Embryo and Ovary Culture of Important Legume Horse Gram, Macrotyloma uniflorum (Lam.) Verdc

Abstract

Background and Objective: Macrotyloma uniflorum is nutritious and a good source of bioactive substances with high biological processes. The high frequency and efficient in vitro regeneration protocol have developed first time by utilizing embryo and ovary explants in Horsegram, Macrotyloma uniflorum. Materials and Methods: This research was carried out from horse gram paiyur-2 variety seeds derived embryo and ovary explants used by MS medium supplemented with cytokinins and auxins. Results: The phytohormone 0.4 mg LG 1 zeatin induced a maximum of 12 and 9 shoots from an embryo and ovary explants, respectively. The maximum frequency of rooting with about 9 roots was procured from the shoots cultured from embryonic explants using 0.2 mg LG 1 NAA and 7 roots were propagated from shoots derived from ovary explants. Overall studies observed that the embryonic explants show a high percent frequency of shoot and root induction when compared to ovary culture and further plantlets rose using embryonic explants with shoot growth period than the ovary explants. Conclusion: In vitro legume regeneration utilizing embryo and ovary is efficient, dependable and has fewer problems. Embryo explants were used to induce high-frequency plant regeneration, indicating that the ovaries are useful in genetic transformation.