Elimination of multicopy plasmid R6K by bleomycin

Attfield, Paul V.; Pinney, R. J.

doi:10.1128/aac.27.6.985

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…It is believed bleomycin chelate metal ions (primarily iron) producing a pseudo enzyme that reacts with oxygen to produce superoxide and hydroxide free radicles that cleave DNA [29]. Our results coincide with [30] who used bleomycin to eliminate multi copy plasmid R6K from growing cells of E. coli AB1157. Measurement of R6K-encoded β-lactamase and covalently closed plasmid DNA indicated that the drug causes a progressive reduction in plasmid copy number.…”

Section: By Bleomycinsupporting

confidence: 86%

Plasmid Profile, Curing Analysis, and antibacterial Activity of Alcea arebellensis plant Against Multidrug Resistance Staphylococcus aureus

Abdulrahman¹,

Kh.M.Barzani²,

Rasheed³

2023

Tikrit J. Pure Sci.

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A total of 50 Staphylococcus aureus isolates were identified from burn , otitis media, wound and urine infection from patient admitted to the central lab and internal lab of teaching hospital and Rizgary hospital in Erbil city during the period 10thNovember 2011 to 30th March 2012. All the isolate identified according to Microscopical, cultural and biochemical tests. S. aureus tested for antibiotics susceptibility test using agar diffusion method .The antibiotics susceptibility testing showed that 49(98%) , 48(96%), 48(94%), 9(18%),8(10%), 7(14%), and 4(8%), were resistant to chloramphenicol , Amikacin, ampicillin, Vancomycin ,Cefotaxime, Cefazolin ,and ceftriaxone but all the isolates were sensitive to amoxicillin. Also the results showed that isolate no.38 was resistant to seven antibiotics under study which contain five plasmids with molecular weight more than 10 kbp. The results also revealed that 5(10%), 25(50%), and 20(40%) of S.aureus were reported to produce alpha, beta, gamma hemolysis respectively. Results of plasmid curing experiments revealed that elevated temperature at 46oC were completely remove the plasmid that harbor antibiotic resistance gene for the antibiotic understudy while for bleomycin at 50µg/ml had lower effect. The antibacterial activity of aqueous and alcoholic extract of flowers and leaves of the Alcea arebelensis was studied against some resistant isolates of S. aureus, the results showed the inhibitory effect of plant extracts against selected isolates except the alcohol leaf extract which showed no inhibitory activity against all studied bacterial isolates.

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Section: By Bleomycinsupporting

confidence: 86%

Plasmid Profile, Curing Analysis, and antibacterial Activity of Alcea arebellensis plant Against Multidrug Resistance Staphylococcus aureus

Abdulrahman¹,

Kh.M.Barzani²,

Rasheed³

2023

Tikrit J. Pure Sci.

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“…Rotimi and Duerden (1982) reported action of acridine dye on E.coli R + and Bacteroides fragillis cells leading to complete loss of Rfactor. Pan et al (1981), Attfield and Pinney (1985) used mytomicin C and bleomycin treatment for curing multicopy plasmid. The relation of plasmid in development resistance was studied by Pan et al (1981) in Enterobacter aerogenes against mercury.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of ciprofloxacin resistance in Shigella dysenteriae

Lankeshwar¹,

Bagde²

2013

Afr. J. Biotechnol.

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Bacterial resistance to quinolones has emerged rapidly and such resistance has traditionally been attributed to the chromosomally mediated mechanisms that alter the quinolone targets and/or overproduce multidrug resistance efflux pumps. In the present investigation possible mechanism of ciprofloxacin (Cp) resistance in Shigella dysenteriae was studied. While the growth of sensitive S. dysenteriae was completely inhibited at 1 µg /ml concentration of ciprofloxacin, the resistant strain tolerated even 10 µg /ml concentration of ciprofloxacin. Mechanism of resistance was found to be the presence of plasmid.

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“…In addition to the above mechanism, it has been reported that copy number and mode of replication control of particular plasmids may play an important role as to whether a plasmid can be cured or not (Attfield et a/., 1985;Danilevskaya et at., 1980). This might be the reason for failure to eliminate R-factors from some of the E. colistrains.…”

Section: Discussionmentioning

confidence: 99%

Plasmid elimination from clinical isolates of Echerichia coli by ciprofloxacin and other inhibitors of DNA gyrase

et al. 1994

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The susceptibility of 50 drug resistant strains of Escherichia co/i of human gut was determined against ciprofloxacin, acridine orange (AO) and sodium dodecyl sulphate (SDS). Curing efficacy of these agents were worked out at subminimal inhibitory concentrations. Ciprofloaxacin was found a better curing agent for E co/i R-plasmids, eliminating R-factors from 48% of the strains followed by SDS and A0 which eliminated 24% and 20% of the drug resistance deteminants, respcetively. Elimination of R-plasmids was found dependent on the concentrations of curing agents and nature of R-plasmids.

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Elimination of multicopy plasmid R6K by bleomycin

Cited by 6 publications

References 24 publications

Plasmid Profile, Curing Analysis, and antibacterial Activity of Alcea arebellensis plant Against Multidrug Resistance Staphylococcus aureus

Plasmid Profile, Curing Analysis, and antibacterial Activity of Alcea arebellensis plant Against Multidrug Resistance Staphylococcus aureus

Mechanism of ciprofloxacin resistance in Shigella dysenteriae

Plasmid elimination from clinical isolates of Echerichia coli by ciprofloxacin and other inhibitors of DNA gyrase

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