Influence of the Preimplantation Embryo Development (Ped) Gene on Embryonic Platelet-Activating Factor (PAF) Levels

Purnell, Elissa T.; Warner, Carol M.; Kort, Hilton I.; Mitchell-Leef, Dorothy; Elsner, Carlene W.; Shapiro, Daniel B.; Massey, J.H.; Roudebush, William E.

doi:10.1007/s10815-006-9039-z

Cited by 12 publications

(5 citation statements)

References 33 publications

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“…There is precedence for the presence vs. the absence of the genes encoding the Ped gene phenotype affecting the expression of another molecule. Absence of Qa-2 expression has been shown to result in higher expression of platelet activating factor (PAF), a protein known to be beneficial to reproductive success [34]. From the findings with PAF and the results reported in the present paper we can conclude that the presence or absence of the Qa-2 encoding genes can affect both protein and miRNA expression levels in preimplantation embryos.…”

Section: Discussionsupporting

confidence: 66%

MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice

Byrne

Warner

2008

J Assist Reprod Genet

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Section: Discussionsupporting

confidence: 66%

MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice

Byrne

Warner

2008

J Assist Reprod Genet

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“…These genomic regions harbor strong candidate genes implicated in cellular functions related to placental development and fetal growth, such as PAFAH2, CLIC4, and WNT4 (BTA2, 126.70–128.26 Mb), and CHAC1, CHST14 , and DLL4 (BTA10, 33.62–35.56 Mb). The PAFAH2 gene has been implicated in various stages of reproduction, including implantation, fetal development, and parturition 26 . The PAFAH2 gene stimulates the formation of IP3 and DAG and increases intracellular calcium 27 .…”

Section: Resultsmentioning

confidence: 99%

Genes and pathways associated with pregnancy loss in dairy cattle

Sigdel

Bisinotto

Peñagaricano

2021

Sci Rep

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Pregnancy loss directly impairs reproductive performance in dairy cattle. Here, we investigated genetic factors associated with pregnancy loss following detection of a viable embryo around 42 days of gestation. The objectives of this study were to perform whole-genome scans and subsequent gene-set analyses for identifying candidate genes, functional gene-sets and gene signaling pathways implicated in pregnancy loss in US Holstein cows. Data consisted of about 58,000 pregnancy/abortion records distributed over nulliparous, primiparous, and multiparous cows. Threshold models were used to assess the binary response of pregnancy loss. Whole‐genome scans identified at least seven genomic regions on BTA2, BTA10, BTA14, BTA16, BTA21, BTA24 and BTA29 associated with pregnancy loss in heifers and lactating cows. These regions harbor several candidate genes that are directly implicated in pregnancy maintenance and fetal growth, such as CHST14, IGF1R, IGF2, PSEN2, SLC2A5 and WNT4. Moreover, the enrichment analysis revealed at least seven significantly enriched processes, containing genes associated with pregnancy loss, including calcium signaling, cell–cell attachment, cellular proliferation, fetal development, immunity, membrane permeability, and steroid metabolism. Additionally, the pathway analysis revealed a number of significant gene signaling pathways that regulate placental development and fetal growth, including Wnt, Hedgehog, Notch, MAPK, Hippo, mTOR and TGFβ pathways. Overall, our findings contribute to a better understanding of the genetic and biological basis of pregnancy loss in dairy cattle and points out novel strategies for improving pregnancy maintenance via marker‐assisted breeding.

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“…The uniformly poor development of B6 embryos (whether cultured individually or communally) and the benefits of exogenous tropic ligands, indicate that embryos of this strain may have a profound deficiency of production of autocrine trophic ligands in vitro. It has been shown that the release of the autocrine embryotrophic ligand (Paf) is under genetic control [ 22 ] and this might also apply to other autocrine trophic ligands. The well characterised high level of sensitivity of B6 strain embryos to culture in vitro [ 19 ] has been shown to be associated with the loss of P53 regulation by trophic ligands in vitro [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…The inbred C57BL/6 J (B6) strain is particularly sensitive to culture in simple defined media, compared to the robust growth response of a hybrid strain [ 19 ]. Of interest, the release of putative trophic ligands may also be associated to the genetic background in mice [ 20 - 22 ].…”

Section: Introductionmentioning

confidence: 99%

Systematic analysis of the factors that adversely affect the rate of cell accumulation in mouse embryos during their culture in vitro

Jin

O’Neill

2014

Reprod Biol Endocrinol

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BackgroundRetarded embryo growth is a pervasive effect of culture in vitro.MethodsA systematic analysis of the interactions between media design, embryo culture density, oxygen tension, amino acids, trophic ligands and the genetic background of the mouse on embryo growth rates in vitro was performed.ResultsGrowth retardation of mouse zygotes was greater in 20% O2 than 5%, a sequential media design was superior to static simple media designs, but the supplementation of simple media with mixed amino acids mitigated this difference. There was a beneficial effect of communal culture in small volumes, and supplementation with a trophic ligand (Paf) further enhanced growth rates. For hybrid strain zygotes (B6CBF1) communal culture in KSOM media supplemented with amino acids, albumin and Paf under 5% O2 resulted in complete rescue of their rate of accumulation of cells and blastocyst formation. Inbred strain (C57BL6/J) zygotes, however, still showed some retardation of development under these conditions. The additional supplementation of media with another trophic ligand (IGF1) showed a further additive beneficial effect on development of inbred strain embryos but they still showed a growth deficit of ~ 23% cell number. The results show that optimising the interactions between a range of culture conditions and media design can rescue hybrid strain embryos from a retarded rate of cell proliferation caused by culture in vitro, but this was incomplete for the B6 strain.ConclusionsThe results indicate that the growth requirement of embryos in vitro varies depending upon their genetic background and provide models for the further genetic analysis of embryo growth.

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Influence of the Preimplantation Embryo Development (Ped) Gene on Embryonic Platelet-Activating Factor (PAF) Levels

Cited by 12 publications

References 33 publications

MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice

MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice

Genes and pathways associated with pregnancy loss in dairy cattle

Systematic analysis of the factors that adversely affect the rate of cell accumulation in mouse embryos during their culture in vitro

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