ELISA in the evaluation of therapeutic response to albendazole in neurocysticercosis

Husain, Nuzhat; Shukla, Neha; Kumar, Raj; Husain, Mazhar; Chaturvedi, Arun; Agarwal, Gaurav; Gupta, Rakesh K.

doi:10.1016/j.jinf.2007.09.014

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…Thirteen (81.2%) of 16 and 12 (80%) of 15 nonresponders continued to show high anti-cysticercus IgG and IgM titers, respectively. The study suggested that a negative ELISA result for both IgG and IgM antibodies denotes the cure of NCC [13]. In the present study, before the treatment, all of the serum samples positive with the CSE and LMM antigens were also positive with the use of ES antigens.…”

Section: Discussionsupporting

confidence: 53%

“…Very few reports indicate the evaluation of ELISA to detect humoral immune response in the assessment of therapeutic response after the treatment with the use of crude [12], purified, and heterologous antigens [13]. In the present study, with the use of crude antigen, out of 14 serum samples positive before the treatment, 5 (35.7%) samples were persistently seropositive even 3–6 months and 12 months PT.…”

Section: Discussionmentioning

confidence: 47%

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Pediatric Neurocysticercosis: Usefulness of Antibody Response in Cysticidal Treatment Follow-Up

Atluri

Gogulamudi

Singhi

et al. 2014

BioMed Research International

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Serum and urine samples were collected from 33 NCC patients before the albendazole treatment, 3–6 and 12 months PT. At 3 months PT, 24 (72.7%) patients had no detectable CT/MRI lesions and 9 (27.2%) patients had persistent lesions. Antibody response to crude soluble extract (CSE), excretory secretory (ES), and lower molecular mass (LMM) (10–30 KDa) antigenic fraction of T. solium cysticerci was detected in serum and urine samples by ELISA. Before the treatment, out of 33 NCC children, 14 (42.4%), 22 (66.6%), and 11 (33.3%) serum samples were found positive with the use of CSE, ES, and LMM antigen, respectively. At 3–6 months PT, positivity rate was 5 (15.1%), 2 (6%), and 4 (12.1%) and at 12 months PT, positivity rate was 5 (15.1%), 0, and 3 (9%) with the use of CSE, ES, and LMM antigen, respectively. There was no significant difference in the positivity with the use of three antigens in pretreatment and PT urine samples. The study suggests that the use of ES antigen to detect antibody in serum samples may serve better purpose to evaluate the therapeutic response in patients with NCC.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 47%

Pediatric Neurocysticercosis: Usefulness of Antibody Response in Cysticidal Treatment Follow-Up

Atluri

Gogulamudi

Singhi

et al. 2014

BioMed Research International

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“…When regarding the diagnostic approaches for NCC, the immunologic tests more commonly used to detect antibodies against T. solium are the enzyme-linked immunosorbent assay (ELISA) and Western Blot (WB) in the cerebrospinal fluid (CSF) and/or serum (Montero et al 2003;Barcelos et al 2005;Schantz 2006;Husain et al 2008). Using purified glycoproteins by enzyme-linked immunoelectrotransfer blot, Tsang et al (1989) found high sensitivity and specificity indexes in serum and CSF samples.…”

Section: Introductionmentioning

confidence: 99%

Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid

et al. 2009

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The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB) tests compared with the use of metacestodes antigen of Taenia solium in cerebrospinal fluid (CSF) samples. The samples were obtained from 35 patients with definitive neurocysticercosis (NCC)-group 1-and 44 patients with other neurological disorders (control)-group 2. The sensitivity and specificity of ELISA, using antigen obtained from T. solium, applied to the patients of group 1 yielded results of 100%. When the tests were conducted using T. saginata metacestodes, results were 100% and 93.2%, respectively. The 47-52-, 64-68-, and 70-kDa antigens showed high frequencies in CSF samples from patients with NCC when WB was conducted with both antigens. The results indicate that T. saginata metacestodes can be used as an alternative antigen for the diagnosis of human NCC in CSF samples.

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“…In many countries where T. solium is endemic, neuroimaging methods could be inaccessible and/or too expensive for the population at risk, often rural. Under these conditions, serology may provide a viable tool for diagnosis of the infection (6,16,18,35).As NC immunodiagnosis continues to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for the preparation of T. solium homologous antigen (33), alternative antigens, including heterologous antigens from Taenia saginata, have also been used with satisfactory results to diagnose human NC (29,31,32). T. saginata cysticerci in the cystic stage may be obtained in considerable amounts, because the eating habits in many countries, including Brazil, are based mainly on cattle meat consumption.…”

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confidence: 99%

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confidence: 99%

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

Oliveira

Machado

Mineo

et al. 2010

Clin Vaccine Immunol

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Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin-and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64-to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of T. saginata metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.Taenia solium is a parasite whose larvae (metacestodes) may be located in the central nervous system of humans, causing neurocysticercosis (NC). Clinical manifestations are nonspecific and vary in severity, ranging from headaches, dizziness, and occasional seizures to a very severe neurological condition with intracranial hypertension or dementia (39,40,42).The diagnosis of NC is given by the combined analysis of clinical data and neuroimaging (computerized tomography and magnetic resonance imaging) as well as immunological and epidemiological data (10). From the neuroimaging findings of NC, only the presence of cystic lesions demonstrating the scolex should be considered pathognomonic (4). In many countries where T. solium is endemic, neuroimaging methods could be inaccessible and/or too expensive for the population at risk, often rural. Under these conditions, serology may provide a viable tool for diagnosis of the infection (6,16,18,35).As NC immunodiagnosis continues to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for the preparation of T. solium homologous antigen (33), alternative antigens, including heterologous antigens from Taenia saginata, have also been used with satisfactory results to diagnose human NC (29,31,32). T. saginata cysticerci in the cysti...

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ELISA in the evaluation of therapeutic response to albendazole in neurocysticercosis

Cited by 6 publications

References 19 publications

Pediatric Neurocysticercosis: Usefulness of Antibody Response in Cysticidal Treatment Follow-Up

Pediatric Neurocysticercosis: Usefulness of Antibody Response in Cysticidal Treatment Follow-Up

Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

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