. To study its regulation, enzyme linked immuno-sorbent assay (ELISA) is utilized as a well-known, reliable, and gold-standard plate-based technique.…”

Section: Resultsmentioning

confidence: 99%

Tecoma stans Floral Extract-Based Biosynthesis for Enhanced Surface Plasmon-Coupled Emission and a Preliminary Study on Fluoroimmunoassay

Rathnakumar,

Bhaskar,

Sivaramakrishnan

et al. 2024

Anal. Chem.

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We demonstrate the synthesis of biogenic supported silver spiked star architectures and their application to increase the electromagnetic field intensity at its tips that enhance plasmoncoupled emission. Tecoma stans floral extract has been used to synthesize silver nanocubes and spiked stars. We observe ∼445-fold and ∼680-fold enhancements in spacer and cavity configurations, respectively, in the SPCE platform. The hotspot intensity and Purcell factor are evaluated by carrying out finite-difference time-domain (FDTD) simulations. Time-based studies are presented to modulate the sharpness of the edges wherein an increase in the tip sharpness with the increase in reaction time up to 5 h is observed. The unique morphology of the silver architectures allowed us to utilize them in biosensing application. A SPCE-based fluoroimmunoassay was performed, achieving a 1.9 pg/mL limit of detection of TNF-α cytokine. This combination of anisotropic architectures, SPCE and immunoassay prove to be a powerful platform for the ultrasensitive detection of biomarkers in surface-bound assays.

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Section: Resultsmentioning

confidence: 99%

Tecoma stans Floral Extract-Based Biosynthesis for Enhanced Surface Plasmon-Coupled Emission and a Preliminary Study on Fluoroimmunoassay

Rathnakumar,

Bhaskar,

Sivaramakrishnan

et al. 2024

Anal. Chem.

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“…Metabolomic analysis of different kinds of samples of AD patients also indicates deregulation of the Krebs cycle, mitochondrial function, neurotransmitter and amino acid metabolism, and lipid biosynthesis pathways 46 – 48 . Multi-omic analysis and validation has helped to understand mechanisms, biomarkers and potential therapeutic targets in osteonecrosis, rheumatoid arthritis, glaucoma 49 – 51 .…”

Section: Introductionmentioning

confidence: 99%

Integrated multi-omics analysis of Alzheimer’s disease shows molecular signatures associated with disease progression and potential therapeutic targets

Kodam

Swaroop

Pradhan

et al. 2023

Sci Rep

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Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by the formation of amyloid plaques implicated in neuronal death. Genetics, age, and sex are the risk factors attributed to AD. Though omics studies have helped to identify pathways associated with AD, an integrated systems analysis with the available data could help to understand mechanisms, potential biomarkers, and therapeutic targets. Analysis of transcriptomic data sets from the GEO database, and proteomic and metabolomic data sets from literature was performed to identify deregulated pathways and commonality analysis identified overlapping pathways among the data sets. The deregulated pathways included those of neurotransmitter synapses, oxidative stress, inflammation, vitamins, complement, and coagulation pathways. Cell type analysis of GEO data sets showed microglia, endothelial, myeloid, and lymphoid cells are affected. Microglia are associated with inflammation and pruning of synapses with implications for memory and cognition. Analysis of the protein-cofactor network of B2, B6, and pantothenate shows metabolic pathways modulated by these vitamins which overlap with the deregulated pathways from the multi-omics analysis. Overall, the integrated analysis identified the molecular signature associated with AD. Treatment with anti-oxidants, B2, B6, and pantothenate in genetically susceptible individuals in the pre-symptomatic stage might help in better management of the disease.

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Integrated clinical and metabolomic analysis of dengue infection shows molecular signatures associated with host-pathogen interaction in different phases of the disease

et al. 2023

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Elevated ATP, cytokines and potential microglial inflammation distinguish exfoliation glaucoma from exfoliation syndrome

Cited by 9 publications

References 84 publications

Tecoma stans Floral Extract-Based Biosynthesis for Enhanced Surface Plasmon-Coupled Emission and a Preliminary Study on Fluoroimmunoassay

Tecoma stans Floral Extract-Based Biosynthesis for Enhanced Surface Plasmon-Coupled Emission and a Preliminary Study on Fluoroimmunoassay

Integrated multi-omics analysis of Alzheimer’s disease shows molecular signatures associated with disease progression and potential therapeutic targets

Integrated clinical and metabolomic analysis of dengue infection shows molecular signatures associated with host-pathogen interaction in different phases of the disease

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