Embryo development after vitrification of immature and in vitro-matured equine oocytes

Angel, Daniel; Canesin, H.S.; Brom-de-Luna, J.G.; Morado, Sergio Adrián; Dalvit, Gabriel Carlos; Gómez, Diana Sanzon; Posada, Natalia; Pascottini, Osvaldo Bogado; Urrego, Rodrigo; Hinrichs, Katrin; Velez, I. C.

doi:10.1016/j.cryobiol.2020.01.014

Cited by 11 publications

(13 citation statements)

References 14 publications

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“…Although these live births represent a significant accomplishment, the efficiency of the procedure remains extremely low. Several contributing factors have been associated to the lack of progress in the equine species, including the length of exposure to cryoprotectant combinations [6,7,8,9], nuclear maturation status [8,10,11,12], presence or absence of cumulus investments [13], cytoskeletal damage and microtubule depolymerization [6,7], meiotic spindle and chromosomal aberrations [14]. Other factors affecting equine oocyte vitrification were recently reviewed by De Coster et al [15].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin

Clérico

Taminelli

Veronesi³

et al. 2021

Theriogenology

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Section: Introductionmentioning

confidence: 99%

Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin

Clérico

Taminelli

Veronesi³

et al. 2021

Theriogenology

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“…In the first step, the oocytes are incubated for a relatively long time (10–15 min) in equilibration solution, and the second step consists of exposure to the vitrification solution for 30–90 s [ 55 , 56 , 57 ]. However, for horse oocytes, despite good maturation and cleavage rates and some blastocyst development, the relatively long exposure to CPAs has been associated with CPA toxicity, affecting subsequent embryo development [ 11 , 13 , 58 , 59 ]. Tharasanit et al, 2006 [ 28 ] demonstrated that a short protocol did not exhibit toxicity in horse oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Only a few foals have been born resulting from mature or immature oocytes cryopreserved by vitrification [ 9 , 10 ]. The vitrification of immature and in vitro matured equine oocytes compromises embryo development after ICSI [ 11 , 12 , 13 , 14 ]. Hence, in order to exploit the application potential of equine oocyte vitrification, further optimization of the vitrification protocols is necessary (for review see De Coster & Angel-Velez, et al, 2020 [ 5 ]).…”

Section: Introductionmentioning

confidence: 99%

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

Angel‐Velez

Coster

Azari‐Dolatabad

et al. 2021

Animals

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Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency. Therefore, we aimed to perform a direct comparison of non-permeating and permeating cryoprotective agents (CPAs) during the vitrification and warming of equine immature oocytes. In the first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, and galactose in combination with ethylene glycol (EG) and dimethyl sulfoxide (DMSO). In the second experiment, the COCs were vitrified using three mixtures of permeating CPAs in a 50:50 volume ratio (ethylene glycol-dimethyl sulfoxide (ED), propylene glycol-ethylene glycol (PE), and propylene glycol-dimethyl sulfoxide (PD)) with galactose and warmed in different galactose concentrations (0.3 or 0.5 mol/L). Overall, all the treatments supported blastocyst formation, but the developmental rates were lower for all the vitrified groups in the first (4.3 to 7.6%) and the second (3.5 to 9.4%) experiment compared to the control (26.5 and 34.2%, respectively; p < 0.01). In the first experiment, the maturation was not affected by vitrification. The sucrose exhibited lower cleavage than the control (p = 0.02). Although the galactose tended to have lower maturation than trehalose (p = 0.060) and control (p = 0.069), the highest numerical cleavage and blastocyst rates were obtained with this CPA. In the second experiment, the maturation, cleavage, and blastocyst rates were similar between the treatments. Compared to the control, only the ED reached similar maturation (p = 0.02) and PE similar cleavage (p = 0.1). The galactose concentration during warming did not affect the maturation, cleavage, or blastocyst rates (p > 0.1), but the PE-0.3 exhibited the highest blastocyst rate (15.1%) among the treatments, being the only one comparable to the control (34.2%). As such, PE–galactose provides a valuable option for equine immature oocyte vitrification and should be considered for the future optimization of the protocol.

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“…As OPU is well established in horses and oocyte shipment is in general necessary, the cryopreservation of equine oocytes before ICSI is a promising technique which requires more studies. Indeed, the first foal born after oocyte vitrification and ICSI has been reported recently [ 195 ].…”

Section: Comparison Of Assisted Reproduction Techniques In Horses and Humansmentioning

confidence: 99%

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

Benammar

Derisoud

Vialard

et al. 2021

Animals

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Although there are large differences between horses and humans for reproductive anatomy, follicular dynamics, mono-ovulation, and embryo development kinetics until the blastocyst stage are similar. In contrast to humans, however, horses are seasonal animals and do not have a menstrual cycle. Moreover, horse implantation takes place 30 days later than in humans. In terms of artificial reproduction techniques (ART), oocytes are generally matured in vitro in horses because ovarian stimulation remains inefficient. This allows the collection of oocytes without hormonal treatments. In humans, in vivo matured oocytes are collected after ovarian stimulation. Subsequently, only intra-cytoplasmic sperm injection (ICSI) is performed in horses to produce embryos, whereas both in vitro fertilization and ICSI are applied in humans. Embryos are transferred only as blastocysts in horses. In contrast, four cells to blastocyst stage embryos are transferred in humans. Embryo and oocyte cryopreservation has been mastered in humans, but not completely in horses. Finally, both species share infertility concerns due to ageing and obesity. Thus, reciprocal knowledge could be gained through the comparative study of ART and infertility treatments both in woman and mare, even though the horse could not be used as a single model for human ART.

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Embryo development after vitrification of immature and in vitro-matured equine oocytes

Cited by 11 publications

References 14 publications

Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin

Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

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