Elicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate

Abstract

The influence of methyl jasmonate on anthocyanin accumulation in roots of Kalanchoe blossfediana plants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l -1 , substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants of K. blossfeldiana than in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of old K. blossfeldiana plants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.

Exposure to Light of the Abaxial versus Adaxial Side of Detached Kalanchoë blossfeldiana Leaves Affects Anthocyanin Content and Composition Differently

“…The results obtained by contemporary methods only to a small extent confirm the results of Neyland et al [ 40 ], who detected in the leaves of this plant at the flowering stage the presence of only cyanidin-3-glucoside as the main anthocyanin and small contents of cyanidin-3,5-diglucoside. Previously, in the roots of K. blossfeldiana , eight anthocyanins were found, and quantitatively, the main one was cyanidin 3,5-di-glucoside [ 43 ].…”

“…However, when the leaves were removed, almost no anthocyanin formation was observed. It was later found that JA-Me substantially increased anthocyanin content in the roots of intact K. blossfeldiana plants under natural light conditions [ 43 ]. This means that distinct organs of K. blossfeldiana show varied responses to the action of JA-Me.…”

“…Saniewski et al [ 42 ] reported that methyl jasmonate (JA-Me) applied in lanolin paste stimulated the formation and accumulation of anthocyanins in the shoots of young K. blossfeldiana plants. Additionally, Góraj-Koniarska et al [ 43 ] demonstrated that JA-Me also stimulated anthocyanin accumulation in K. blossfeldiana roots that were exposed to natural light in the greenhouse. Subsequently, our previous study showed that in detached K. blossfeldiana leaves that were kept in an inverted position for five days, higher anthocyanin accumulation occurred on the abaxial side [ 44 ].…”

Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants

“…These compounds are associated with flower pigmentation of other species belonging to the Kalanchoe genus, together with other anthocyanins, such as peonidin and delphinidin (Nielsen et al, 2005). However, they have also been produced in root cultures (Góraj-Koniarska et al, 2015). The presence of anthocyanins in the leaves of Bryophyllum spp.…”

10th International Conference, Plant Functioning Under Environmental Stress, September 16-19, 2015, Cracow, Poland

“…At the same time increased the quantum ef ciency of energy trapping in PSII and ef ciency index was accompanied by decrease in energy dissipation suggesting more effective energy usage. Methyl jasmonate (JA-Me) is an elicitor that causes the formation of anthocyanins in roots of Kalanchoe blossfeldiana (Góraj et al, 2014;Góraj-Koniarska et al, 2015). The anthocyanins are accumulated in roots of intact plants, but not in excised roots.…”