Elevated temperature incubation of enrichment media for the isolation of salmonellas from heavily contaminated materials

Abstract

SummaryIn three separate series, samples were examined for salmonellas by culture in selenite F broths incubated at 37 and 43° C. The samples used were:(1) Gauze swabs placed in sewage.(2) Gauze swabs placed in drains in abattoirs.(3) Sewage-polluted river water.In each series the higher temperature gave better results.The modification of tetrathionate broth for incubation at 43° C. and the adjustment of the incubation temperature to suit more inhibitory enrichment broths is discussed. The medium of Rappaport, Konforti & Navon (1956) is not suited to incubation at 43° C.We should like to acknowledge the help of Prof. Scott Thomson in the preparation of this paper, and the capable technical assistance of Mr T. R. Liddington and Mr John Morgan. We are also indebted to Dr H. Campbell, Senior Lecturer in Medical Statistics in the Welsh National School of Medicine, for his advice and to Mr M. C. Finniear, Deputy Public Analyst of the Glamorgan County Public Health Laboratory, for the calculation of molar tetrathionate in Rolfe's tetrathionate broth.

Elevated temperature technique for the isolation of salmonellas from sewage and human faeces

“…The enrichment of swab cultures at 410 C. was found to be appropriate for the isolation of salmonellas from sewage. This is in agreement with reports by other workers (Spino, 1966;Harvey & Price, 1968;Morahan & Hawksworth, 1969).…”

Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

“…There are, therefore, a minimum of six possible choices of tetrathionate broth available. Recently, a European group of microbiologists (Edel & Kampelmacher, 1969) produced evidence that commercial Muller-Kauffmann tetrathionate broth (Kauffmann, 1930(Kauffmann, , 1935) functioned well at the incubation temperature of 430 C. (Harvey & Thomson, 1953;Harvey & Price, 1968). The method has become the basis of a standard recommended technique.…”

Salmonella isolations in abattoirs in Greece

“…In these studies different enrichment and selective media were used but the incubation temperature was usually 370 C. (Jones, Bennet & Ellis, 1961;Harvey & Phillips, 1961; Valette, 1961;Report, 1964; Papadakis, 1965; Papadakis, 1968; and others). Harvey & Thompson (1953), however, who used selenite F broth, reported a better yield of salmonellas, especially from river water and sewage, when the enrichment medium was incubated at 430 C. Since then, this incubation temperature has been used with profit by some authors (Georgala & Boothroyd, 1964;Harvey & Price, 1968;Harvey, Price, Foster & Griffiths, 1969). The advantage of incubating the enrichment media at 430 C. was also evident in a recent comparative study of Salmonella isolations from faeces and minced meat in eight European laboratories (Edel & Kampelmacher, 1968).…”