Embryobildung durch isolierte Einzelzellen aus Gewebekulturen vonDaucus carota

Genetic Regulation of Embryonic Pattern Formation

“…Is this also the case in plants? The observation that plants can form complete organisms from cultured cells in a process that resembles zygotic embryogenesis argues against a strict requirement for maternal information (Backs-Hü semann and Reinert, 1970;Nomura and Komamine, 1985;Mordhorst et al, 2002). Nevertheless, several findings imply that in normal development maternal tissues do affect embryo patterning.…”

Embryogenesis: A New Start in Life.

“…In maize, for example, the cytoplasm and nucleus are shifted toward the apical end upon fertilization of the egg cell (Mbl et al, 1994). Although somatic embryos demonstrate that apical-basal polarity can be established without maternal information (Backs-Hüsemann and Reinert, 1970;Nomura and Komamine, 1985), the strict correlation between the orientation of the apical-basal a i s of the embryo and the structure of the ovule suggests that such information could play an important role in zygotic embryogenesis.…”

The Scientific Roots of Modern Plant Biotechnology

“…They cultured single carrot cells from suspension cultures on microscope slides where they could be observed and photographed repeatedly. Isolated cells divided to form a mass of embryogenic and parenchyma cells, and the embryogenic cells developed into heart-shaped and torpedoshaped embryos with recognizable cotyledons, hypocotyls, and radicles (Backs-Hü semann and Reinert, 1970).…”