Elevated expression of N-acetylglucosaminyltransferase V in first trimester human placenta

Tomiie, Mari; Isaka, Shigeyuki; Miyoshi, Eiji; Taniguchi, Naoyuki; Kimura, Tadashi; Ogita, Kazuhide; Tsutsui, Tateki; Shimoya, Koichiro; Nakagawa, Takatoshi; Kondo, Akihiro; Koyama, Masayasu; Murata, Yuji

doi:10.1016/j.bbrc.2005.02.186

Cited by 15 publications

(11 citation statements)

References 18 publications

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“…As a negative control, the primary antibody was replaced with normal mouse IgG at an appropriate dilution. As a positive control, tissue sections of normal placenta were used as reported previously (Tomiie et al, 2005). N-acetylglucosaminyltransferase V expression levels were classified semiquantitatively based on the total scores of the per cent positivity of stained tumour cells and the staining intensity.…”

Section: Skntdffigkptilreltsmentioning

confidence: 99%

Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis

et al. 2007

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N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyses b1 -6 branching of N-acetylglucosamine on asparagine-linked oligosaccharides of cell proteins. The present study aimed to investigate GnT-V expression and its prognostic significance in endometrial cancer. N-acetylglucosaminyltransferase V expression was studied by immunohistochemistry in 74 surgically resected endometrial cancers, and the staining intensity was evaluated. High GnT-V expression in tumour cells was found in 43 (58.1%) of the 74 cases, and was positively correlated with advanced patient age, histological grade, and lymph vascular space involvement. Patients with high GnT-V expression had significantly impaired overall survival and progression-free survival (PFS) (P ¼ 0.0041 and P ¼ 0.0023, respectively) compared to patients with low expression of GnT-V. On multivariate analysis, GnT-V expression was an independent prognostic factor for PFS (P ¼ 0.0364). b1 -6 branching of asparagine-linked oligosaccharides was also detected in GnT-V-positive endometrial cancer cells by leukoagglutinating phytohaemagglutinin (L 4 -PHA) staining, and the molecular size of the major glycoproteins recognised by L 4 -PHA was approximately 60 -200 kDa by lectin blot analysis. These results suggested that high GnT-V expression was correlated with an unfavourable clinical outcome, and that GnT-V is involved in the malignant potential of endometrial cancer by increasing the synthesis of b1 -6 branching of asparagine-linked oligosaccharides.

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Section: Skntdffigkptilreltsmentioning

confidence: 99%

Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis

et al. 2007

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“…Induction or inhibition of these oligosaccharides in such cell lines, using chemical or genetic methods has been shown to result in a corresponding acquisition or inhibition of the metastatic phenotype, respectively [12][13][14][15]. Their expression on highly invasive cancers [16,17] and on normal cells involved in invasive functions [18] suggests their involvement in the process of invasion; however, the precise mechanism behind this remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Poly N-acetyllactosamine substitutions on N- and not O-oligosaccharides or Thomsen–Friedenreich antigen facilitate lung specific metastasis of melanoma cells via galectin-3

et al. 2008

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Galectin-3 on vascular endothelium has been shown to facilitate lung specific metastasis. Metastatic variants of B16 melanoma were chosen to identify specific ligands that mediate lung colonization via galectin-3. Flow cytometry showed that, galectin-3 binding to cells correlates with surface expression of poly N-acetyllactosamine (polylacNAc) but not with other reported ligands, e.g. Thomsen-Friedenreich (T/Tn) antigen. Immobilized galectin-3 promoted adhesion of melanoma cells in a metastasis dependent manner. Moreover, adhesion and galectin-3 binding to cells were specifically inhibited with lactose. These properties together with lung metastasis were inhibited with N-glycosylation inhibitor Swainsonine (SW), whereas, O-glycosylation inhibitor Benzyl-alpha-N-acetylgalactosamine (BG) had no effect. BG treatment significantly increased expression of T/Tn antigen on low metastatic cells; however, had no effect on their metastatic potential. The studies very comprehensively demonstrate the importance of polylacNAc substitutions on N-oligosaccharides in galectin-3 mediated lung metastasis.

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“…Furthermore, a higher expression of MGAT5 in first trimester placentas than those at term was determined [24].…”

Section: Introductionmentioning

confidence: 99%

N-acetylglucosaminyltransferase V inhibits the invasion of trophoblast cells by attenuating MMP2/9 activity in early human pregnancy

et al. 2015

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Elevated expression of N-acetylglucosaminyltransferase V in first trimester human placenta

Cited by 15 publications

References 18 publications

Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis

Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis

Poly N-acetyllactosamine substitutions on N- and not O-oligosaccharides or Thomsen–Friedenreich antigen facilitate lung specific metastasis of melanoma cells via galectin-3

N-acetylglucosaminyltransferase V inhibits the invasion of trophoblast cells by attenuating MMP2/9 activity in early human pregnancy

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