Embryogenic cell suspension culture induction and plantlet regeneration of<i>Rauvolfia serpentina</i>(L.) Benth.: influence of different plant growth regulator concentrations and combinations

Uikey, Devi Singh; Tripathi, Manoj Kumar; Tiwari, G.P.; Patel, R. P.; Ahuja, Ashok

doi:10.5958/0975-6892.2016.00019.8

Cited by 8 publications

(9 citation statements)

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“…The present results align with the earlier observations of Akmal et al [ 32 ], who also documented globular and heart-shaped embryoid development in a liquid medium. Liquid medium supplemented with 3.0 mg L −1 2,4-D in combination with 0.5 mg L −1 6-Benzylaminopurine(BAP) facilitated higher growth rates ( Table 3 ), this is in accordance with the reports of Mishra et al [ 25 ] for soybean, Shyam et al [ 31 ] for Indian mustard, Uikey et al [ 36 ] for Rauwolfia serpentina , and Tripathi et al [ 37 ] for sandalwood cell suspension cultures, as they recorded the highest growth rate of embryogenic tissues with the application of 2.0–3.0 mg L −1 2,4-D in combination with 0.5 mg L −1 BAP.…”

Section: Resultssupporting

confidence: 86%

In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

Shyam¹,

Tripathi²,

Tiwari³

et al. 2021

Plants

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Brassica junceais a crucial cultivated mustard species and principal oilseed crop of India and Madhya Pradesh, grown for diverse vegetables, condiments, and oilseeds. Somaclonal variation was explored as a probable source of additional variability for the manipulation of fatty acids, especially low erucic acid contents that may be valuable for this commercially important plant species. The plantlets regenerated from tissue cultures (R0), their R1 generation and respective parental lines were compared for morpho-physiological traits and fatty acid profile for the probable existence of somaclonal variations. The first putative somaclone derived from genotype CS54 contained 5.48% and 5.52% erucic acid in R0 and R1 regenerants, respectively, compared to the mother plant (41.36%). In comparison, the second somaclone acquired from PM30 exhibited a complete absence of erucic acid corresponding to its mother plant (1.07%). These putative somaclones present a source of variation for exploitation in the development of future mustard crops with low erucic acid content.

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Section: Resultssupporting

confidence: 86%

In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

Shyam¹,

Tripathi²,

Tiwari³

et al. 2021

Plants

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“…Initially, it was observed that in liquid culture, calli could not be fragmented to form suspensions of cells or small cell clumps, which could possibly be due to higher lignification. Tiwari et al [ 27 ] had similar observation for onion, Jhankare et al [ 28 ] for withania, Bariwa et al [ 29 ] for muskmelon, Uikey et al [ 30 ] for Rauvolfia serpentina , Patidar et al [ 31 ] for chitrak and Sharma et al [ 32 ] for grape cell suspension cultures in liquid medium. To obtain small cell clumps, calluses were agitated instinctively.…”

Section: Resultsmentioning

confidence: 80%

Cell Suspension Culture and In Vitro Screening for Drought Tolerance in Soybean Using Poly-Ethylene Glycol

Tripathi¹,

Tiwari²,

Tripathi

et al. 2021

Plants

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Soybean (Glycine max (L) Merrill) is used in India mostly as a substantial fund of protein and oil, which makes the crop significantly important. Somaclonal variation has been researched as a base of additional variability for drought in soybean. In the present experiment calli/cell clumps/embryoids rose from immature and mature embryonic axis and cotyledons explants were exposed to different concentrations of polyethylene glycol (PEG6000). A discontinuous method proved to be superior as it permitted the calli/embryoids/cell clumps to regain their regeneration competence. A total of 64 (12.21%) plantlets of genotype JS335 and 78 (13.13%) of genotype JS93-05 were regenerated after four consequent subcultures on the selection medium with an effective lethal concentration of 20% PEG6000, and proliferated calli/embryoids/cell clumps were further subcultured on Murashige and Skoog regeneration medium supplemented with 0.5 mgL−1 each of α-napthalene acetic acid (NAA), 6-benzyladenine (BA) and Kinetin (Kn), 20.0 gL−1 sucrose and 7.5 gL−1 agar. Putative drought-tolerant plantlets were acquired from genotype JS93-05 (38) in more numbers compared to genotype JS335 (26). Random decamer primers confirmed the presence of variability between mother plants and regenerated plants from both the genotypes. Since these plantlets recovered from tolerant calli/embryoids/cell clumps selected from the medium supplemented with PEG6000, the possibility exists that these plants may prove to be tolerant against drought stress.

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“…Contrary to the result of the present study, in most of the other reports related to shoot regeneration from calli, BA + NAA combinations were frequently used , Mallick et al 2012, Rashmi and Trivedi 2016. However, Uikey et al (2016) mentioned the usage of three PGRs interventions such as BA, TDZ, and NAA (each at a dose of 0.5 mg/l) for shoot regeneration from embryogenic callus-derived somatic embryos. In some cases, indirect (callus-mediated) shoot regeneration was obtained in the media supplemented with GA 3 along with the presence of BA (Singh et al 2009;Bahuguna et al 2011;.…”

Section: Discussionmentioning

confidence: 99%

Ameliorated reserpine production via in vitro direct and indirect regeneration system in Rauvolfia serpentina (L.) Benth. ex Kurz.

et al. 2020

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Rauvolfia serpentina (L.) Benth. ex Kurz., popularly known as Indian Snakeroot plant, belonging to Apocynaceae family, holds immense medicinal importance, owing to its rich source of multiple secondary metabolites such as ajmaline, ajmalicine, reserpine, and serpentine. To meet the constant demands for the key secondary metabolite (reserpine) by majority of the pharmaceutical industries, the present study assessed the effects of direct and indirect regeneration system on amelioration of reserpine accumulation in shoots of R. serpentina. In vitro multiple shoot cultures were established using shoot tip explants. Best results for shoot initiation, multiplication, and biomass production were obtained in case of Murashige and Skoog medium, supplemented with 1 mg/l N 6 -benzyladenine. The multiple shoots were then sub-cultured on cytokinin-auxin combination media for further proliferation. Highest shoot and leaf multiplication rates and the most enhanced biomass were obtained in case of 1-1.5 mg/l Kinetin + 0.2 mg/l α-naphthalene acetic acid (NAA). Callus induction and its subsequent proliferation was obtained using 1.5 mg/l 2,4-dichlorophenoxyacetic acid. The best indirect shoot regeneration with highest shoot and leaf proliferation from calli was observed in case of 1 mg/l thidiazuron + 0.2 mg/l NAA. Reserpine content estimation via HPTLC from in vitro shoots (direct regeneration) and calli (indirect regeneration) were recorded to undergo an almost three-fold and two-fold increment (respectively) in comparison to that of the mother plant. Thus, in vitro direct regeneration system proved to be more effective and efficient in ameliorating the reserpine content.

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Embryogenic cell suspension culture induction and plantlet regeneration ofRauvolfia serpentina(L.) Benth.: influence of different plant growth regulator concentrations and combinations

Cited by 8 publications

References 0 publications

In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

Cell Suspension Culture and In Vitro Screening for Drought Tolerance in Soybean Using Poly-Ethylene Glycol

Ameliorated reserpine production via in vitro direct and indirect regeneration system in Rauvolfia serpentina (L.) Benth. ex Kurz.

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