Embryogenesis in Citrus tissue cultures

Spiegel-Roy, P.; Kochba, J.

doi:10.1007/3540098070_7

Cited by 20 publications

(12 citation statements)

References 96 publications

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“…The formation of compact nodular embryogenic callus and embryoids and the regeneration of plants via somatic embryogenesis reported here for avocados correspond to results found for somatic cells of the nucellus and immature ovules of various Citrus cultivars (Button et al 1974;Spiegel-Roy and Kochba 1980).…”

Section: Histologysupporting

confidence: 87%

Induction of embryogenesis in callus from immature embryos of Persea americana

Mooney¹,

Staden²

1987

Can. J. Bot.

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MOONEY, P. A., and VAN STADEN, J. 1987. Induction of embryogenesis in callus from immature embryos of Persea americana. Can. J. Bot. 65: 622-626. Both the external morphology and internal anatomy of developing somatic embryoids and plantlets of Persea americana (avocados) were studied. The embryogenic callus, which is embryoidal in origin, was made up of normal parenchymatous tissue toward the centre of a clump of cells and numerous proembryoidal cells on the periphery of the callus mass. Embryogenesis appeared to begin in single cells on the periphery of the callus and from cells on the surface of existing proembryoids. Embryo initials could be recognised by their conspicuous starch contents and by their thickened walls, which lack plasmodesmata. Cell divisions occurred in cells with thickened walls to give rise to globular proembryoids. Proembryoids developed into heart-shaped, torpedo-shaped and cotyledonary embryoids and finally into complete plantlets. MOONEY, P. A., et VAN STADEN, J. 1987. Induction of embryogenesis in callus from immature embryos of Persea americana. Can. J. Bot. 65 : 622-626. La morphologie exteme et l'anatomie interne des embryoi'des somatiques et des plantules en diffkrenciation du Persea americana (avocatier) ont Ct C Ctudiks. Le cal embryogtne qui est d'origine embryolde Ctait constituC de tissu parenchymateux normal vers le centre d'un massif de cellules et de nombreuses cellules proembryo'ides i la pCriphCrie du cal. L'embryogCntse semble avoir comrnencC dans des cellules uniques i la pCriphCrie du cal et i partir de cellules superficielles 21 la surface des proembryoides existants. Les initiums d'embryons se distinguaient par la prCsence manifeste d'amidon et par leurs parois Cpaisses et dCpoumes de plasmodesmes. Les divisions cellulaires se faisaient chez les cellules 21 parois Cpaissies pour donner naissance i des proembryoi'des globulaires. Les proembryo'ides devenaient des embryo'ides cordiformes, torpilles et cotylCdonnaires et finalement des plantules complhtes.[Traduit par la revue]

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Section: Histologysupporting

confidence: 87%

Induction of embryogenesis in callus from immature embryos of Persea americana

Mooney¹,

Staden²

1987

Can. J. Bot.

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“…Overall, fruits harvested at 120–130 DAP had higher number of fully developed seeds with functional embryos. The significant effect of fruit harvesting stage on growth and number of embryos in citrus has also been reported by many researchers (Carimi et al, ; Spiegel‐Roy & Kochba, ), and at more advanced developmental stage; however, a single embryo was noticed in the central position at the micropylar apex of the embryo sac (Carimi et al, ). Embryo age and culture media failed to exert any significant effect on days required to start of embryo germination as well as completion of germination process.…”

Section: Discussionsupporting

confidence: 58%

Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange (Citrus aurantium) × Sacaton citrumelo (C. paradisi × Poncirus trifoliata) crosses through embryo rescue

et al. 2019

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In citrus, development of new hybrids is difficult due to failure of the germination of zygotic embryos. Hence, this study was conducted to standardize embryo age and media for maximizing the germination and subsequent seedling growth in sour orange × Sacaton citrumelo crosses followed by the identification of simple sequence repeats (SSR) markers for distinguishing the hybrids. A factorial experiment in complete randomized design was conducted with two each embryo age (110–120 and 130–140 days after pollination) and culture media (G‐B5 supplemented with 1.5 mg/L GA3 plus malt extract and G‐B5 plus malt extract) for in vitro embryo culture of sour orange × Sacaton citrumelo progenies. Older embryos germinated well in both media, though highest on M1 medium (100%). In fact, M1 medium gave higher plantlet survival in both age groups. The seedling growth of older embryos had highest root length (37.80 mm) and shoot length (24.20 mm) in M1 medium at 60 days after inoculation (DAI). Three SSR markers (TAA45, CAC15 and CAC39) showed polymorphism between female and male parents and were able to identify their hybrids.

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“…Caulogenesis or adventitious shoot formation of fruit trees has only been reported in Otrus [1], Morus [16] and Prunus [10]. Somatic embryogenesis has also been reported in a few woody genera such as Carica [8], Malus [2,11], Prunus [20] and Vitis [7,13] but only in the Rutaceae has it been studied in detail [19]. In this paper, we present the first report on the techniques used to obtain plant regeneration from various explants of open-pollinated seedlings of 'Golden Delicious' apple grown in vitro and for callus derived from such leaf explants.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from apple seedling explants and callus cultures

Liu

Sink

Dennis

1983

Plant Cell Tiss Organ Cult

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Leaf, cotyledon, and hypocotyl explants were obtained from 3-week-old seedlings of open-pollinated 'Golden Delicious' (Malus domestica bork H.) grown in vitro. They were placed on modified Murashige and Skoog (MS) medium containing B5 vitamins, sucrose and agar, supplemented with 6-benzylaminopurine (BAP) and a-naphthaleneacetic acid (NAA), and maintained at 25 °C -+ 2 in the light or in the dark to assess morphogenetic responses. Leaf and cotyledon explants cultured in the dark for an initial 3 weeks, then transferred to light for 4 weeks, produced 5-to 20-fold more adventitious shoots than those cultured for 7 weeks in the light. Conversely, light did not significantly influence the number of adventitious shoots formed on hypocotyl explants. Five-minute daffy exposures of leaf explants to red light (651 rim) suppressed adventitious shoot formation by 80%; five-minute exposure to far-red light (729 nm) immediately following the red light counteracted the red suppression.Seedling explants, immature fruit halves and immature embryos were also cultured on Schenk and Hildebrandt (SH) medium containing 2, 4-dichlorophenoxyacetic acid (2, 4-D), p-chlorophenoxyacetic acid (CPA) and kinetin. Light inhibited callus formation on leaf and cotyledon explants, but not on hypocotyl explants. The derived callus was placed on MS + BAP or MS + BAP + NAA for shoot regeneration. Both shoots and roots regenerated from callus placed in the dark but not in the light; the frequency of shoot regeneration was 5% or less.Regenerated shoots were rooted on MS macronutrient salts (1/3 concentration), micronutrients, i-inositol, thiamine HC1, sucrose and agar with or without indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), or NAA under a light intensity of 5.0 W.m-2 (16 h per day). Auxin concentration strongly influenced root morphology.

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Embryogenesis in Citrus tissue cultures

Cited by 20 publications

References 96 publications

Induction of embryogenesis in callus from immature embryos of Persea americana

Induction of embryogenesis in callus from immature embryos of Persea americana

Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange (Citrus aurantium) × Sacaton citrumelo (C. paradisi × Poncirus trifoliata) crosses through embryo rescue

Plant regeneration from apple seedling explants and callus cultures

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