Ellipsometry and polarized light

Azzam, R. M. A.; Bashara, N. M.; Burns, D. Thorburn

doi:10.1016/s0003-2670(00)82849-4

Cited by 1,528 publications

(1,793 citation statements)

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“…Reflection ellipsometry of the chitosan films was performed using a custom-built self-nulling, single wavelength (λ ) 632.8 nm) ellipsometer 13 and a commercial Multiskop instrument from Optrel GbR. In the ellipsometry experiments, the ellipsometric angles P and A corresponding to a null in the intensity at the detector were measured at fixed values of the angle of incidence θi ranging from 30 o to 60 o .…”

Section: Experimental Methodsmentioning

confidence: 99%

Effect of Changes in Relative Humidity and Temperature on Ultrathin Chitosan Films

Murray

Dutcher

2006

Biomacromolecules

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We have prepared uniform films of chitosan with thicknesses 20 nm < h < 200 nm by spincoating solutions of chitosan dissolved in dilute acetic acid onto silicon substrates while controlling the spin speed and the relative humidity inside the spincoater. After neutralizing the films, they readily absorbed water in the presence of high humidity. Heating of the films to elevated temperatures caused a large irreversible decrease in the film thickness, a small increase in the index of refraction, and a reduction in water absorption, as measured using ellipsometry. Comparisons of infrared absorption measurements of chitosan films collected before and after heating indicate an increase in the degree of acetylation with heating. Collectively, these observations are consistent with the release of bound water and a chemical change similar to acetylation at elevated temperatures.

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Section: Experimental Methodsmentioning

confidence: 99%

Effect of Changes in Relative Humidity and Temperature on Ultrathin Chitosan Films

Murray

Dutcher

2006

Biomacromolecules

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“…The change of elliptical polarization upon reflexion is related to the thickness and refractive index of the adlayer (Azzam & Bashara, 1977). Let the state of polarization of a beam be characterized by s/, the ratio of the amplitudes of the p and s polarizations:…”

Section: Ellipsometrymentioning

confidence: 99%

Experimental methods for investigating protein adsorption kinetics at surfaces

Ramsden¹

1994

Quart. Rev. Biophys.

177

131

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The adsorption of proteins at the solid-liquid interface is a process of fundamental importance in nature. Extensive reviews (MacRitchie, 1978; Andrade & Hlady, 1986; Norde, 1986) testify to the strong interest which has been shown in the problem during the past few decades. Norde & Lyklema (1978) have rightly pointed out that protein adsorption is scientifically intriguing; the phenomenology is complicated and includes many presently apparently irreconcilable observations.

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“…The polarization-dependent tensor describing SHG has up to 18 unique tensor elements defining its orientation and polarization-dependent response, compared with just three for linear optics (i.e. the three principal refractive indices; Hubbard, 1995;Azzam & Bashara, 1988). Consequently, a significantly greater amount of information is available from detailed nonlinear optical polarization measurements and analysis relative to analogous linear effects such as birefringence.…”

Section: Introductionmentioning

confidence: 99%

Polarization-resolved second-harmonic generation microscopy as a method to visualize protein-crystal domains

DeWalt

Begue

Ronau

et al. 2012

Acta Crystallogr D Biol Cryst

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Polarization-resolved second-harmonic generation (PR-SHG) microscopy is described and applied to identify the presence of multiple crystallographic domains within protein-crystal conglomerates, which was confirmed by synchrotron X-ray diffraction. Principal component analysis (PCA) of PR-SHG images resulted in principal component 2 (PC2) images with areas of contrasting negative and positive values for conglomerated crystals and PC2 images exhibiting uniformly positive or uniformly negative values for single crystals. Qualitative assessment of PC2 images allowed the identification of domains of different internal ordering within protein-crystal samples as well as differentiation between multi-domain conglomerated crystals and single crystals. PR-SHG assessments of crystalline domains were in good agreement with spatially resolved synchrotron X-ray diffraction measurements. These results have implications for improving the productive throughput of protein structure determination through early identification of multi-domain crystals.

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Ellipsometry and polarized light

Cited by 1,528 publications

References 0 publications

Effect of Changes in Relative Humidity and Temperature on Ultrathin Chitosan Films

Effect of Changes in Relative Humidity and Temperature on Ultrathin Chitosan Films

Experimental methods for investigating protein adsorption kinetics at surfaces

Polarization-resolved second-harmonic generation microscopy as a method to visualize protein-crystal domains

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