Embedded passivated-electrode insulator-based dielectrophoresis (EπDEP)

Shake, Tyler; Zellner, Phillip; Sahari, Ali; Breazeal, Maria V. Riquelme; Behkam, Bahareh; Pruden, Amy; Agah, Masoud

doi:10.1007/s00216-013-7435-7

Cited by 10 publications

(12 citation statements)

References 24 publications

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“…Another creative approach called embedded passivated‐electrode iDEP (EπDEP) was proposed by the Agah research group . This hybrid dielectrophoretic mode combines iDEP and eDEP as it employs devices that contain both electrodes and insulating structures.…”

Section: Advances On Devices For Trapping Idepmentioning

confidence: 99%

On the recent developments of insulator‐based dielectrophoresis: A review

2018

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Insulator-based dielectrophoresis (iDEP), also known as electrodeless DEP, has become a well-known dielectrophoretic technique, no longer viewed as a new methodology. Significant advances on iDEP have been reported during the last 15 years. This review article aims to summarize some of the most important findings on iDEP organized by the type of dielectrophoretic mode: streaming and trapping iDEP. The former is primarily used for particle sorting, while the latter has great capability for particle enrichment. The characteristics of a wide array of devices are discussed for each type of dielectrophoretic mode in order to present an overview of the distinct designs and applications developed with iDEP. A short section on Joule heating effects and electrothermal flow is also included to highlight some of the challenges in the utilization of iDEP systems. The significant progress on iDEP illustrates its potential for a large number of applications, ranging from bioanalysis to clinical and biomedical assessments. The present article discusses the work on iDEP by numerous research groups around the world, with the aim of proving the reader with an overview of the state-of-the-art in iDEP microfluidic systems.

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Section: Advances On Devices For Trapping Idepmentioning

confidence: 99%

On the recent developments of insulator‐based dielectrophoresis: A review

2018

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“…[4][5][6][7] Electrode based DEP (eDEP) technique is normally used to generate non-uniform electric fields in the channel. 8,9 Micro-patterned electrodes in the channel generate highly localized electric fields and trapping is observed to be concentrated around the electrodes. Insulator based DEP (iDEP), on the other hand, uses insulating structures rather than embedded electrodes to a) D. Nakidde, P. Zellner, and M. M. Alemi contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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In this study, a 3D passivated-electrode, insulator-based dielectrophoresis microchip (3D pDEP) is presented. This technology combines the benefits of electrodebased DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The 3D pDEP chips were fabricated by making 3D structures in silicon using reactive ion etching. The reusable electrodes are deposited on second glass substrate and then aligned to the microfluidic channel to capacitively couple the electric signal through a 100 lm glass slide. The 3D insulating structures generate high electric field gradients, which ultimately increases the DEP force. To demonstrate the capabilities of 3D pDEP, Staphylococcus aureus was trapped from water samples under varied electrical environments. Trapping efficiencies of 100% were obtained at flow rates as high as 350 ll/h and 70% at flow rates as high as 750 ll/h. Additionally, for live bacteria samples, 100% trapping was demonstrated over a wide frequency range from 50 to 400 kHz with an amplitude applied signal of 200 Vpp. 20% trapping of bacteria was observed at applied voltages as low as 50 Vpp. We demonstrate selective trapping of live and dead bacteria at frequencies ranging from 30 to 60 kHz at 400 Vpp with over 90% of the live bacteria trapped while most of the dead bacteria escape. V C 2015 AIP Publishing LLC.

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“…Heterogeneous cell populations’ solation into more distinct homogenous subpopulations will enable further molecular, chemical, or biophysical characterization of the cellular activities. Such detailed analysis can be further utilized for the monitoring of diseases in patients or detecting harmful pathogen activities in the environment [5–8]. Specifically, potentially high‐risk bioparticles, such as nonviable cells, cancerous cells, disease‐infected cells, and bacterial cells could be separated from surrounding (nontarget) particles and evaluated.…”

Section: Introductionmentioning

confidence: 99%

Passivated‐electrode insulator‐based dielectrophoretic separation of heterogeneous cell mixtures

Kikkeri

Kerr

Bertke

et al. 2020

J of Separation Science

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Rapid and accurate purification of various heterogeneous mixtures is a critical step for a multitude of molecular, chemical, and biological applications. Dielectrophoresis has shown to be a promising technique for particle separation due to its exploitation of the intrinsic electrical properties, simple fabrication, and low cost. Here, we present a geometrically novel dielectrophoretic channel design which utilizes an array of localized electric fields to separate a variety of unique particle mixtures into distinct populations. This label-free device incorporates multiple winding rows with several nonuniform structures on to sidewalls to produce high electric field gradients, enabling high locally generated dielectrophoretic forces. A balance between dielectrophoretic forces and Stokes' drag is used to effectively isolate each particle population. Mixtures of polystyrene beads (500 nm and 2 μm), breast cancer cells spiked in whole blood, and for the first time, neuron and satellite glial cells were used to study the separation capabilities of the design. We found that our device was able to rapidly separate unique particle populations with over 90% separation yields for each investigated mixture. The unique architecture of the device uses passivated-electrode insulator-based dielectrophoresis in an innovative microfluidic device to separate a variety of heterogeneous mixture without particle saturation in the channel. K E Y W O R D Sbreast cancer, circulating tumor cells, dielectrophoresis, neuron cells, satellite glial cells 1576

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Embedded passivated-electrode insulator-based dielectrophoresis (EπDEP)

Cited by 10 publications

References 24 publications

On the recent developments of insulator‐based dielectrophoresis: A review

On the recent developments of insulator‐based dielectrophoresis: A review

Three dimensional passivated-electrode insulator-based dielectrophoresis

Passivated‐electrode insulator‐based dielectrophoretic separation of heterogeneous cell mixtures

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