ELISA-Based Detection of Soybean Proteins: A Comparative Study Using Antibodies Against Modified and Native Proteins

Cucu, Tatiana; Devreese, Bart; Kerkaert, Barbara; Rogge, Maarten; Vercruysse, Lieselot; Meulenaer, Bruno De

doi:10.1007/s12161-011-9341-y

Cited by 32 publications

(20 citation statements)

References 25 publications

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“…However, it has to be assured that defined antibodies raised in animals 313 against single allergen molecules are used. Previously, also antibodies raised against whole soybean 314 protein extract were applied to test for the occurrence of single allergens in ELISA (Cucu et al 2012). 315…”

Section: Identification Of Potential Soybean Allergens For Assessmentmentioning

confidence: 99%

Assessment of endogenous allergenicity of genetically modified plants exemplified by soybean – Where do we stand?

Selb

Wal

Moreno

et al. 2017

Food and Chemical Toxicology

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According to EU regulation, genetically modified (GM) plants considered to be allergenic have to be assessed concerning their endogenous allergens before placement on the EU market, in line with the international standards described in Codex Alimentarius. Under such premises, a quantitative relevant increase in allergens might occur in GM plants as an unintended effect compared with conventionally produced crops, which could pose a risk to consumers. Currently, data showing a connection between dose and allergic sensitisation are scarce since the pathophysiological mechanisms of sensitisation are insufficiently understood. In contrast, data on population dose-distribution relationships acquired by oral food challenge are available showing a connection between quantity of allergenic protein consumed and the population of allergic individuals experiencing reactions. Soybean is currently the only recognised allergenic GM food by law for which EFSA has received applications and was therefore taken as an example for defining an assessment strategy. Identification of potential allergens, methodology for quantification as well as risk assessment considerations, are discussed. A strategy is proposed for the identification, assessment and evaluation of potential hazards/risks concerning endogenous allergenicity in food derived from plants developed by biotechnology. This approach could be expanded to other allergenic foods in the future, whenever required.

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Section: Identification Of Potential Soybean Allergens For Assessmentmentioning

confidence: 99%

Assessment of endogenous allergenicity of genetically modified plants exemplified by soybean – Where do we stand?

Selb

Wal

Moreno

et al. 2017

Food and Chemical Toxicology

View full text Add to dashboard Cite

show abstract

“…Biosensors have helped in the development of a variety of DNA or protein detection formats that include fast and automated analyses (Poms et al 2004, van Hengel 2007, Pollet et al 2011, Alves et al 2015. To overcome the loss of target detectability, generating specific and sensitive antibodies against the native and processed allergens or their stable peptides (Cucu et al 2012(Cucu et al , 2013 is also of increased interest.…”

Section: Future Trendsmentioning

confidence: 99%

Food Allergy

Sathe

Liu

Zaffran

2016

Annu. Rev. Food Sci. Technol.

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Food allergy is receiving increased attention in recent years. Because there is currently no known cure for food allergy, avoiding the offending food is the best defense for sensitive individuals. Type I food allergy is mediated by food proteins, and thus, theoretically, any food protein is a potential allergen. Variability of an individual's immune system further complicates attempts to understand allergen-antibody interaction. In this article, we briefly review food allergy occurrence, prevalence, mechanisms, and detection. Efforts aimed at reducing/eliminating allergens through food processing are discussed. Future research needs are addressed.

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“…There were, however, no bands seen at the 50 kDa marker, and this may imply that the processing conditions (baking at 350 ∘ F) used in the preparation of the cookies modified these proteins. Cucu et al 25 made similar observations after incubating soy protein extract with glucose and sunflower oil at 70 ∘ C in order to mimic major protein changes that occur during the interaction of proteins with lipids and reducing sugars. They reported that the bands representing the 11S glycinin and the 7S -conglycinin lost intensity and attributed this to a combined effect of incubation with glucose as well as with sunflower oil.…”

Section: A B C D E F G H I J K L M N O P a B C D E F G H I J K L M N O Pmentioning

confidence: 79%

Evaluation of the efficiency of three extraction conditions for the immunochemical detection of allergenic soy proteins in different food matrices

Amponsah

Nayak

2017

J Sci Food Agric

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ELISA-Based Detection of Soybean Proteins: A Comparative Study Using Antibodies Against Modified and Native Proteins

Cited by 32 publications

References 25 publications

Assessment of endogenous allergenicity of genetically modified plants exemplified by soybean – Where do we stand?

Assessment of endogenous allergenicity of genetically modified plants exemplified by soybean – Where do we stand?

Food Allergy

Evaluation of the efficiency of three extraction conditions for the immunochemical detection of allergenic soy proteins in different food matrices

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