Elevated miR-143 and miR-34a gene expression in human visceral adipose tissue are associated with insulin resistance in non-diabetic adults: a cross-sectional study

Yuzbashian, Emad; Zani, Stepheny C. de Campos; Zarkash, Maryam; Asghari, Golaleh; Hedayati, Mehdi; Khalaj, Alireza; Chan, Catherine B.

doi:10.1007/s40519-022-01476-6

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…Contradictory data also exist regarding mir-143 and miR-223 from circulation. Peripheral blood levels of miR-143 have been found down-regulated in the of patients with gestational diabetes [ 78 ], whereas elevated levels of miR-143 gene expression were correlated with the degree of obesity and proposed as predictor of insulin resistance in people without diabetes [ 107 ]. In line with previous studies, we also described a slightly increased level of miR-143 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Microvesicle-associated and circulating microRNAs in diabetic dyslipidemia: miR-218, miR-132, miR-143, and miR-21, miR-122, miR-155 have biomarker potential

Nemecz,

Stefan,

Comarița

et al. 2023

Cardiovasc Diabetol

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Background Circulating MicroRNAs (miRNAs) carried by microvesicles (MVs) have various physiological and pathological functions by post-transcriptional regulation of gene expression being considered markers for many diseases including diabetes and dyslipidemia. We aimed to identify new common miRNAs both in MVs and plasma that could be predictive biomarkers for diabetic dyslipidemia evolution. Methods For this purpose, plasma from 63 participants in the study (17 type 2 diabetic patients, 17 patients with type 2 diabetes and dyslipidemia, 14 patients with dyslipidemia alone and 15 clinically healthy persons without diabetes or dyslipidemia) was used for the analysis of circulating cytokines, MVs, miRNAs and MV-associated miRNAs. Results The results uncovered three miRNAs, miR-218, miR-132 and miR-143, whose expression was found to be significantly up-regulated in both circulating MVs and plasma from diabetic patients with dyslipidemia. These miRNAs showed significant correlations with important plasma markers, representative of this pathology. Thus, MV/plasma miR-218 was negatively correlated with the levels of erythrocyte MVs, plasma miR-132 was positively connected with MV miR-132 and negatively with uric acid and erythrocyte plasma levels, and plasma miR-143 was negatively related with creatinine levels and diastolic blood pressure. Also, three miRNAs common to MV and plasma, namely miR-21, miR-122, and miR-155, were identified to be down-regulated and up-regulated, respectively, in diabetic dyslipidemia. In addition, MV miR-21 was positively linked with cholesterol plasma levels and plasma miR-21 with TNFα plasma levels, MV miR-122 was negatively correlated with LDL-c levels and plasma miR-122 with creatinine and diastolic blood pressure and positively with MV miR-126 levels, MV miR-155 was positively associated with cholesterol and total MV levels and negatively with HDL-c levels, whereas plasma miR-155 was positively correlated with Il-1β plasma levels and total MV levels and negatively with MV miR-223 levels. Conclusions In conclusion, miR-218, miR-132, miR-143, and miR-21, miR-122, miR-155 show potential as biomarkers for diabetic dyslipidemia, but there is a need for more in-depth studies. These findings bring new information regarding the molecular biomarkers specific to diabetic dyslipidemia and could have important implications for the treatment of patients affected by this pathology.

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Section: Resultsmentioning

confidence: 99%

Microvesicle-associated and circulating microRNAs in diabetic dyslipidemia: miR-218, miR-132, miR-143, and miR-21, miR-122, miR-155 have biomarker potential

Nemecz,

Stefan,

Comarița

et al. 2023

Cardiovasc Diabetol

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Decoding visceral adipose tissue molecular signatures in obesity and insulin resistance: a multi‐omics approach

Roy,

Ghosh,

Ghosh

et al. 2024

Obesity

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ObjectiveObesity‐associated insulin resistance (IR) is responsible for considerable morbidity and mortality globally. Despite vast genomic data, many areas, from pathogenesis to management, still have significant knowledge gaps. We aimed to characterize visceral adipose tissue (VAT) in obesity and IR through a multi‐omics approach.MethodsWe procured data on VAT samples from the Gene Expression Omnibus (GEO) for the following two groups: 1) populations with obesity (n = 34) versus those without (n = 26); and 2) populations with obesity and IR (n = 15) versus those with obesity but without IR (n = 15). Gene set enrichment, protein‐protein interaction network construction, hub gene identification, and drug‐gene interactions were performed, followed by regulatory network prediction involving transcription factors (TFs) and microRNAs (miRNAs).ResultsInterleukin signaling pathways, cellular differentiation, and regulation of immune response revealed a significant cross talk between VAT and the immune system. Other findings include cancer pathways, neurotrophin signaling, and aging. A total of 10 hub genes, i.e., STAT1, KLF4, DUSP1, EGR1, FOS, JUN, IL2, IL6, MMP9, and FGF9, 24 TFs, and approved hub gene‐targeting drugs were obtained. A total of 10 targeting miRNAs (e.g., hsa‐miR‐155‐5p, hsa‐miR‐34a‐5p) were associated with obesity and IR‐related pathways.ConclusionsOur multi‐omics integration method revealed hub genes, TFs, and miRNAs that can be potential targets for investigation in VAT‐related inflammatory processes and IR, therapeutic management, and risk stratifications.image

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Obesity and noncoding RNAs in epigenetic inheritance of metabolic disease

Zhang,

Ren,

et al. 2025

Nutrition in the Control of Inflammation

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Elevated miR-143 and miR-34a gene expression in human visceral adipose tissue are associated with insulin resistance in non-diabetic adults: a cross-sectional study

Cited by 3 publications

References 30 publications

Microvesicle-associated and circulating microRNAs in diabetic dyslipidemia: miR-218, miR-132, miR-143, and miR-21, miR-122, miR-155 have biomarker potential

Microvesicle-associated and circulating microRNAs in diabetic dyslipidemia: miR-218, miR-132, miR-143, and miR-21, miR-122, miR-155 have biomarker potential

Decoding visceral adipose tissue molecular signatures in obesity and insulin resistance: a multi‐omics approach

Obesity and noncoding RNAs in epigenetic inheritance of metabolic disease

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