Embryo Culture in Explanted Oviducts in Mice and Cattle

Hosoi, Yoshihiko; Minami, Naojiro; Iritani, Akira

doi:10.1159/000184654

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…Moreover, mouse ampullas can even provide suitable conditions for further development of hamster 2-cell embryos 36 , and overcome the 2-cell block 37 . The species-unspecific nature of this type of culture was repeatedly proved by producing rat [38][39][40] , porcine 41 and bovine blastocysts 42,43 . Contrarily, we were not able to find a proper strategy to support the activation of the embryo genome in the conventional IVEC.…”

Section: Discussionmentioning

confidence: 99%

In vitro production of naked mole-rats’ blastocysts from non-breeding females using in vitro maturation and intracytoplasmic sperm injection

Simone,

Čižmár,

Holtze

et al. 2023

Sci Rep

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The African naked mole-rat (Heterocephalus glaber) is an attractive model for cancer and aging research due to its peculiar biological traits, such as unusual long life span and resistance to cancer. The establishment of induced pluripotent stem cells (iPSCs) would be a useful tool for in vitro studies but, in this species, the reprogramming of somatic cells is problematic because of their stable epigenome. Therefore, an alternative approach is the derivation of embryonic stem cells from in vitro-produced embryos. In this study, immature oocytes, opportunistically retrieved from sexually inactive females, underwent first in vitro maturation (IVM) and then in vitro fertilization via piezo-intracytoplasmic sperm injection (ICSI). Injected oocytes were then cultivated with two different approaches: (i) in an in vitro culture and (ii) in an isolated mouse oviduct organ culture system. The second approach led to the development of blastocysts, which were fixed and stained for further analysis.

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Section: Discussionmentioning

confidence: 99%

In vitro production of naked mole-rats’ blastocysts from non-breeding females using in vitro maturation and intracytoplasmic sperm injection

Simone,

Čižmár,

Holtze

et al. 2023

Sci Rep

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“…(Krisher et al 1989). De même, les cocultures hétérospéci-fiques d'embryons bovins dans des explants d'oviductes de souris (Hosoi et al 1995), pendant 46 à 48 heures, permettent d'obtenir des taux de développement jusqu'au stade blastocyste beaucoup plus importants (42,9 %) que ceux obtenus avec une coculture de cellules de la granulosa (28,3 %). Des explants d'oviductes de mouton ont également été utilisés avec succès pour cultiver des embryons bovins produits in vitro (Rizos et al 2002a).…”

Section: Explants D'oviductesunclassified

Microenvironnement tubaire: rôle dans la maturation des ovocytes canins in vivo et in vitro

Fontaine¹,

Reynaud²,

Thoumire³

et al. 2009

bavf

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Chez la plupart des mammifères, les ovocytes sont bloqués en métaphase II au moment de l'ovulation et cette inhibition de la méiose est ensuite levée par la fécondation. Chez les chiennes et les autres femelles de canidés, les ovocytes sont libérés au stade de prophase I, et il faut encore attendre 48 à 72 heures pour qu'ils atteignent le stade de métaphase II et deviennent fécondables. Cette particularité constitue aujourd'hui un frein au développement des biotechnologies de la reproduction chez les canidés. En effet, dans les essais de maturation in vitro d'ovocytes canins, seuls 10 à 30 % des ovocytes atteignent le stade de métaphase au bout de 72 heures de culture. Chez la chienne, la maturation nucléaire se produisant dans l'oviducte, des substituts de l'oviducte (milieux de culture comme le Synthetic Oviductal Fluid, explants d'oviductes, cultures sur tapis de cellules tubaires) ont été utilisés pour les cultures d'ovocytes in vitro, afin d'en améliorer le rendement, mais sans grand succès jusqu'à maintenant. Cet échec peut être dû au manque de données sur la composition du liquide tubaire de la chienne. L'étude de ce microenvironnement prend donc tout son intérêt, celui-ci étant probablement assez différent de celui des autres femelles, ne serait-ce que par l'existence du processus de lutéinisation préovulatoire dans cette espèce. À terme, la conception d'un milieu de maturation sur la base de la composition du liquide tubaire pourrait être une voie intéressante pour augmenter les taux de maturation in vitro. Mots

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Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture

Rizos

Pintado

Fuente

et al. 2006

Molecular Reproduction Devel

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The aim of this study was to examine the development of bovine zygotes in isolated mouse oviducts (IMO) and the quality of the blastocysts produced. In vitro produced bovine zygotes were transferred into the ampullae of the IMO and cultured in SOF or KSOM. Control embryos were cultured in droplets of the same media. Following 6 days of culture, blastocysts were processed for nuclei counts or mRNA abundance. Culture in the IMO did not affect the proportion of zygotes developing to the blastocyst stage compared to the respective control droplets (SOF: 17.7 +/- 3.2% vs. 18.8 +/- 2.7%; KSOM: 20.7 +/- 2.6% vs. 22.2 +/- 2.8%). Culture in the IMO in KSOM resulted in an increased number of inner cell mass (ICM) nuclei; however, total nuclei number or incidence of apoptosis was unaffected. Culture in the IMO in SOF resulted in an increase (P < 0.05) in abundance of transcripts in blastocysts for Oct-4 and SOX, and reduced abundance of Glut-1, Na/K, Cx43, and survivin compared to blastocysts derived from culture in SOF alone. In contrast, culture in the IMO in KSOM resulted in increased abundance of transcripts for Glut-1, Cx43, Oct-4, and survivin and reduced expression of Na/K and SOX compared to KSOM alone. Transcripts for G6PDH, IFN-tau, and E-Cad were unaffected. These data confirm that the IMO is capable of supporting development of bovine embryos. Depending on the basal medium used, the pattern of transcript abundance in embryos derived from the IMO is similar to that of in vivo derived embryos.

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Embryo Culture in Explanted Oviducts in Mice and Cattle

Cited by 6 publications

References 26 publications

In vitro production of naked mole-rats’ blastocysts from non-breeding females using in vitro maturation and intracytoplasmic sperm injection

In vitro production of naked mole-rats’ blastocysts from non-breeding females using in vitro maturation and intracytoplasmic sperm injection

Microenvironnement tubaire: rôle dans la maturation des ovocytes canins in vivo et in vitro

Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture

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