Elevated serum levels of pantropic p53 proteins in chromium workers

Hanaoka, Tomoyuki; Yamano, Yuko; Katsuno, Naochika; Kagawa, Jun; Ishizu, Sumiko

doi:10.5271/sjweh.176

Cited by 17 publications

(4 citation statements)

References 20 publications

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“…It was demonstrated that high p53 serum levels were identifiable several years prior to the cancer was able to be clinically detected (48). In a previous study by Hanaoka et al, high levels of serum pantropic p53 proteins were detected in workers that had a long history of Cr(VI) exposure, and thus were presumed to be at high risk for lung cancer (49). In the present study, we concluded that p53 expression was associated with the Cr(VI)-induced increase in DPC formation.…”

Section: Discussionsupporting

confidence: 64%

Time-order effects of vitamin C on hexavalent chromium-induced mitochondrial damage and DNA-protein crosslinks in cultured rat peripheral blood lymphocytes

Xiao

Chen

Luo

et al. 2013

Molecular Medicine Reports

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Hexavalent chromium [Cr(VI)] and its compounds have extensive applications in many industries and are widely known to cause occupational diseases as well as carcinogenic effects in humans. Mitochondrial damage, which is important in Cr(VI)‑induced cytotoxicity, may be characterized by the opening status of the permeability transition pore, the maintenance of the mitochondrial membrane potential and the level of malondialdehyde. The formation of DNA‑protein crosslinks (DPCs) in target tissues appears to be the direct and primary genotoxic effect of Cr(VI) exposure, and the lymphocytic DPCs may be viewed as a biomarker of internal Cr(VI) accumulation. It is well known that vitamin C (vit C) is an important biological reducing agent in humans and animals, which is capable of reducing Cr(VI). Regardless of the evidence from cell culture and in vivo experiments of the protective effect of the antioxidant, vit C, following exposure to Cr(VI), no studies have been conducted to date to demonstrate the time‑order effects of vit C on Cr(VI)‑induced mitochondrial damage and DPC formation. In the present study, by using peripheral blood lymphocytes from Sprague‑Dawley rats, we demonstrated that vit C pre‑ and co‑treatment have a protective effect against Cr(VI)‑induced loss of cell viability and mitochondrial damage, while only vit C co‑treatment has a protective effect against the Cr(VI)‑induced increase in DPCs. The mechanistic investigation revealed that cellular reactive oxygen species levels are correlated with Cr(VI)‑induced mitochondrial damage, and that p53 expression is correlated with the Cr(VI)‑induced increase in DPCs. We concluded that vit C exerts different time‑order effects on Cr(VI)‑induced mitochondrial damage and DPC formation, and that biomarkers, including DPC and p53, may be used in the assessment of the development of Cr(VI)‑induced cancer. These findings facilitate more detailed follow‑up of the Cr(VI)‑exposure populations for secondary prevention.

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Section: Discussionsupporting

confidence: 64%

Time-order effects of vitamin C on hexavalent chromium-induced mitochondrial damage and DNA-protein crosslinks in cultured rat peripheral blood lymphocytes

Xiao

Chen

Luo

et al. 2013

Molecular Medicine Reports

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“…Moreover, studying such cancer-related genes may help in the early detection, prevention, and the prognosis and outcome prediction of lung cancer [Nanus et al, 1990;Hanaoka et al, 1997;Wang et al, 1999].…”

Section: Discussionmentioning

confidence: 99%

Occupational cancer genetics: infrequent ras oncogenes point mutations in lung cancer samples from chromate workers

Ewis

Kondo

Lee

et al. 2001

American J Industrial Med

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“…High levels of serum pantropic p53 proteins have also been associated with high risk for lung cancer in workers after a long duration of exposure to chromium compounds (Hanaoka et al, 1997). The authors suggested that this would be an early effect marker and a possible indicator of cancer risk.…”

Section: Discussionmentioning

confidence: 99%

p53 protein, EGF receptor, and anti-p53 antibodies in serum from patients with occupationally derived lung cancer

et al. 1999

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The oncogene product epidermal growth factor receptor (EGF-R), the tumour suppressor gene product p53 and anti-p53 antibodies are detectable in the serum of certain cancer patients. Increased levels of some of these products were reported in lung cancer patients after occupational asbestos exposure and after exposure to polycyclic aromatic hydrocarbons or vinylchloride. In the first step, this study investigated the possible diagnostic value of serum EGF-R, p53-protein and anti-p53 antibodies, measured by an enzyme-linked immunosorbent assay, in lung tumour patients. In addition to being investigated on a molecular epidemiological basis, these parameters were examined as biomarkers of carcinogenesis, especially with regard to asbestos incorporation effects or of radon-induced lung cancers. Also, a possible effect of cigarette smoking and age dependence were studied. A total of 116 male patients with lung or pleural tumours were examined. The histological classification was four small-cell cancers, six large-cell cancers, 32 adenocarcinomas, 47 squamous carcinomas, 12 mixed lung carcinomas, five diffuse malignant mesotheliomas and ten lung metastasis of extrapulmonary tumours. Twenty-two lung cancers and all mesotheliomas were related to asbestos, 22 lung cancers were related to ionizing radiation and 61 patients had cigarette smoke-related lung cancer. Besides these patients 50 male patients with non-malignant lung or pleural diseases were included; of the latter eight subjects suffered from asbestosis. Controls were 129 male subjects without any lung disease. No significantly elevated or decreased serum values for p53 protein, EGF-R, or anti-p53 antibodies as a function of histological tumour type, age, or degree and type of exposure (asbestos, smoking, ionizing radiation) could be found. The utility of p53-protein, EGF-R and anti-p53 antibodies as routine biomarkers for screening occupationally derived lung cancers is limited. © 1999 Cancer Research Campaign

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Elevated serum levels of pantropic p53 proteins in chromium workers

Cited by 17 publications

References 20 publications

Time-order effects of vitamin C on hexavalent chromium-induced mitochondrial damage and DNA-protein crosslinks in cultured rat peripheral blood lymphocytes

Time-order effects of vitamin C on hexavalent chromium-induced mitochondrial damage and DNA-protein crosslinks in cultured rat peripheral blood lymphocytes

Occupational cancer genetics: infrequent ras oncogenes point mutations in lung cancer samples from chromate workers

p53 protein, EGF receptor, and anti-p53 antibodies in serum from patients with occupationally derived lung cancer

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