Embryo score is a better predictor of pregnancy than the number of transferred embryos or female age

Terriou, Philippe; Sapin, Christophe; Giorgetti, C.; Hans, E.; Spach, Jean-Louis; Roulier, R.

doi:10.1016/s0015-0282(00)01741-6

Cited by 118 publications

(78 citation statements)

References 12 publications

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“…Also, recombinant class I MHC molecules can be engineered to include GPI tails so that they can be used for painting [15]. Given that painting with Qa-2 increases the rate of embryo cleavage [8], and that rapid cleavage is a trait of the healthiest human embryos observed during assisted reproduction [2], painting with GPI tail-engineered HLA-G may have therapeutic potential. (A) Membrane bound and soluble isoforms of human leukocyte antigen G (HLA-G) and Qa-2.…”

Section: Discussionmentioning

confidence: 99%

“…Embryonic expression of Qa-2 provides a range of advantageous phenotypes, including faster preimplantation cleavage rate, better survival to term, and higher birth and weaning weights compared with embryos lacking the gene [1]. During clinical assisted reproductive procedures, faster cleaving embryos are associated with more successful pregnancy outcomes [2]. This phenomenon suggests the existence of a Ped-like gene in humans.…”

Section: Introductionmentioning

confidence: 99%

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Evidence that HLA-G is the functional homolog of mouse Qa-2, the ped gene product

et al. 2003

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Qa-2, a murine class Ib major histocompatibility complex (MHC) molecule, is a possible functional homolog of human leukocyte antigen G (HLA-G). Both molecules have been implicated in immunoregulation and embryonic development and both occur in membrane-bound and soluble isoforms that arise by alternative splicing. Soluble splice variants have been implicated in the reproductive functions of HLA-G. While soluble variants of Qa-2 have been previously detected in T lymphocytes, we now demonstrate the presence of mRNA for one of the two known soluble forms of Qa-2 in eight-cell embryos and in blastocysts. Qa-2 is glycosylphosphatidy-linositol (GPI) linked in the outer leaflet of the cell membrane and is found in lipid raft microdomains where other raftassociated proteins transduce signals into the cell. In contrast, HLA-G has a truncated six amino acid cytoplasmic tail. By fluorescence co-localization in JEG-3 cells, using fluorescent cholera toxin β subunit (a lipid raft marker) and anti-HLA-G antibody, we have demonstrated that membrane-bound HLA-G also localizes to lipid rafts, consistent with functional homology between the two molecules. Finally, our experiments in which we have purified Qa-2 and transferred it via a process known as protein painting to Qa-2 negative cells represent a model for potential therapy involving HLA-G.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence that HLA-G is the functional homolog of mouse Qa-2, the ped gene product

et al. 2003

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“…As other groups (6,7,48,49), we have proposed a modified cumulative embryo score (35,50) from PN evaluation until day transfer. Following such criterion the embryo selected to be transferred (top quality embryo) must feature good PN morphology (S0), two cells at 26 h after ICSI, and also be classified as "good quality" on day 3.…”

Section: Discussionmentioning

confidence: 99%

“…Several non-invasive strategies can be used to improve end-results: pronuclei disposition, nucleolar organization (1-3), assessment of cell number, the morphological appearance at the time of transfer, and identification of normal-cleaving embryos with only mononucleate blastomeres without fragmentation (4)(5)(6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

The impact of pronuclear orientation to select chromosomally normal embryos

Borges

Rossi

Farah

et al. 2005

J Assist Reprod Genet

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Purpose : To find an effective method to select embryos from ICSI with better chromosomal status when preimplantation-genetic-diagnosis (PGD) is not applied. Methods : Several morpholological evaluations were done in same embryos. Embryos from ICSI were classified on day 1 according to pronuclear-nucleoli arrangement. On day 3, classification was done according to number, fragmentation, size and shape of cells. In some patients, embryos exhibiting good quality on day 3 (at least six regular blastomeres with cell fragmentation lower than 20%) were also submitted to PGD, irrespective to pronuclearnucleoli morphology. Results : Forty-two per cent of normally fertilized embryos showed pronuclear-nucleoli-goodmorphology; from those, 86% were also classified as good quality on day 3. Good-quality embryos submitted to PGD have shown lower chromosomal abnormality rates when also classified as pronuclear-nucleoli-good-morphology. Conclusions : Pronuclear-nucleoli morphology seems to be correlated with PGD results. This criterion may prove useful for pre-select embryos with normal chromosomal package when PGD is not applied.

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“…SET produces an unacceptably low pregnancy rate particularly in older patients and in those with a poor embryo quality (De Neubourg and Gerris 2006). The woman's age and embryo quality are the variables with most influence on the implantation rate (Steer et al 1992;Shulman et al 1993;Giorgetti et al 1995;Van Royen et al 1999;Hardarson et al 2001;Terriou et al 2001;Hunault et al 2002;Holte et al 2007). The first variable is unchangeable, but when there is a sufficient number of embryos available we can select the embryos with the greatest implantation potential according to morphological criteria for transfer (Van Royen et al 1999;Ebner et al 2001;Van Royen et al 2001;Scott et al 2002;Ebner et al 2003;Scott et al 2003;Van Royen et al 2003;Rienzi et al 2005;Holte et al 2007;Scott et al 2007).…”

Section: Introductionmentioning

confidence: 99%

A minimally invasive methodology based on morphometric parameters for day 2 embryo quality assessment

Molina

Lázaro-Ibáñez

Pertusa

et al. 2014

Reproductive BioMedicine Online

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The risk to maternal-foetal health due to multiple pregnancies can be decreased by reducing the number of transferred embryos. Therefore, new tools for selecting embryos with the highest implantation potential should be developed. The aim of this study is to evaluate the predictive implantation ability of morphological and morphometric variables by analyzing images of embryos. This is a retrospective study of 135 embryo photographs from 112 IVF-ICSI cycles performed between January and March 2011. The embryos were photographed immediately before transfer using "Cronus 3" software and their images were analyzed using the public program ImageJ.Significant effects and higher discriminant power to predict implantation were observed for the morphometric embryo variables in comparison with morphological ones. The features for successfully implanted embryos were: 4-cells on day 2 of development; all blastomeres with circular shape (roundness factor greater than 0.9) ), an average ZP thickness of 13 microns and an average of 17695.1 microns 2 for the embryo area. The size of the embryo, which is described by its area and the average roundness factor for each cell, provides two objective variables to consider when predicting implantation. This method would improve the current "embryo classification systems".

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Embryo score is a better predictor of pregnancy than the number of transferred embryos or female age

Cited by 118 publications

References 12 publications

Evidence that HLA-G is the functional homolog of mouse Qa-2, the ped gene product

Evidence that HLA-G is the functional homolog of mouse Qa-2, the ped gene product

The impact of pronuclear orientation to select chromosomally normal embryos

A minimally invasive methodology based on morphometric parameters for day 2 embryo quality assessment

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