Ellagic acid in Emblica officinalis exerts anti-diabetic activity through the action on β-cells of pancreas

Fatima, Nighat; Hafizur, Rahman M.; Hameed, Abdul; Ahmed, Shakil; Nisar, Maliha; Kabir, Nurul

doi:10.1007/s00394-015-1103-y

Cited by 130 publications

(71 citation statements)

References 32 publications

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“…Caffeic acid esters are able to stimulate glucose transport in skeletal muscle via increasing GLUT4 translocation, reduce hepatocellular glucose production and adipogenesis in an AMPK-dependent manner 71 . Ellagic acid lowered significantly blood glucose level by stimulating insulin secretion 73 . Gallic acid and its derivatives play significant role through activation of the AMPK/Sirt/PGC1α pathway 74 .…”

Section: Discussionmentioning

confidence: 95%

LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

Balkrishna

Joshi

Das

et al. 2020

Preprint

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19The incidence rate of diabetes mellitus is increasing worldwide. Herbal formulations have 20 recently gained importance as an alternative therapeutic option in controlling diabetes without 21 causing any side effects. In the present study, we have demonstrated maintenance of glycemic 22 homeostasis and anti-adipogenic potential of a herbo-mineral formulation Divya MadhuKalp 23 (DMK). Initially, we evaluated the presence of bioactive compounds in DMK using LC/MS-24 QToF analysis. In-vitro analysis of DMK in L6 (skeletal muscle) cells showed a significant 25 increase in cellular glucose uptake. Similarly, a human equivalent dose of DMK significantly 26 reduced blood glucose level in normoglycemic and oral glucose tolerance rat model. DMK 27 extract also inhibited formation of advanced glycation end product and showed anti-α-28 glucosidase activity. Further analysis of DMK in 3T3 L1 pre-adipocytes demonstrated anti-29 adipogenic activity through reduction in intracellular lipid accumulation and triglyceride contents 30 along with downregulation of major adipogenic transcriptional factors (PPAR-γ and C/EBPα) 31 and, adipocytes marker genes (LPL, AP2 and adiponectin). In conclusion, DMK exhibited anti-32 diabetic and anti-adipogenic activities by synergistic effect of its bioactive compounds and can 33 be considered as a potent herbo-mineral formulation for treating metabolic diseases. 34 35 expression. 37 38 39 which may induce unwanted side-effects, natural herbal formulations tend to show similar 63 efficacy with minimal to no side-effects and cost effectiveness 6, 7, 8 . In traditional Indian 64 medicine system, numerous herbal and herbo-mineral formulations have been used for treating 65 various diseases, but their scientific evidence is still lacking. Divya MadhuKalp (DMK) is an 66Ayurvedic herbo-mineral formulation has been extensively used in clinical practices for 67 managing blood glucose levels during onset of DM. The DMK comprises of eight herbs and one 68 minerals-rich organic component in specific proportion to make complex bioactive formulation 69 (Table 1). The ingredients are; Momordica charantia 9 ; Picrorhiza kurroa 10 ; Swertia chiraita 11 ; 70Azadirachta indica 12 ; Trigonella foenum-graecum 13 ; Syzyium cuminii 14 ; Withania somnifera 15 ; 71Aconitum hetrophyllum 16 and Shilajeet (Asphaltum) 17,18 . The ingredients in DMK have been 72 used for diabetic therapy in ancient Indian Ayurvedic medicinal system. In order to explore the 73 pharmacological mechanism of poly herbal extracts, accurate characterization of the bioactive 74 compounds is very essential. Moreover, the quality control is very important factor in discovery 75 of a new drug. LC/MS-QToF technology is most appropriate analytical method for the full 76 characterization and quality control of poly herbal extract 19 . DMK associated herbal ingredients 77 have been found to be rich in secondary metabolite compositions such as, phenolics, terpenes, 78 and nitrogen containing compounds. These active bio-molecules play an important rol...

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Section: Discussionmentioning

confidence: 95%

LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

Balkrishna

Joshi

Das

et al. 2020

Preprint

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“…It stimulates basal glucose uptake, the primary requisite for maintaining glucose homeostasis, through LKB1 (Liver Kinase B1) mediated AMP-activated protein kinase (AMPK) activation and also possesses insulin-like properties [30, 31]. On the other hands, polyphenolic ellagic acid potentiates pancreatic secretion, increases glucose uptake, liver and muscle glycogen content, decreases glucose intolerance and reduces glucose absorption by inhibiting glucosidase enzyme [32, 33]. The flavonoid, kaempferol improves insulin-stimulated glucose uptake in mature adipocytes [34] and naringenin suppresses carbohydrate absorption from intestine, exerts extra-pancreatic action [35].…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and pharmacological activities of Pisum sativum

Zilani

Sultana

Rahman

et al. 2017

BMC Complement Altern Med

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BackgroundConsumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out.MethodsTotal polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay.ResultsTotal polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, β-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC50 value of 158.52 μg/mL in DPPH scavenging assay and also showed comparable reducing power.ConclusionAlong with other compounds ellagic acid and β-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine.

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“…EA acid lowered diabetes-associated triglyceride level, oxidative stress, exhibit anti-coagulatory and antiin lammatory protection in cardiac muscle cells of diabetic mice via increased cardiac mRNA expression like GPX1, SOD, and catalase in diabetic mice indicating that EA can be used for controlling Diabetes cardiac myopathy. (Chao et al, 2009;Fatima et al, 2017)documented that EA in Emblica Of ic-inalis exhibit antihyperglycemic activity in STZinduced non-obese type 2 diabetic rats via an act on pancreatic β-cells that enhances secretion of insulin and reduce glucose intolerance (Fatima et al, 2017).…”

Section: Antidiabetic Activity Of Ellagic Acidmentioning

confidence: 99%

“…In the oral glucose tolerance test, the oral administration of 3.5 mg CGA/kg BW has shown a reduction in the glycemic index of food by alleviating the intestine glucose absorption but did not signi icantly reduce the blood glucose. Bassoli et al (2008) is reported that chlorogenic acid can improve glucose and lipid metabolism in diabetes patients (Fatima et al, 2017).…”

Section: Antidiabetic Activity Of Chlorogenic Acidmentioning

confidence: 99%

Hybrid drug combination: A new treatment strategy for type 2 diabetes- A review

Sharma¹,

P²,

Mehdi³

et al. 2020

ijrps

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Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia arising from deregulation in insulin secretion, insulin action, or both. The current synthetic drugs have dose-dependent side effects which confined their uses. The phytochemicals are the natural compounds that have better therapeutic efficacy and interacts synergistically with oral hypoglycemic drugs. The addition of phytochemicals with OHDs may reduce the dose of synthetic drugs as well as their side effects and toxicity. A detailed outline about such combinations like Ferulic acid & THZ/Metformin, Ellagic acid & Pioglitazone (THZ), Chlorogenic acid & THZ/Metformin, Caffeic acid & THZ/Metformin, eugenol acid & THZ/Metformin, cinnamic acid & THZ/Metformin, p- coumaric acid & THZ/Metformin, Arecoline &Vanillic acid with the THZ/ Metformin have been illustrated. This review has also discussed the synergy and mechanism of phytochemical with the OHDs to combat hyperglycemia and other risk associated with it. A comprehensive review was conducted to pile up the information about polyphenols & synthetic drug combinations used for the treatment of diabetes mellitus, which has been carried out in-vitro or in-vivo and may contribute to identifying novel strategies in the treatment of T2D condition. This review shows the importance of the responsible bioactive agents present in medicinal plants in the drive to demonstrate their antidiabetic effects.

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Ellagic acid in Emblica officinalis exerts anti-diabetic activity through the action on β-cells of pancreas

Abstract: Ellagic acid in EO exerts anti-diabetic activity through the action on β-cells of pancreas that stimulates insulin secretion and decreases glucose intolerance.

Cited by 130 publications

References 32 publications

LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

Chemical composition and pharmacological activities of Pisum sativum

Hybrid drug combination: A new treatment strategy for type 2 diabetes- A review

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