Elucidating the role of jasmonic acid in oil accumulation, fatty acid composition and gene expression in Chlorella vulgaris (Trebouxiophyceae) during early stationary growth phase

Jusoh, Malinna; Loh, Saw Hong; Chuah, Tse Seng; Aziz, Azimon Abdul; San, Thye

doi:10.1016/j.algal.2015.02.020

Cited by 71 publications

(22 citation statements)

References 45 publications

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“…At the end of fermentation, the lipid productivity reached 1.16 g/L/h with the combined addition strategy, which was about threefold higher than that of the control group. These results were in contrast with several reports on the application of other plant growth regulators, such as jasmonic acid and indole-3-acetic acid, which induced lipid accumulation only during the early growth phase (Jusoh et al 2015 ).…”

Section: Resultscontrasting

confidence: 99%

Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA

Sun

Ren

et al. 2018

AMB Expr

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Enhancing lipid productivity and reducing oxidative damage is essential for lipid overproduction in microalgae. In this study, addition of 20 mg/L fulvic acid (FA) resulted a 34.4% increase of lipid yield in Schizochytrium sp. Furthermore, the cooperative effect of FA and EDTA on cell growth and lipid production was investigated. The combined addition of 20 mg/L FA and 1.0 g/L EDTA yielded a maximal cell dry weight of 130.7 g/L and lipid productivity of 1.16 g/L/h, representing 36.4% and threefold increase over the non-supplemented group, respectively. Moreover, compared with the non-supplemented group, the combined addition strategy exhibited overall lower levels of reactive oxygen species and malondialdehyde, which accompanied with 66.7% and 81.9% higher superoxide dismutase and catalase activity, respectively. Furthermore, a 24.1–37.1% increase of malic enzyme and 19.4–25.2% decrease of phosphoenolpyruvate carboxylase activity was observed during the entire fermentation stage (0–108 h). Results suggested that the combined addition strategy not only enhanced lipid accumulation, but also prevented the lipid peroxidation.

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Section: Resultscontrasting

confidence: 99%

Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA

Sun

Ren

et al. 2018

AMB Expr

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“…In our study, the fatty acid profiles were shown in Table 1, and were mainly composed of C16 and C17 FAs. Such fatty acid profiles were basically the same as those reported in [35,51], where JA-treated and IAA-treated C. vulgaris cells produced a high proportion of C16-C18 fatty acids. Mu et al found that fatty acid (C16-C18) compositions were also indispensable to endow excellent cold filter plugging point for fuels produced by algal biomass [60].…”

Section: The Lipids and High Value Pufas Analysissupporting

confidence: 71%

“…Earlier study reported that auxin is involved in control of many developmental processes in plants [49]. Exogenous application of phytohormone could not only stimulate microalgae growth but also trigger intracellular lipid biosynthesis at different levels [13,35,50,51].…”

Section: The Lipids and High Value Pufas Analysismentioning

confidence: 99%

The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain

Ahmed

Lin

et al. 2017

Energies

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Abstract:In the present study, eight kinds plant growth regulators-salicylic acid (SA), 1-naphthaleneacetic acid (NAA), gibberellic acid (GA 3 ), 6-benzylaminopurine (6-BA), 2, 4-epi-brassinolide (EBR), abscisic acid (ABA), ethephon (ETH), and spermidine (SPD)-were used to investigate the impact on microalgal biomass, lipid, total soluble protein, carotenoids, and polyunsaturated fatty acids (PUFAS) production of Chlorella pyrenoidosa ZF strain. The results showed the quickest biomass enhancement was induced by 50 mg·L −1 NAA, with a 6.3-fold increase over the control; the highest protein content was increased by 0.005 mg·L −1 ETH, which produced 3.5-fold over the control; total carotenoids content was induced most effectively by 1 mg·L −1 NAA with 3.6-fold higher production than the control; the most efficient elicitor for lipid production was 5 mg·L −1 GA 3 at 1.9-fold of the control; 0.2 mg·L −1 ETH induced the abundant production of 1.82 ± 0.23% linoleic acid; 0.65 ± 0.01% linolenic acid was induced by 1 mg·L −1 NAA; 2.53 ± 0.15% arachidonic acid and 0.44 ± 0.05% docosahexaenoic acid were induced by 5 mg·L −1 GA 3 . Transcriptional expression levels of seven lipid-related genes, including ACP, BC, FAD, FATA, KAS, MCTK, and SAD, were studied by real-time RT-q-PCR. 5 mg·L −1 GA 3 was the most effective regulator for transcriptional expressions of these seven genes, producing 23-fold ACP, 31-fold BC, 25-fold FAD, 6-fold KAS, 12-fold MCTK compared with the controls, respectively.

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“…The major lipids in Chlorella are similar to those of other algae, but the nutritional requirements and fatty acids produced vary between species (Duong et al, 2015). Lipids and oils in Chlorella are deposited mainly in the form of triacylglycerols (TAGs), which originate from de novo fatty acid synthesis (Jusoh et al, 2015). Green algae usually use the prokaryotic pathway to synthesize chloroplast lipids.…”

Section: Introductionmentioning

confidence: 97%

Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

Aziz¹,

Osman²,

San³

et al. 2016

bta

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Phosphate is involved in fatty acid biosynthesis in microalgae cells. The influence of phosphate concentrations on polyunsaturated fatty acids' profile, elongation, and desaturation of fatty acids chains during their biosynthesis still remains unclear. Protein ketoacyl-ACP synthase-I (KAS-1 ) is required for the addition of malonyl-CoA for the elongation of butyryl-ACP from a 4-to 14-carbon chain. The omega-6 (ω-6 FAD ) and omega-3 desaturases (ω-3 FAD ) are involved in the insertion of double bonds into the pre-formed fatty acid chains. This study reports the effect of phosphate concentrations on the growth, fatty acid content, and level of KAS-1, ω-6 FAD, and ω-3 FAD transcripts in Chlorella KS-MA2 strain at the stationary growth phase. Phosphate was tested at concentrations 9, 18, 36, and 72 μM and the fatty acid content was analyzed using gas chromatography. Gene expression levels were measured using quantitative real-time-PCR. Results showed that cell growth, amount of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFAs) did not significantly (P > 0.05) differ among tested treatments. The highest concentration of α-linolenic acid and the expression level of ω-3 FAD were recorded with 72 μM of phosphate in culture medium. The KAS-1 and ω-6 FAD transcripts levels decreased when the concentration of phosphate was below 36 μM. Phosphate in F/2 medium was sufficient to enhance the biosynthesis of major fatty acids in Chlorella sp.

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Elucidating the role of jasmonic acid in oil accumulation, fatty acid composition and gene expression in Chlorella vulgaris (Trebouxiophyceae) during early stationary growth phase

Cited by 71 publications

References 45 publications

Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA

Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA

The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain

Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

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