Embryo-Derived Platelet-Activating Factor Is Essential for Establishment of Pregnancy in the Mouse

Spinks, N. R.; O’Neill, Chris

doi:10.1016/s0140-6736(87)91947-7

Cited by 50 publications

(12 citation statements)

References 20 publications

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“…The protocol used by these authors, however, differed from the present investigation both in the frequency of the administration of the inhibitors and the period over which they were given, as well as in the nature of the inhibitors. In particular, Spinks & O'Neill (1987 …”

Section: )mentioning

confidence: 99%

Failure of platelet-activating factor (PAF-acether) to induce decidualization in mice and failure of antagonists of PAF to inhibit implantation

Milligan

Finn²

1990

Reproduction

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Summary. The possible role of platelet-activating factor (PAF) in the uterine responses associated with implantation was investigated. Attempts to trigger a decidual cell response in the uteri of hormonally sensitized, ovariectomized mice by instilling PAF\x=req-\ acether (1\p=n-\1000ng) intraluminally were unsuccessful. The effect of PAF antagonists on implantation was investigated in females ovariectomized on Day 3 of pregnancy and treated with progesterone. Implantation was induced in these females by injection of 10 ng oestradiol-17\g=b\ on Day 8. Hourly intraperitoneal injections of three PAF antagonists (WEB 2086, CV 3988 and BN 52021 at doses of 1\ m=. \ 2\ p=n-\ 1\ m=. \ 4 mg/kg) given over a 24-h period starting 1 h before the injection of oestradiol-17\g=b\ had no significant effect on the occurrence of implantation sites. Intraluminal injection of WEB 2086 (15 \g=m\g) or BN 52021 (5 pg) either 3 h before or 6 h after the nidatory oestradiol also had no significant inhibitory effect on implantation. given once daily over the first 4 days of pregnancy at a dose of 40 \g=m\g/30g body weight had no inhibitory effect on the establishment of pregnancy. These results are not consistent with a critical role for PAF in implantation in mice.

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Section: )mentioning

confidence: 99%

Failure of platelet-activating factor (PAF-acether) to induce decidualization in mice and failure of antagonists of PAF to inhibit implantation

Milligan

Finn²

1990

Reproduction

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“…Embryonic PAF in the rabbit and mouse increases during the preimplantation phase, with maximum levels at the expanded blastocyst stage (15,22). Embryo-derived PAF is essential for implantation and the establishment of pregnancy (23,24). Human preimplantation embryos synthesize and secrete PAF (21) and PAF is considered a biomarker indicating the likelihood of pregnancy (25).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Preimplantation Embryo Development (Ped) Gene on Embryonic Platelet-Activating Factor (PAF) Levels

Purnell

Warner

Kort

et al. 2006

J Assist Reprod Genet

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“…Partial characterization of this PAF-like activity was accomplished, including bioassay in splenectomized mice, use of inhibitors of various pathways of platelet activation and structural analysis using lipase digestion; PAF concentrations in zygotes were not measured. None the less, combined with the inhibition of implantation in mice and rats by PAF antagonists (Acker et al, 1987;Spinks & O'Neill, 1987), these data suggest a significant role for PAF in the events of early pregnancy.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, PAF is one of the most potent vasoactive mediators reported to date. In addition, a substance thought to be PAF is produced by 8 16-cell mouse embryos in culture (O'Neill, 1985a, b), and administration of a PAF antagonist inhibits implantation in the mouse (Spinks & O'Neill, 1987) and in the rat (Acker et al, 1987 Blastocysts were routinely collected from uterine flushings or the exposed endometrium of the uterus, pooled and, following lipid extraction/fractionation, assayed for PAF activity (see below). In selected experiments, blastocyst production of PAF in culture was examined.…”

Section: Introductionmentioning

confidence: 99%

Platelet-activating factor in the rabbit uterus during early pregnancy

Angle

Jones

McManus³

et al. 1988

Reproduction

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Summary. Platelet-activating factor (PAF) concentrations were low in the non-pregnant, oestrous uterus (mean \m=+-\s.e.m.: 2\m=.\2\m=+-\1\m=.\2 pmol/g, n = 3). However, uterine PAF increased dramatically during pregnancy to a maximum of 37\m=.\8\ m=+-\ 4\m=.\90pmol/g (n = 7) on Day 5. By Day 7, PAF concentrations in the uteri of pregnant rabbits had returned to levels similar to those found at oestrus. In contrast, uterine PAF in pseudopregnant rabbits peaked at 30\m=.\6\ m=+-\2\m=.\8pmol/g (n = 8) on Day 4, declined to 20\m=.\5\ m=+-\ 2\ m=. \ 4pmol/g (n = 8) on Day 5 and then remained at that concentration through Day 7. Uterine PAF co-migrated with synthetic PAF 1-0-hexadecyl-2-acetyl-sn-glycero-phosphocholine) in both thin-layer and normal-phase high-performance liquid chromatography. PAF activity in the uterus during pregnancy and pseudopregnancy was found almost exclusively in the endometrium; little or no PAF was found in myometrium, uterine flushings or blastocysts. While no PAF was detected in blastocysts on Days 5 and 6 of pregnancy, the presence of the embryo appears to modulate biosynthesis and/or degradation of PAF by the uterus, since PAF decreased significantly in uterine tissue apposed to the implanting embryo (but not in similar areas between such attachment sites). Increased concentrations of PAF in the preimplantation rabbit uterus followed by a dramatic decrease on the day of blastocyst attachment suggest that this potent inflammatory autacoid may play a vital role in implantation.

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Embryo-Derived Platelet-Activating Factor Is Essential for Establishment of Pregnancy in the Mouse

Cited by 50 publications

References 20 publications

Failure of platelet-activating factor (PAF-acether) to induce decidualization in mice and failure of antagonists of PAF to inhibit implantation

Failure of platelet-activating factor (PAF-acether) to induce decidualization in mice and failure of antagonists of PAF to inhibit implantation

Influence of the Preimplantation Embryo Development (Ped) Gene on Embryonic Platelet-Activating Factor (PAF) Levels

Platelet-activating factor in the rabbit uterus during early pregnancy

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