Elicitation studies in cell suspension cultures of Cannabis sativa L.

Flores-Sánchez, Isvett Josefina; Peč, Jaroslav; Fei, Junni; Choi, Young Hae; Dušek, Jaromír; Verpoorte, Robert

doi:10.1016/j.jbiotec.2009.05.006

Cited by 64 publications

(39 citation statements)

References 88 publications

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“…As indicated by OPLS-DA and O2PLS results, sugar levels decreased by time; sucrose levels were significantly lower at 24 and 72 h in the JAelicited cells compared to the control (P \ 0.05). In contrast, Flores-Sanchez et al (2009) found no significant difference of sucrose and glucose levels in methyl jasmonate-elicited Cannabis sativa cell suspension cultures if compared to their respective controls; on the other hand, the elicitation treatment resulted in increased levels of fumaric acid in the C. sativa cells. In the C. roseus cells, some components of the tricarboxylic acid (TCA) cycle; succinic acid, fumaric acid, and malic acid were increased at 24 and 72 h after elicitation, though only malic acid levels were statistically significant compared to the control (P \ 0.05).…”

Section: Relative Levels Of Metabolites Detected By Nmrcontrasting

confidence: 56%

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Saiman

Mustafa

Choi

et al. 2015

Plant Cell Tiss Organ Cult

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In Catharanthus roseus cell cultures, the terpenoid moiety is considered as the limiting factor in terpenoid indole alkaloid (TIA) production. The pathway of terpenoidal precursors in TIA is strongly linked with other terpenoid pathways, suggesting that TIA production might be limited by competition for the five-carbon (C5) precursors. This raises the question whether the stimulation of TIA production by certain signal molecules is due to a redistribution of C5 precursors between the associated terpenoid pathways and/or to a total increase of the precursor availability. To investigate the effect of a TIA-increasing signal molecule on C5 distribution, the cell suspension cultures of C. roseus were elicited with jasmonic acid (JA) and the metabolic changes of different terpenoid pathways were evaluated targeting on TIA (monoterpenoid; C10), carotenoids (tetraterpenoid; C40), and phytosterols (triterpenoid; C30). The chromatographic analyses showed that TIA and carotenoid levels almost doubled upon JA elicitation, while phytosterol levels remained constant if compared to the controls. Apparently, both TIA and carotenoid routes benefit from an increased C5 flow in the methyl-erythritol phosphate pathway, and potential export of C5 precursors to the cytosolic terpenoid routes, e.g., towards the phytosterols is minimal. However, the relative composition of individual compounds within each group remains similar when comparing elicited and control cells. This suggests that the increased production of TIA and carotenoid upon JA elicitation is predominantly caused by an increase of the precursor availability rather than due to a redistribution of C5 precursors between the associated terpenoid pathways. Furthermore, NMR-based metabolomics analysis showed a discrimination of JAelicited and control cells between 24 and 72 h after treatments with significant changes in levels of strictosidine, malic acid, and sucrose. This study portrays metabolic alterations upon JA elicitation and channeling of C5 precursors in different terpenoid biosynthetic pathways, which provides a knowledge platform for developing strategies to engineer fluxes in a complex biosynthetic network in order to obtain high TIA-producing C. roseus cell lines.

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Section: Relative Levels Of Metabolites Detected By Nmrcontrasting

confidence: 56%

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Saiman

Mustafa

Choi

et al. 2015

Plant Cell Tiss Organ Cult

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“…Principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) were performed with the SIMCA-P software (v. 11.0, Umetrics Umeå, Sweden). Compounds were quantified by the relative ratio of the intensities of their peak-integrals and the ones of internal standard according to Flores-Sanchez et al (2009). The characteristic 1 H-chemical shifts (d) and coupling constant (Hz) of identified metabolites were previously reported (Flores-Sanchez et al 2009).…”

Section: Nmr Measurements and Data Analysesmentioning

confidence: 99%

“…For example, in plant cell cultures and plants treated with biotic elicitors clear qualitative and quantitative variations in the metabolic pool were shown (Sanchez-Sampedro et al 2007;Jahangir et al 2008). Although, cannabis cell cultures do not produce cannabinoids, even after elicitation treatments (Flores-Sanchez et al 2009), analyses in the content of the metabolite pool from two cell lines were made in order to study the effect of elicitation on the Cannabis sativa cell suspension culture metabolome. Here, we report the results from these studies.…”

Section: Introductionmentioning

confidence: 99%

Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by 1H-NMR spectroscopy

et al. 2010

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Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with jasmonic acid (JA) and pectin as elicitors to evaluate their effect on metabolism from two cell lines using NMR spectroscopy and multivariate data analysis. According to principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA), the chloroform extract of the pectin-treated cultures were more different than control and JA-treated cultures; but in the methanol/water extract the metabolome of the JA-treated cells showed clear differences with control and pectin-treated cultures. Tyrosol, an antioxidant metabolite, was detected in cannabis cell cultures. The tyrosol content increased after eliciting with JA.

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“…The results indicated that methyl jasmonate increased production of β -amyrin and saponins, while yeast extract induced accumulation of shikimic acid, an early precursor in the prenylpropanoid pathway, and ultraviolet light gave insignifi cant effects [55] . However not all elicitations can stimulate targeted metabolite production, such as elicitation in cell suspension cultures of C. sativa L., which did not fulfi ll the expectation of a stimulation of cannabinoids production [56] .…”

Section: Characterization Of Metabolites In Plant Cultures and Transgmentioning

confidence: 96%

Metabolomics as a Bioanalytical Tool for Characterization of Medicinal Plants and Their Phytomedical Preparations

Happyana

Muntendam

Kayser³

2012

Pharmaceutical Biotechnology

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Elicitation studies in cell suspension cultures of Cannabis sativa L.

Cited by 64 publications

References 88 publications

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by 1H-NMR spectroscopy

Metabolomics as a Bioanalytical Tool for Characterization of Medicinal Plants and Their Phytomedical Preparations

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