Elevation of ST6Gal I Activity in Malignant and Transitional Tissue in Human Colorectal Cancer

Vázquez-Martín, Cristina; Gil‐Martín, Emilio; Fernández-Briera, Almudena

doi:10.1159/000089999

Cited by 12 publications

(7 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mostafapour & Goldstein [26] reported that tumour cells with high metastatic potential tend to file a high ST6Gal I activity. Previous studies from our group [23] detected differences between enzyme activity of healthy tissue and transitional ones, in a reduced population of patients. If this difference is confirmed in subsequent analyses, it might seem that tissue cells suffer transitional changes of enzyme activity, perhaps as a prior episode for tumour processing.…”

Section: Discussionmentioning

confidence: 83%

“…[23]. The reaction mixture, in a total volume of 100 μL, contained: NaF 4 mM, MnCl 2 5 mM, Triton X-100 0.2% (v/v), MES buffer 40 mM (pH 6), 50 μg enzyme solution, 0.9 mg AST and 200 μM CMP-NeuAc [1 μM CMP- 14 C-NeuAc (specific activity 9.2-12 GBq/mmol, 77.4-80.32 μM, Amersham Biosciences, Uppsala, Sweden) and 199 μM CMP-NeuAc (Sigma, St. Louis, MO, USA)] as donor substrate.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and expression of CDw75 antigen in human colorectal cancer

et al. 2009

Self Cite

View full text Add to dashboard Cite

BackgroundIncreased ST6Gal I activity has been associated with the α(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC). Siaα(2,6)Galβ(1,4)GlcNAc sequence, known as CDw75, is a sialylated carbohydrate determinant generated by the ST6Gal I. This epitope has been reported to be associated with the progression of gastric and colorectal tumours, hence there are only a few conclusive studies to date.MethodsBy radioisotopic techniques we evaluated the ST6Gal I activity in healthy, transitional and tumour tissues from 43 patients with CRC. By immunohistochemistry we assessed the CDw75 expression in 25 colorectal adenomas, 43 tumours, 13 transitional and 28 healthy tissues of CRC patients.ResultsST6Gal I activity was likewise found to be statistically higher in tumour tissue respect to healthy tissue from CRC patients. CDw75 expression was positive in 20% of colorectal adenomas. Furthermore, 70% of tumour specimens and 8.3% of transitional specimens were positive for CDw75 expression, whereas none of the healthy ones showed the presence of the epitope.ConclusionThe major contribution of this study is the inclusion of data from transitional tissue and the analysis of CDw75 antigen expression in CRC and in colorectal adenomas, little known so far. ST6Gal I activity and CDw75 antigen expression were increased in CRC. Although their comparison did not reach the statistical significance, a great extent of patients showed both, an enhanced tumour ST6Gal I activity and an increased CDw75 expression in the tumour tissue. So, these two variables may play a role in malignant transformation. The expression of CDw75 in colorectal adenomas suggests that this antigen may be a tumour marker in CRC.

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Synthesis and expression of CDw75 antigen in human colorectal cancer

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…In colon cancer ST6Gal I and ST3Gal III increased their expression in carcinoma specimens [15]. ST3Gal III was prominently increased in cancer tissues compared with non-malignant colorectal mucosa [16] and an elevation of ST6Gal I activity was observed in malignant and transitional tissue [17]. In gastric cancer, high levels of ST3Gal III a in the tumour tissue correlated with secondary tumour recurrence [18].…”

Section: Introductionmentioning

confidence: 98%

α2,3-Sialyltransferase ST3Gal III Modulates Pancreatic Cancer Cell Motility and Adhesion In Vitro and Enhances Its Metastatic Potential In Vivo

et al. 2010

View full text Add to dashboard Cite

BackgroundCell surface sialylation is emerging as an important feature of cancer cell metastasis. Sialyltransferase expression has been reported to be altered in tumours and may account for the formation of sialylated tumour antigens. We have focused on the influence of alpha-2,3-sialyltransferase ST3Gal III in key steps of the pancreatic tumorigenic process.Methodology/Principal FindingsST3Gal III overexpressing pancreatic adenocarcinoma cell lines Capan-1 and MDAPanc-28 were generated. They showed an increase of the tumour associated antigen sialyl-Lewisx. The transfectants' E-selectin binding capacity was proportional to cell surface sialyl-Lewisx levels. Cellular migration positively correlated with ST3Gal III and sialyl-Lewisx levels. Moreover, intrasplenic injection of the ST3Gal III transfected cells into athymic nude mice showed a decrease in survival and higher metastasis formation when compared to the mock cells.ConclusionIn summary, the overexpression of ST3Gal III in these pancreatic adenocarcinoma cell lines underlines the role of this enzyme and its product in key steps of tumour progression such as adhesion, migration and metastasis formation.

show abstract

“…ST6Gal I activity is also associated with the acquisition of the neoplastic phenotype (15), as well as the resistance to irinotecan (16) and Fas-mediated apoptosis (17). Furthermore, in CRC patients, ST6Gal I activity is greater in tumor than in healthy tissue (18,19), particularly in metastatic tumors (20). The CDw75 antigen is expressed in the majority of colorectal tumors, where its expression is augmented with regard to the healthy mucosa (21,22).…”

Section: Introductionmentioning

confidence: 99%

Identification of proteins with the CDw75 epitope in human colorectal cancer

2017

View full text Add to dashboard Cite

Abstract. The CDw75 epitope is an α(2,6) sialylated antigen overexpressed in colorectal cancer (CRC), where its expression correlates with the progression of the disease. The CDw75 epitope is located mainly in N-glycoproteins, whose identity remains unknown. The aim of the present study was to identify proteins with the CDw75 epitope as a strategy to deepen the understanding of molecular pathogenesis of CRC and to identify novel biomarkers for this disease. For this purpose, a two-dimensional electrophoresis approach was employed. Protein spots in the gels were matched to the corresponding CDw75 positive spots in the immunoblotted polyvinylidene difluoride membranes, and further identification of the protein species was performed by mass spectrometry. Additionally, one-dimensional western blotting experiments were performed to verify the expression of these candidate proteins in the colorectal tissue and their coincidence in molecular mass with the CDw75-positive bands. The findings of the present study indicate that haptoglobin and the keratins 8 (K8) and 18 (K18) are proteins with the CDw75 epitope in the colorectal tissue from CRC patients and also suggest novel functions and cellular locations for these proteins in the colorectal tissue and in relation to CRC.

show abstract

Elevation of ST6Gal I Activity in Malignant and Transitional Tissue in Human Colorectal Cancer

Cited by 12 publications

References 55 publications

Synthesis and expression of CDw75 antigen in human colorectal cancer

Synthesis and expression of CDw75 antigen in human colorectal cancer

α2,3-Sialyltransferase ST3Gal III Modulates Pancreatic Cancer Cell Motility and Adhesion In Vitro and Enhances Its Metastatic Potential In Vivo

Identification of proteins with the CDw75 epitope in human colorectal cancer

Contact Info

Product

Resources

About