Elution of in vivo bound antiprostatic epithelial antibodies following multiple cryotherapy of carcinoma of prostate

Ablin, Richard J.; Soanes, Ward A.; Gonder, Maurice J.

doi:10.1016/0090-4295(73)90463-9

Cited by 39 publications

(20 citation statements)

References 14 publications

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“…The freezing-stimulated change in the immune response is another proposed mechanism of cryosurgery. Although few animal models and clinical case studies describe a ''cryoimmunologic response'' and even spontaneous regression of the metastases following cryoablation (5), the majority of studies prove no change in the immune status (4). Moreover, others suggest suppressed immunity, increased tumor growth, and metastasis after cryoablation of primary tumors (6).…”

mentioning

confidence: 99%

Combined Dendritic Cell Cryotherapy of Tumor Induces Systemic Antimetastatic Immunity

Machlenkin

Goldberger

Tirosh

et al. 2005

Clinical Cancer Research

106

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Purpose: Cryotherapy of localized prostate, renal, and hepatic primary tumors and metastases is considered a minimally invasive treatment demonstrating a low complication rate in comparison with conventional surgery. The main drawback of cryotherapy is that it has no systemic effect on distant metastases. We investigated whether intratumoral injections of dendritic cells following cryotherapy of local tumors (cryoimmunotherapy) provides an improved approach to cancer treatment, combining local tumor destruction and systemic anticancer immunity. Experimental Designs: The 3LL murine Lewis lung carcinoma clone D122 and the ovalbumintransfected B16 melanoma clone MO5 served as models for spontaneous metastasis. The antimetastatic effect of cryoimmunotherapy was assessed in the lung carcinoma model by monitoring mouse survival, lung weight, and induction of tumor-specific CTLs. The mechanism of cryoimmunotherapy was elucidated in the melanoma model using adoptive transfer of T cell receptor transgenic OT-I CTLs into the tumor-bearing mice, and analysis of Th1/Th2 responses by intracellular cytokine staining in CD4 and CD8 cells. Results: Cryoimmunotherapy caused robust and tumor-specific CTL responses, increased Th1 responses, significantly prolonged survival and dramatically reduced lung metastasis. Although intratumor administration of dendritic cells alone increased the proliferation rate of CD8 cells, only cryoimmunotherapy resulted in the generation of effector memory cells. Furthermore, cryoimmunotherapy protected mice that had survived primary MO5 tumors from rechallenge with parental tumors. Conclusions: These results present cryoimmunotherapy as a novel approach for systemic treatment of cancer. We envisage that cryotherapy of tumors combined with subsequent in situ immunotherapy by autologous unmodified immature dendritic cells can be applied in practice.Minimally invasive therapies are an alternative approach to surgical intervention in the treatment of malignant diseases. Cryoablation, i.e., tissue destruction by repeated deep freezing and thawing, is under the larger category of thermal therapy and, during the past decade, it has become an acceptable clinical tool for the management of dermatologic tumors, hepatocellular carcinoma, renal and prostate tumors, and hepatic colorectal metastases (1, 2). Compared with surgical excision, the main advantages are the potential for less invasiveness resulting in reduced mortality and morbidity, and the ability to perform ablative procedures on outpatients, which decreases the treatment cost. In the case of hepatic colorectal metastases, the use of cryosurgery improves the percentages of resectability (2). A comparative study on domestic pigs showed that the cryoablation of renal parenchyma is beneficial over other necrosis-inducing ablations such as microwave thermoablation, radiofrequency energy, and chemoablation by ethanol, hypertonic saline, and acetic acid gels, in terms of reproducibility, consistency in size and shape, and the ability to monitor b...

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mentioning

confidence: 99%

Combined Dendritic Cell Cryotherapy of Tumor Induces Systemic Antimetastatic Immunity

Machlenkin

Goldberger

Tirosh

et al. 2005

Clinical Cancer Research

106

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“…Antibodies observed up to 30 days post-cryo, notably reactive with the caudal lobe, may have become bound in situ to autologous antigen and thus been removed from the circulation. This also remains as a plausible explanation for the observed diminution in circulating antibodies and has been observed in a patient with prostatic cancer following cryotherapy [5]. Despite these findings, the possible effect of humoral factors on histologic alterations in the prostate cannot be excluded.…”

Section: Discussionmentioning

confidence: 54%

“…The cross-reactivity of human and monkey prostatic tissue-specific antigens [2], and the identification of antibodies to human prostatic fluid [3] and in patients with prostatic cancer [4,5] when reacted with monkey prostatic tissue have been observed. The monkey thus appeared to be a suitable potential experimental model for the induction of prostatic cancer and the subsequent evaluation of the treatment of prostatic malignancy by cryotherapy.…”

Section: Abstract: Cellular Response -Cryosurgery -Histopathology -Hmentioning

confidence: 99%

Immunologic and morphologic effects of cryosurgery of the monkey (Macaque) prostate

Reddy

Ablin

1979

Res. Exp. Med.

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A reduction in size of the Macaque prostate following single and multiple in situ freezings was accompanied by histologic alterations, which occurred predominately in the caudal lobe and were characterized by what appeared to be specific periacinar foci of lymphocytic infiltrates. These lymphocytes were observed to infiltrate onto acinar epithelial cells, resulting in their subsequent separation from the basal lamina and epithelial destruction. Other histologic alterations were in consonance with studies of the prostate following cryosurgery by others. Circulating antibodies specific for prostate were present in only one of the seven animals at the time at which these histologic observations were made. These observations suggest the possible development of a cellular immunologic response following cryosurgery of the prostate. The relevancy of these observations to those obtained following cryoprostatectomy in man and other species is considered. Pending confirmation, they may be of potential significance in providing an explanation of reported cases of eradication of human prostatic carcinomas following cryotherapy.

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“…)-specific or tumour-associated antigens are liberated into the circulation in response to freezing insult. It has been suggested (9,12) that as a result of the liberation of prostatic epithelial antigens, and presumably the occurrence of antigen excess, that any pre-existing and/or newly developed (that is, in response to freezing) antibodies combine in vivo with their common antigen; that is, they become cell bound and are removed from the circulation.…”

Section: Discussionmentioning

confidence: 99%

Serum Proteins in Prostatic Cancer

Rj¹,

Wa²,

Mj³

1977

Urol Int

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Levels of the three major serum immunoglobulins (G, A, M) were quantitated prior to and up to 2 weeks following each of two independent freezing treatments of the primary prostatic tumour in 4 patients undergoing remission or healing of their metastatic lesions. Cyclical alterations in the level of IgG accompanied by moderate alterations in the level of IgA were noted post-operatively. Most striking was an almost twofold increase in the level of IgM in 3 of the 4 patients evaluated. While possibly a non-specific association, observations of increased levels of IgG and IgM following cryotherapy may represent an active immunologic response by the host to prostatic tissue (tumour?)-specific or tumour-associated antigen(s) in the form of ‘blocking’ factor(s) or cytotoxic antibody.

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Elution of in vivo bound antiprostatic epithelial antibodies following multiple cryotherapy of carcinoma of prostate

Cited by 39 publications

References 14 publications

Combined Dendritic Cell Cryotherapy of Tumor Induces Systemic Antimetastatic Immunity

Combined Dendritic Cell Cryotherapy of Tumor Induces Systemic Antimetastatic Immunity

Immunologic and morphologic effects of cryosurgery of the monkey (Macaque) prostate

Serum Proteins in Prostatic Cancer

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