MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice

Byrne, Michael; Warner, Carol M.

doi:10.1007/s10815-008-9211-8

Cited by 24 publications

(17 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These miRNAs were not found in previous studies, probably because of the use of different detection methods for miRNAs (6)(7)(8)(9), different mouse strains (17), and embryos with or without culture (6,8).…”

Section: Discussionmentioning

confidence: 63%

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Liu

Pang

Chiu

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

386

335

View full text Add to dashboard Cite

In mammals, the sperm deliver mRNA of unknown function into the oocytes during fertilization. The role of sperm microRNAs (miRNAs) in preimplantation development is unknown. miRNA profiling identified six miRNAs expressed in the sperm and the zygotes but not in the oocytes or preimplantation embryos. Sperm contained both the precursor and the mature form of one of these miRNAs, miR-34c. The absence of an increased level of miR-34c in zygotes derived from α-amanitin-treated oocytes and in parthenogenetic oocytes supported a sperm origin of zygotic miR-34c. Injection of miR-34c inhibitor into zygotes inhibited DNA synthesis and significantly suppressed first cleavage division. A 3′ UTR luciferase assay and Western blotting demonstrated that miR-34c regulates B-cell leukemia/lymphoma 2 (Bcl-2) expression in the zygotes. Coinjection of anti-Bcl-2 antibody in zygotes partially reversed but injection of Bcl-2 protein mimicked the effect of miR-34c inhibition. Oocyte activation is essential for the miR-34c action in zygotes, as demonstrated by a decrease in 3′ UTR luciferase reporter activity and Bcl-2 expression after injection of precursor miR-34c into parthenogenetic oocytes. Our findings provide evidence that sperm-borne miR-34c is important for the first cell division via modulation of Bcl-2 expression.

show abstract

Section: Discussionmentioning

confidence: 63%

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Liu

Pang

Chiu

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

386

335

View full text Add to dashboard Cite

show abstract

“…miR-125b was used as an endogenous control to normalize the target miRNA because this miRNA is expressed consistently in preimplantation mouse embryos [26]. …”

Section: Methodsmentioning

confidence: 99%

Molecular cloning and expression of bovine nucleoplasmin 2 (NPM2): a maternal effect gene regulated by miR-181a

Lingenfelter

Tripurani

Tejomurtula

et al. 2011

Reprod Biol Endocrinol

View full text Add to dashboard Cite

BackgroundNucleoplasmin 2 (NPM2) is an oocyte-specific nuclear protein essential for nuclear and nucleolar organization and early embryonic development. The aims of this study were to clone the bovine NPM2 gene, determine its temporal expression during oocyte development and early embryogenesis, and evaluate the potential role of miRNA-181a in regulation of its expression.MethodsA 329 bp cDNA fragment was amplified from bovine fetal ovary using primers designed based on the conserved regions of the human and mouse NPM2 cDNA sequences. RACE experiments were performed to obtain the 5' and 3' ends of the bovine NPM2 cDNA. Real time PCR and Western blot analysis were used to examine the expression of bovine NPM2 in oocytes and early embryos. Co-expression of bovine NPM2 and miRNA-181a in Hela cells was performed to determine if expression of bovine NPM2 is regulated by miRNA-181a.ResultsThe bovine NPM2 cDNA is 851 bp in length encoding a protein of 200 amino acids. The protein contains the conserved bipartite nuclear localization sequence and shows 53% and 62% identity with mouse and human NPM2, respectively. Expression of bovine NPM2 mRNA is restricted to ovaries. NPM2 mRNA is abundant in GV and MII stage oocytes, decreases in early cleavage stage embryos, and barely detectable in morula and blastocyst stage embryos. Similarly, expression of NPM2 protein is high in oocytes and early embryos but extremely low in blastocysts. The abundance of NPM2 mRNA is significantly lower in oocytes isolated from persistent versus growing dominant follicles (P < 0.05). A miR-181a binding site in the 3'UTR of the NPM2 transcript was identified. Transfection experiments showed that bovine NPM2 protein expression is reduced in Hela cells expressing miR-181a compared to control cells without miR-181a, indicating that translation of NPM2 is repressed by miR-181a.ConclusionsOur data suggest that expression of bovine NPM2 is temporally regulated during early embryogenesis and miR-181a may play a role in its regulation.

show abstract

“…In particular, miR-125b, which is known to regulate cell proliferation [9,10] and differentiation [11][12][13], has been shown to be required for the development of lower organisms, such as Drosophila [14] and zebrafish [15]. The expression of miR-125b is also found in mouse preimplantation embryos although its exact role has not been eluciated [16]. Several proteins, including p53, have been reported to be the targets of miR-125b and mediate its action in various physiological and pathological events, including neuronal differentiation [12,17,18], immune response [19,20] and cancer [10,[21][22][23].…”

Section: Hcomentioning

confidence: 99%

“…Taken together, these results suggest that CFTR is involved in mediating the effect of extracellular HCO 3 − on early-embryo development. 3 − influx in mouse embryo development Since miR-125b, which is known to be involved in cellular differentiation and the development of lower organisms [15,35], is expressed in mouse preimplantation embryo [16], we attempted to test whether HCO 3 − may act as an environmental cue regulating miR-125b levels and hence embryo development. Using real-time PCR, we first examined the expression profile of miR-125b at different stages of mouse preimplantation embryo development and the results showed a relatively high level of miR-125b at two-cell and four-cell stages, but a significantly lower level in morula and blastocyst ( Figure 2A), indicating that miR-125b may be required for early cleavage of embryos, but no longer induced at higher differentiation stage.…”

Section: − -Dependent Preimplantation Embryo Developmentmentioning

confidence: 99%

CFTR mediates bicarbonate-dependent activation of miR-125b in preimplantation embryo development

et al. 2012

View full text Add to dashboard Cite

show abstract

MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice

Abstract: Our results show that the absence of the Ped gene profoundly affects the level of a miRNA (miR-125a) known to regulate early development. The implication is that miR-125a is likely involved in the regulation of timing of early development in mice.

Cited by 24 publications

References 49 publications

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Molecular cloning and expression of bovine nucleoplasmin 2 (NPM2): a maternal effect gene regulated by miR-181a

CFTR mediates bicarbonate-dependent activation of miR-125b in preimplantation embryo development

Contact Info

Product

Resources

About