Elevated Secretory IgA Antibodies to Epstein-Barr Virus (EBV) and Presence of EBV DNA and EBV Receptors in Patients with Cervical Carcinoma

Thoe, Su Yun Se; Wong, Kum-Thong; Pathmanathan, R; Sam, Choon‐Kook; Cheng, Hwee Ming; Prasad, U.

doi:10.1006/gyno.1993.1187

Cited by 23 publications

(16 citation statements)

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“…Our findings are in agreement with other authors such as Se Thoe et al (15), Landers et al (14), and more recently, Sasagawa et al (16), who showed similar results.…”

Section: Discussionsupporting

confidence: 94%

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Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

Santos

Villanova²,

Andrade³

et al. 1994

Oncol Rep

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Abstract. In the present study, our aim was to investigate whether EBV DNA could be found in association with invasive and pre-invasive cervical cancer lesions. We hypothesize that EBV is not merely a commensal agent when present in malignant cervical lesions. DNA was extracted from cervical scrapings followed by nested PCR-based amplification. The patients were 66 women with high grade cervical intraepithelial neoplasia and 14 women with invasive cervical cancer. The control group consisted of 89 women with a normal Pap smear and colposcopy as well as a negative HPV DNA test. Analysis of our results, in conjunction with the work of other authors, leads us to propose that EBV is not merely a commensal agent when present in malignant cervical lesions. The presence of DNA from EBV is significantly associated with cervical cancer.

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“…Our findings are in agreement with other authors such as Se Thoe et al (15), Landers et al (14), and more recently, Sasagawa et al (16), who showed similar results.…”

Section: Discussionsupporting

confidence: 94%

“…Se Thoe et al (15) were able to identify EBV in 63% of malignant cervical tissues, and more recently, Sasagawa et al (16) showed EBV in 55% of cervical cancers and in 26% of normal cervix tissues.…”

Section: Introductionmentioning

confidence: 98%

Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

Santos

Villanova²,

Andrade³

et al. 1994

Oncol Rep

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“…These results concur with those found in a recent (18). A similar increase in prevalence of cervical EBV DNA shedding has been reported in other studies as well (11,12,26). Sasagawa et al evaluated EBV mRNA expression at the cervix and found that the EBV genes EBER-1, LMP-1, and EBNA-2 were expressed significantly more frequently in tissue samples from CIN and cancer patients than women with normal cervices (19).…”

Section: Discussionsupporting

confidence: 68%

Shedding of Epstein-Barr Virus and Cytomegalovirus from the Genital Tract of Women in a Periurban Community in Andhra Pradesh, India

et al. 2011

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We found a large number of false-positive readings by visual inspection with acetic acid (VIA) in a study of cervical cancer screening strategies (VIA, human papillomavirus HPV DNA testing, and Pap cytology) in a periurban community in Andhra Pradesh, India. We evaluated whether these false-positive readings might be occurring as a result of infections with Epstein-Barr virus (EBV) or cytomegalovirus (CMV), prevalent latent herpesviruses known to be shed from the female genital tract. While we found that there was no association between VIA results and the presence of EBV or CMV in the cervix, we did find a high prevalence of both viruses: 20% for EBV and 26% for CMV. In multivariate analyses, CMV prevalence was associated with younger age, lack of running water in the home, and visually apparent cervical inflammation. EBV prevalence was associated with older age and a diagnosis of cervical intraepithelial neoplasia grade 1 or greater. The biological and clinical implications of these viruses at the cervix remain to be determined. The strong association between the presence of EBV and cervical disease warrants future exploration to determine whether EBV plays a causal role in disease development or if it is merely a bystander in the process.We conducted a study of cervical cancer screening strategies in a periurban community near Hyderabad in the state of Andhra Pradesh in southern India. Cervical cells were collected from women for cytological and virological investigation, and the cervix was examined with naked eye visual inspection after application of acetic acid (VIA). We found that a positive VIA reading (presence of an acetowhite lesion in the cervical transformation zone) had a surprisingly low sensitivity (26.3%) and moderate specificity (76.4%) for the detection of cervical neoplasia (9,28).Given the large number of false-positive VIA readings, we inquired if these lesions might be occurring as a result of infections with Epstein-Barr virus (EBV) or cytomegalovirus (CMV), prevalent latent herpesviruses which are known to be shed from the female genital tract (2, 6, 14, 21). We also examined whether cervical EBV and CMV detection correlated with either cervical HPV detection or other markers of cervical pathology, since these viruses have been postulated to have a possible cocarcinogenic role with human papillomavirus (HPV) for cervical neoplasia (19, 23). MATERIALS AND METHODSStudy participants. The study subjects were a subpopulation of the CATCH (Community Access to Cervical Health) project, a population-based screening study in a periurban community near Hyderabad, in the state of Andhra Pradesh, India. The CATCH project aimed to enroll all eligible women 25 years of age and older who were not pregnant and who had not had a hysterectomy. A total of 2,331 women, or approximately 40% of those eligible, were enrolled and consented to an interviewer-administered questionnaire to collect data on demographics, reproductive health, cervical cancer screening history, and smoking status. Participants also pr...

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“…In one study, the EBV Bam HI “W” fragment was detected using polymerase chain reaction (PCR) in 43% of cervical carcinoma, 8% of cervical intraepithelial neoplasia (CIN), and 0% of normal cervices (1) . In another study, 63% of cervical cancer specimens were positive for the EBV genome using DNA in situ hybridization (14) . Tseng et al detected EBV late membrane protein DNA sequences in 26.7% of cases of squamous cell carcinoma (SCC) of the cervix using PCR, and Sasagawa et al recently confirmed the EBV gene expression in CIN and cervical cancer by reverse–transcription polymerase chain reaction and RNA in situ hybridization (2,15) .…”

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confidence: 99%

Epstein-Barr virus plays little role in cervical carcinogenesis in Korean women

Seo¹,

Kim²,

Song³

et al. 2005

Int J Gynecol Cancer

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We examined whether Epstein-Barr virus (EBV) infection plays a role in cervical carcinogenesis in Korean women. EBV infection was examined using polymerase chain reaction (PCR) with two different primer pairs flanking the BamHI "W" fragment of EBV and by EBV-encoded small RNAs (EBER) in situ hybridization in various histologic types of cervical cancer, including 17 cases of squamous cell carcinoma, 36 cases of adenocarcinoma, and 3 cases of small-cell carcinoma. We also evaluated 20 cases of cervical intraepithelial neoplasia and 20 cases of normal uterine cervix. One case of squamous cell carcinoma and three cases of cervical intraepithelial neoplasia were positive for EBV DNA using PCR, but EBER in situ hybridization analysis showed that none of the PCR-positive cases expressed EBER. EBV DNA was not found using PCR in any of the 20 normal uterine cervices. From our results, EBV infection does not seem to play a role in cervical carcinogenesis in Korean women.

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Elevated Secretory IgA Antibodies to Epstein-Barr Virus (EBV) and Presence of EBV DNA and EBV Receptors in Patients with Cervical Carcinoma

Cited by 23 publications

References 0 publications

Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

Shedding of Epstein-Barr Virus and Cytomegalovirus from the Genital Tract of Women in a Periurban Community in Andhra Pradesh, India

Epstein-Barr virus plays little role in cervical carcinogenesis in Korean women

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