Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Morgan

1977

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

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Salmonella surveillance with reference to pigs – Cardiff abattoir, 1968–1975

Morgan

1977

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“…Under these circumstances we would prefer selenite and/or tetrathionate which can accept large amounts of potentially infected material. The use of two enrichment media has certain advantages as each may have a bias towards isolation of certain serotypes (Harvey & Price, 1976). In the current series of observations recorded in Table 4, no useful comment on serotype bias can be made.…”

Section: Resultsmentioning

confidence: 99%

Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water

1981

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SUMMARYSix hundred and eighty three samples of chicken giblets were examined for salmonellas. Three hundred and forty nine of these were neck and crop specimens and 334 were combined liver and heart samples. Two hundred and ten, in all, contained salmonellas.The technique of examination included pre-enrichment in buffered peptone water at 37°C for 18 h and subculture to three enrichment media: MullerKauffmann tetrathionate, selenite F and Rappaport's magnesium chloride malachite green broth. Inocula from buffered peptone water to 10 ml of tetrathionate and selenite were 1 ml in each case. The inoculum from the pre-enrichment medium to 10 ml of Rappaport was 0 005 ml. Tetrathionate and selenite were incubated at 43°C for 48 h. Rappaport's medium was incubated at 37°C for 48 h. Subcultures from all three enrichment broths were made at 24 h and 48 h to brilliant green MacConkey agar. Selective agars were incubated at 37°C for 24 h.The most successful technique for salmonella isolation used Rappaport's medium, which was significantly more efficient than either tetrathionate or selenite. This finding reinforces results obtained using sewage polluted natural water as test material and it is suggested that routine examination of environment samples for salmonellas could be based on Rappaport's medium alone.If S. typhi, S. dublin or subgenus III salmonellas were likely to be present in the sample, the technique described here would require modification.

“…There is also the theoretical possibility that certain serotypes may be recovered more easily from one of the enrichment media rather than from another. We have demonstrated this with sewage polluted water (Harvey & Price, 1976). Koopman & Janssen (1973), however, in a study of reptile faeces found no definite preference for an enrichment method by certain serotypes.…”

Section: C3mentioning

confidence: 78%

Salmonella isolation from reptilian faeces: A discussion of appropriate cultural techniques

1983

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SUMMARYA short study of salmonella isolation from reptile faeces is described. The samples came from the reptile house at the Bristol Zoological Gardens. The wide range of salmonella serotypes present in the material, including representatives of all four subgenera, is noted. The important factors in the technique of isolation are discussed. These are choice of inoculum, enrichment medium, and plating medium, use of multiple subculture and serological isolation. If the number of samples available is scanty, an extended technique will probably yield more information than a simple method.