Embossing of micropatterned ceramics and their cellular response

Abstract

The aim of this work is to investigate the use of microtopographies in providing physical cues to modulate the cellular response of human mesenchymal stem cells on ceramics. Two microgrooved patterns (100 μm/50 μm, 10 μm/10 μm groove/pitch) were transcribed reversely onto alumina green ceramic tapes via an embossing technique followed by sintering. Characterization of the micropatterned alumina surfaces and their cellular response was carried out. Spread and polygonal cell morphologies were observed on the wider groove (50 μm/100 μm) surface. Cells seeded onto the narrow groove (10 μm/10 μm) surface aligned themselves alongside the grooves, resulting in more elongated cell morphology. More osteoid matrix nodules shown by osteopontin and osteocalcin biomarkers were detected on the larger grooved surfaces after cell culture of 21 days, indicating a greater level of osteogenicity. This study has shown that micropatterned wider groove (50 μm) topographies are more suitable surfaces for improving osseointegration of ceramic implants.

Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimentalin vitrostudy

“…Nadeem et al. investigated the effects of micropatterned surfaces on cell responses. These authors created different sized microgrooves (100 μm/50 μm, and 10 μm/10 μm groove/pitch) on ceramic disks, which were then seeded with human mesenchymal stem cells.…”

“…It was found that cultured human osteoblasts preferred surfaces with relatively high microroughness, which generated increased cell activity. Nadeem et al 2013 investigated the effects of micropatterned surfaces on cell responses. These authors created different sized microgrooves (100 lm/50 lm, and 10 lm/10 lm groove/pitch) on ceramic disks, which were then seeded with human mesenchymal stem cells.…”

Scanning electron microscopical observation of an osteoblast/osteoclast co-culture on micropatterned orthopaedic ceramics

“…18 In vitro stem cell co-cultures allow observation of the complex interactions between BMSC and BMHC and provide the most accurate model for assessing biomaterials and their effect on both bone formation and normal bone turnover. 19 Several authors report successful co-cultures of osteoblast cell lines or primary osteoblasts combined with peripheral blood mononuclear cells (PBMC) or isolated monocytes, usually of murine or human origin, cultivated with the addition of macrophage colony-stimulating factor (M-CSF). 20–22 Previously, the authors have reported co-cultures based on BMSC.…”

Sensing the Difference: The Influence of Anisotropic Cues on Cell Behavior

“…Also, while structural orthopedic ceramics, such alumina/zirconia, tend to be highly bio-inert, metals such as titanium and its alloys are highly adhesive to cells due to their reactive oxide layers and both these low and high adhesion environments can mask topographical effects. However, recent advancements in pre-sintering embossing of green ceramics have illustrated that groove patterns in alumina with different widths can be used to influence fibrous tissue (narrow width, 20 µm pitch) and hard tissue (150 µm pitch with 100 µm groove/50 µm ridge) growth from human osteoprogenitor cells (Nadeem et al, 2013), similarly to results observed on polymers (Biggs et al, 2008).…”