Elucidating the role of Cyclooxygenase‐2 in the pathogenesis of oral lichen planus – an immunohistochemical study with supportive histochemical analysis

Singh, Pratyush; Grover, Jasleen; Byatnal, Aditi Amit; Guddattu, Vasudeva; Radhakrishnan, Raghu; Solomon, Monica Charlotte

doi:10.1111/jop.12521

Cited by 5 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An important question concerns which of the inflammatory mediators found in OLP is responsible for the increased COX-2 expression. Relatively little work has been undertaken in this respect, but Singh et al, [37] reported a strong association of COX-2 immunoreactivity with both basement membrane integrity and the mast cell count in OLP. Mast cells are an important source of TNF-α, and so we were interested to determine whether the findings in OLP could be mimicked by treating cells with TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Relative Deficiency of Anti-Inflammatory N-Acylethanolamines Compared to Prostaglandins in Oral Lichen Planus

et al. 2020

View full text Add to dashboard Cite

Oral lichen planus (OLP) is a chronic inflammatory oromucosal disease. The N-acylethanolamines (NAEs), are a family of endogenous biologically active lipid mediators, with palmitoylethanolamide (PEA) being of particular interest here due to its anti-inflammatory and analgesic properties. In this study using oral mucosa biopsies from OLP patients and healthy controls, we investigated whether NAE synthesis was mobilized in response to the inflammation associated with OLP. PTGS2 levels, coding for cyclooxygenase-2 (COX-2), were increased approximately 4-fold in OLP compared to controls and a significant increase in the ratio of PTGS2 to NAPEPLD, the latter coding for a key enzyme in NAE synthesis, was seen. This was matched by an increased ratio of COX-2-derived prostaglandins to PEA in a second patient cohort. We conclude that there is an imbalance between prostaglandins and PEA in OLP, opening up the possibility that PEA might be a useful treatment for this disorder.

show abstract

Section: Discussionmentioning

confidence: 99%

Relative Deficiency of Anti-Inflammatory N-Acylethanolamines Compared to Prostaglandins in Oral Lichen Planus

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The slides were evaluated under bright field research microscope using computerized image software (NIS, Nikon) to provide morphometric analysis of COX‐2 expression. All tissue sections were observed under light microscope at ×100 magnification to determine the areas that presented with highest number of COX‐2 positive cells and then their quantification was done at ×400 magnification in five representative areas by predefined criteria (Singh et al, 2017). The degree of staining was scored as score 0, no positive cells; score 1, < 5% positive cells; score 2, 5%–30% positive cells and score 3 for>30% positive cells.…”

Section: Methodsmentioning

confidence: 99%

“…Along with the cells various enzymes like proteases, matrix metalloproteinases and cyclooxygenase (COX)‐2 are also involved in the pathophysiology of periapical lesions. COX‐2, induced in response to growth factors, hormones and cytokines is a plasma membrane bound enzyme that plays a key role in the growth and spread of inflammatory reactions and cystic processes (Álvares et al, 2018; Singh et al, 2017). It is thought to be involved in the pathogenesis of periapical lesions, nevertheless additional information is warranted to elucidate its role (AkbarzadehBaghban et al, 2017; Álvares et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical evaluation of cyclooxygenase‐2 and mast cell density in periapical lesions

et al. 2023

View full text Add to dashboard Cite

Aim: Periapical granuloma (PG) and cyst (PC) are formed as a protective response consequent to pulpal infection leaching through the apical foramen and lateral canals. Various inflammatory mediators like mast cells and cyclooxygenase (COX)-2 are involved in this intricate process. This pilot study aimed to evaluate and compare the immunoexpression of tryptase and COX-2 in periapical granuloma and periapical cyst, and also correlate them with intensity of inflammatory infiltrate and thickness of cystic epithelial lining.Methodology: An observational and cross-sectional study was conducted on paraffin-embedded tissue sections of 50 PGs and 50 PCs submitted for morphological and immunohistochemical analysis using anti-tryptase and anti-COX-2 antibodies. The mean number of mast cells (total, granulated and degranulated), mean COX-2 expression and inflammatory score was calculated. The data obtained were analysed using Mann Whitney U, Student's T, Chi-square and Spearman correlation test (p < .05). Results:The inflammatory score, total mast cells and COX-2 expression were similar in PGs and PCs (p = .352, .339 and .352) however, the degranulated mast cells were highly significant in PC while granulated mast cells were highly significant in PG respectively (p < .001 in both). Although a non-significant correlation existed between COX-2 and total mast cells in both groups but, total mast cells were significantly correlated with epithelial thickness in PC (p = .029).Conclusions: Mast cells and cyclooxygenase-2 proved to be independent inflammatory markers in periapical lesions. Further studies should be planned on mast cell and COX-2 inhibitors as treatment modalities of periapical lesions.

show abstract

Black pepper and its bioactive constituent piperine: promising therapeutic strategies for oral lichen planus

2018

View full text Add to dashboard Cite

Elucidating the role of Cyclooxygenase‐2 in the pathogenesis of oral lichen planus – an immunohistochemical study with supportive histochemical analysis

Abstract: Interaction between upregulation of COX-2, mast cell and basement membrane sets a vicious cycle which relates to the chronic nature of the disease. Inhibitors of COX-2 may reduce the inflammatory process preceding the immune dysregulation in OLP.

Cited by 5 publications

References 29 publications

Relative Deficiency of Anti-Inflammatory N-Acylethanolamines Compared to Prostaglandins in Oral Lichen Planus

Relative Deficiency of Anti-Inflammatory N-Acylethanolamines Compared to Prostaglandins in Oral Lichen Planus

Immunohistochemical evaluation of cyclooxygenase‐2 and mast cell density in periapical lesions

Black pepper and its bioactive constituent piperine: promising therapeutic strategies for oral lichen planus

Contact Info

Product

Resources

About