Elevated neutrophil respiratory burst activity in essential hypertensive patients

Abstract

Neutrophils play a significant role in maintaining the integrity of innate immunity via their potent respiratory burst activity. However, the uncontrolled activation of respiratory burst in neutrophils also attributes to chronic diseases such as primary hypertension and atherosclerosis. In our study, we have investigated the activation of respiratory burst function of neutrophils harvested from essential hypertensive patients. In the presence of stimuli PMA and opsonized zymosan (OZ), hypertensive patients' neutrophils secrete significantly higher amount of superoxide anions compared to normotensive control. Although the magnitude of activation varies between both groups, yet the kinetics of activation is similar. When normotensive control's neutrophils were pre-treated with hypertensive serum, the cells failed to migrate toward fMLP which indicates the impairment of the migration property. In conclusion, the respiratory burst activity of neutrophils is affected by hypertension and their elevated superoxide anions production could be an aggravating factor in hypertension-related complication.

Genetic variations of HSPA1A, the heat shock protein levels, and risk of atherosclerosis

“…A close relationship has been demonstrated between a high PMN count and increased cardiovascular risk (Horne et al 2005), and patients with unstable angina or acute myocardial infarction moreover present activated PMNs that release free oxygen radicals and inflammatory mediators which in turn interact with the endothelial cells-triggering the development of atherosclerosis (Baetta and Corsini 2010). Furthermore, circulating PMNs have been shown to be activated in a number of conditions associated with a high risk of developing atherosclerosis, such as hypertension (Ramasamy et al 2010), type 2 diabetes (Hand et al 2007), or hyperlipidemia (Mazor et al 2008). The causal role of PMNs in atherogenesis and their implication in plaque vulnerability ) are presently widely accepted.…”

Association between circulating specific leukocyte types and blood pressure: the Atherosclerosis Risk in Communities (ARIC) study

“…Oxidative stress in hypertensive patients' neutrophils is evidenced by an increased NADPH oxidase production and lipid peroxidation and decreased cytosolic and mitochondrial superoxide dismutase concentration 42,43,44,45. Recently, Ramasamy et al46 evaluated the effect of neutrophil oxidative burst activity in essential hypertensive patients. They found that freshly isolated neutrophils from uncontrolled human subjects diagnosed with essential hypertension acquire the ability of producing a higher amount of ROS in response to stimuli indicating that oxidative bust activity is impaired.…”

Human mesenchymal stem cells protect neutrophils from serum-deprived cell death

“…Supernatant from dextran sedimentation was collected and centrifuged at 1800 rev./min for 10 min at room temperature. The remnant RBC was lysed using hypotonic lysing procedure to obtain the pure PMN population (Ramasamy et al, 2010). The morphological examination and cell count were performed to determine the cell count and purity of the PMN.…”