Elimination of ornithogalum mosaic virus in the Ornithogalum cv. Rogel through meristem tip culture and chemotherapy

Vcelar, Bela M.; Ferreira, D. I.; Niederwieser, J. G.

doi:10.1007/bf00036146

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Acyclovir had no effect on Potato virus S (Park et al 1994) and on Ornithogalum mosaic potyvirus (Vcelar et al 1992). Caner et al (1985) showed its inhibitory effect on Bean golden mosaic virus infecting Phaseolus lunatus when sprayed on the preinoculated plants.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 95%

Indexing and production of virus-free chrysanthemums

Ram¹,

Verma²,

Singh³

et al. 2005

Biologia plant.

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Chrysanthemum morifolium cv. Regol Time plants were found to be infected with Chrysanthemum B carlavirus (CVB). They were made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The maximum number of virus-free plants (26.7 %, as indexed by RT-PCR) was obtained with 2-thiouracil at 0.04 g dm -3 concentration. Only 12 % plants were found to be virus-free, after being kept at 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR was found to be the most reliable method.Additional key words: antiviral compounds, Chrysanthemum B carlavirus, Chrysanthemum morifolium, DAS-ELISA, in vitro production, RT-PCR.

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Section: ⎯⎯⎯⎯mentioning

confidence: 95%

Indexing and production of virus-free chrysanthemums

Ram¹,

Verma²,

Singh³

et al. 2005

Biologia plant.

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“…Although thermotherapy has been applied to eliminate LSV effectively in L. × elegans (Nesi et al, 2009), there has been no report on the successful elimination of CMV and LMoV using thermotherapy; therefore, it seems that meristematic tip culture and either meristematic tip culture or chemotherapy should be used for LMoV and CMV elimination, respectively, and meristematic tip culture, chemotherapy or thermotherapy can eradicate LSV. Multiplication in vitro, acclimation, and cultivation in a phytotron It has been reported that the virus infection rate increased in acclimated plantlets in many bulbous plants (Phillips, 1990;Vcelar et al, 1992;Xu and Niimi, 1999;Xu et al, 2000). In this study, to check for virus propagation after acclimation, virus-free bulblets produced by meristematic tip culture with DHT were grown in the phytotron and examined by RT-PCR virus detection methods.…”

Section: Second Meristematic Tip Culturementioning

confidence: 99%

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

Masuda

Thien

Hải

et al. 2011

J. Japan. Soc. Hort. Sci.

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Lilium brownii var. colchesteri has unique and ornamental floral characteristics in graceful harmony with flower and anther colors, flower shape, elegant fragrance, and flower color change from yellowish cream to white during anthesis. There are, however, few accessions conserved in Japan up to the present, and they often show abnormally shaped flowers and mosaic leaves seemingly due to virus infection. Virus-free bulblets were established by combining meristematic tip culture and chemotherapy. At initial diagnosis with RT-PCR, Cucumber mosaic virus (CMV), Lily mottle virus (LMoV) and Lily symptomless virus (LSV) were detected from leaf tissues of the mother plants. All regenerated bulblets obtained by meristematic tip culture of the mother plants were still infected by at least one of the viruses. The meristematic tip of the bulblets infected with either LSV or LMoV was selected for subsequent culture with 2,4-dioxohexahydro-1,3,5-triazine (DHT), an antiviral chemical. LSV was eliminated successfully in mericlones from bulblets with LSV, whereas LMoV was not from those with LMoV. The virusfree bulblets were transferred to new medium without DHT, and multiplied by in vitro scaling. They were then acclimated in a phytotron glass room at 20°C. The plants were confirmed to be virus-free after 18-months' acclimation. It was concluded that the combination of meristem tip culture and chemotherapy is practical for producing virus-free plants of L. brownii var. colchesteri.

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“…This necessitates the production of certified healthy virus-free propagation material for establishing new orchards. Shoot tip grafting (STG), shoot tip/ meristem culture, thermotherapy, and chemotherapy were used separately or in combination to eliminate viruses from the plants (Vcelar et al 1992;Fletcher et al 1998;Manganaris et al 2003;Nascimento et al 2003;Valero et al 2003;Ram et al 2005;Verma et al 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting

Sharma

Rani

Zaidi

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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This paper reports the elimination of Indian citrus ringspot virus (ICRSV) from "kinnow" (Citrus nobilis Lour × Citrus deliciosa Tenora) employing phytotherapy coupled with shoot tip grafting under in vitro conditions. Nodal segments from infected mother plant (indexed by indirect enzyme-linked immunosorbent assay [ELISA] and reverse transcriptase PCR [RT-PCR]) were cultured on Murashige and Skoog medium containing 2iP (1 mg/l or 4.9 μM) and malt extract (800 mg/l) along with different concentrations of aqueous extracts from leaves of Azadirachta indica (Neem), Sorghum vulgare (Jowar), and roots of Boerhaavia diffusa (Punarnava). Shoot tips were excised from the nodal sprouts and grafted on to rough lemon (Citrus jambhiri) under aseptic conditions. Maximum effect (50% virus elimination) was seen for aqueous leaf extracts of A. indica followed by B. diffusa root extract (42.86%) and S. vulgare leaf extract (31.58%). Plants/plantlets were considered virus-free only when showing negative reactions by both indirect ELISA and RT-PCR.

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Elimination of ornithogalum mosaic virus in the Ornithogalum cv. Rogel through meristem tip culture and chemotherapy

Cited by 11 publications

References 10 publications

Indexing and production of virus-free chrysanthemums

Indexing and production of virus-free chrysanthemums

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting

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