Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA

Sun, Xiao‐Man; Ren, Lu‐Jing; Ji, Xiao‐Jun; Huang, He

doi:10.1186/s13568-018-0681-5

Cited by 32 publications

(14 citation statements)

References 45 publications

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“…The addition of fulvic acid and EDTA during the cultivation of Schizochytrium sp. HX-308 showed a 44.7% and 81.9% increase in the activities of superoxide dismutase and catalase [19]. Along similar lines, our study revealed that the addition of exogenous antioxidants leads to the upregulation of catalase in strain PKU#Mn4, which is one of the key antioxidant enzymes that mediates intracellular ROS scavenging.…”

Section: Exogenous Antioxidants Trigger Differential Expression Of Lipid Biosynthetic Genessupporting

confidence: 79%

“…Considerable efforts were made in the past few decades to optimize the culture conditions for the efficient production of fatty acids by oleaginous microalgae [18]. Particularly, 2 of 13 strategies based on ROS regulation were recently proposed to avoid the undesirable cell death and peroxidation of fatty acids induced by excessive ROS [18,19]. For example, the manipulation of the enzymatic antioxidant system through the overexpression of superoxide dismutase has shown up to a 38.5% decline in ROS level along with 32.9% higher PUFA production in the engineered strain of Schizochytrium sp.…”

Section: Introductionmentioning

confidence: 99%

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Exogenous Antioxidants Improve the Accumulation of Saturated and Polyunsaturated Fatty Acids in Schizochytrium sp. PKU#Mn4

et al. 2021

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Species of Schizochytrium are well known for their remarkable ability to produce lipids intracellularly. However, during their lipid accumulation, reactive oxygen species (ROS) are generated inevitably as byproducts, which if in excess results in lipid peroxidation. To alleviate such ROS-induced damage, seven different natural antioxidants (ascorbic acid, α-tocopherol, tea extract, melatonin, mannitol, sesamol, and butylated hydroxytoluene) were evaluated for their effects on the lipid accumulation in Schizochytrium sp. PKU#Mn4 using a fractional factorial design. Among the tested antioxidants, mannitol showed the best increment (44.98%) in total fatty acids concentration. However, the interaction effects of mannitol (1 g/L) and ascorbic acid (1 g/L) resulted in 2.26 ± 0.27 g/L and 1.45 ± 0.04 g/L of saturated and polyunsaturated fatty acids (SFA and PUFA), respectively, in batch fermentation. These concentrations were further increased to 7.68 ± 0.37 g/L (SFA) and 5.86 ± 0.03 g/L (PUFA) through fed-batch fermentation. Notably, the interaction effects yielded 103.7% and 49.6% increment in SFA and PUFA concentrations in batch fermentation. The possible mechanisms underlining those increments were an increased maximum growth rate of strain PKU#Mn4, alleviated ROS level, and the differential expression of lipid biosynthetic genes andupregulated catalase gene. This study provides an applicable strategy for improving the accumulation of SFA and PUFA in thraustochytrids by exogenous antioxidants and the underlying mechanisms.

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Section: Exogenous Antioxidants Trigger Differential Expression Of Lipid Biosynthetic Genessupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Exogenous Antioxidants Improve the Accumulation of Saturated and Polyunsaturated Fatty Acids in Schizochytrium sp. PKU#Mn4

et al. 2021

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“…In order to identify and evaluate the potential effect of phytohormone supplementation on the growth and the overall DHA production of SW1, a screening experiment with eight phytohormones from six different classes, including auxin, cytokinin, gibberellin, signal transducer, melatonin and amine, was conducted (Table 1). The selection of phytohormones and their concentration ranges were based on our preliminary screening as well as the previous studies from miscellaneous organisms [16][17][18][19][20][26][27][28]. Each of the phytohormones was used in eight different concentrations, as shown in Table 1.…”

Section: Screening Of Phytohormones For Improved Growth and Dha Production Of Sw1mentioning

confidence: 99%

“…have successfully alleviated the oxidative stress and increased the DHA content in the microalgae [13][14][15]. However, the primary effect of the antioxidants was only to reduce the oxidative damage, rather than inducing the growth, lipid and DHA biosynthesis [16].…”

Section: Introductionmentioning

confidence: 99%

“…Phytohormones have also been proposed not only to reduce the oxidative damage in cells but also to increase the growth and lipid production by controlling the internal biochemical pathways [19]. For example, the addition of 20 mg/L fulvic acid (FA) and 1 mg/L ethylenediaminetetraacetic acid (EDTA) resulted in a 36.4% and 34.4% increase in biomass and lipid yield in Schizochytrium spp., which exhibited a lower ROS and MDA level accompanied with higher superoxide dismutase (SOD) and catalase (CAT) activity in comparison to the control [16]. Furthermore, Yu et al [17] revealed that the addition of auxins indole acetic acid (IAA) and naphthylacetic acid (NAA) increased the lipid productivity up to threefold under nitrogen-depleted conditions as compared to standard BG-11 medium, by modulating the oxidative damage caused by ROS during the cultivation.…”

Section: Introductionmentioning

confidence: 99%

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Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

et al. 2020

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In the present study, the impact of eight phytohormones from six different classes on the growth, lipid and docosahexaenoic acid (DHA) biosynthetic capacity of Aurantiochytrium sp. SW1 (SW1) was evaluated. Kinetin (KIN), jasmonic acid (JA) and gibberellic acid (GA) significantly enhanced the growth and DHA production of SW1 by 16%–28% and 66%–84% in comparison to the control, respectively. The synergistic effect of these three phytohormones, evaluated by the response surface methodology (RSM), showed that a combination of 3.6 mg/L GA, 2.0 mg/L KIN and 20.0 mg/L JA further increased the growth and DHA production of SW1 by 16% to 28% and 22% to 36%, respectively, in comparison to the individual supplementation. The synergistic effect of these phytohormones was also shown to be time-dependent, where feeding at 24 h of cultivation led to 15%, 26% and 35% further increments in the biomass, lipid and DHA production in comparison to that of 0 h, respectively. The determination of stress markers, antioxidant enzymes and key enzymes involved in fatty acid biosynthesis aided to elucidate the potential mechanism underlying the improvement of growth and DHA production by SW1 at various times of feeding. Supplementation with the phytohormones at 24 h exhibited the maximum impact on reducing the level of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as augmented the antioxidants (superoxide dismutase and catalase) and key metabolic enzymes involved in lipogenesis (malic, glucose-6-phosphate dehydrogenase and ATP-citrate lyase) in comparison to the control and other time points. This study signifies the potential application of phytohormones for improving the growth, lipid and DHA production in Aurantiochytrium spp.

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Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

Zhang

et al. 2023

Biotechnology Journal

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In order to find a more effective way to obtain docosahexaenoic acid (DHA) rich lipid from Schizochytrium sp., a widespread propionate wastewater (PW) is used. PW is a common industrial and domestic wastewater, and transforming it into valuable products is a potential treatment method. Schizochytrium sp. is a rapidly growing oleaginous organism, which has been used commercially for DHA production. Herein, PW is completely used for DHA production by Schizochytrium sp. by genetic engineering and fermentation optimization, which can alleviate the increasingly tense demand for water resources and environmental pollution caused by industrial wastewater. Firstly, the methylmalonyl‐CoA mutase (MCM) was overexpressed in Schizochytrium sp. to enhance the metabolism of propionate, then the engineered strain of overexpressed MCM (OMCM) can effectively use propionate. Then, the effects of PW with different concentration of propionate were investigated, and results showed that OMCM can completely replace clean water with PW containing 5 g L−1 propionate. Furthermore, in the fed‐batch fermentation, the OMCM obtained the highest biomass of 113.4 g L−1 and lipid yield of 64.4 g L−1 in PW condition, which is 26.8% and 51.7% higher than that of wild type (WT) in PW condition. Moreover, to verify why overexpression of MCM can promote DHA and lipid accumulation, the comparative metabolomics, ATP production level, the antioxidant system, and the transcription of key genes were investigated. Results showed that ATP induced by PW condition could drive the synthesis of DHA, and remarkably improve the antioxidant capacity of cells by enhancing the carotenoids production. Therefore, PW can be used as an effective and economical substrate and water source for Schizochytrium sp. to accumulate biomass and DHA.

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Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA

Cited by 32 publications

References 45 publications

Exogenous Antioxidants Improve the Accumulation of Saturated and Polyunsaturated Fatty Acids in Schizochytrium sp. PKU#Mn4

Exogenous Antioxidants Improve the Accumulation of Saturated and Polyunsaturated Fatty Acids in Schizochytrium sp. PKU#Mn4

Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

Enhancing the biomass and docosahexaenoic acid‐rich lipid accumulation of Schizochytrium sp. in propionate wastewater

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