Embryological origin of interstitial cells of Cajal

Young, Heather M.

doi:10.1002/(sici)1097-0029(19991201)47:5<303::aid-jemt1>3.0.co;2-t

Cited by 76 publications

(50 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together these results suggest that the mutation causing ICC-IM loss in these animals also leads to abnormalities of the smooth muscle. This is perhaps not surprising given the plethora of pathological abnormalities in these mutant animals (2,22,27,30,33) and the fact that ICCs and smooth muscle cells share the same precursor cells (36). It is uncertain whether the abnormalities are due to a direct effect of the mutation on the smooth muscle or a consequence of a developmental abnormality; that is, perhaps ICCs are required for normal maturation and differentiation of smooth muscle cells.…”

Section: Discussionmentioning

confidence: 99%

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/W^vmutant mice

Bautista-Cruz

Paterson

2011

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/W^vmutant mice

Bautista-Cruz

Paterson

2011

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

show abstract

“…However, it seems reasonable to assume important differences between the two cell types, as their proportions in the whole cell population are significantly dissimilar [101]. Differences can be also found when looking at the origins of these cells: CD34 positive fibroblasts might have a bloodborne precursor [104] that crosses endothelia, whereas ICC and Cajal-like cells are more likely to arise from tissue-resident mesenchymal progenitor cells [105].…”

Section: T-icc: An Immune Surveillance Role?mentioning

confidence: 99%

Novel type of interstitial cell (Cajal-like) in human fallopian tube

et al. 2005

View full text Add to dashboard Cite

We describe here ‐ presumably for the first time‐a Cajal‐like type of tubal interstitial cells (t‐ICC), resembling the archetypal enteric ICC. t‐ICC were demonstrated in situ and in vitro on fresh preparations (tissue cryosections and primary cell cultures) using methylene‐blue, crystal‐violet, Janus‐Green B or Mito Tracker‐Green FM Probe vital stainings. Also, t‐ICC were identified in fixed specimens by light microscopy (methylene‐blue, Giemsa, trichrome stainings, Gomori silver‐impregnation) or transmission electron microscopy (TEM). The positive diagnosis of t‐ICC was strengthened by immunohistochemistry (IHC; CD117/c‐kit+ and other 14 antigens) and immunofluorescence (IF; CD117/c‐kit+ and other 7 antigens). The spatial density of t‐ICC (ampullar‐segment cryosections) was 100–150 cells/mm2. Non‐conventional light microscopy (NCLM) of Epon semithin‐sections revealed a network‐like distribution of t‐ICC in lammina propria and smooth muscle meshwork. t‐ICC appeared located beneath of epithelium, in a 10–15μ thick ‘belt’, where 18±2% of cells were t‐ICC. In the whole lamina propria, t‐ICC were about 9%, and in muscularis ∼7%. In toto, t‐ICC represent ∼8% of subepithelial cells, as counted by NCLM. In vitro, t‐ICC were 9.9±0.9% of total cell population. TEM showed that the diagnostic ‘gold standard’ (Huizinga et al., 1997) is fulfilled by ‘our’ t‐ICC. However, we suggest a ‘platinum standard’, adding a new defining criterion ‐ characteristic cytoplasmic processes (number: 1–5; length: tens of μm; thickness: ±0.5μ; aspect: moniliform; braching: dichotomous; organization: network, labyrinthic‐system). Quantitatively, the ultrastructural architecture of t‐ICC is: nucleus, 23.6±3.2% of cell volume, with heterochromatin 49.1±3.8%; mitochondria, 4.8±1.7%; rough and smooth endoplasmic‐reticulum (1.1±0.6%, 1.0±0.2%, respectively); caveolae, 3.4±0.5%. We found more caveolae on the surface of cell processes versus cell body, as confirmed by IF for caveolins. Occasionally, the so‐called ‘Ca2+‐release units’ (subplasmalemmal close associations of caveolae+endoplasmic reticulum±mitochondria) were detected in the dilations of cell processes. Electrophysiological single unit recordings of t‐ICC in primary cultures indicated sustained spontaneous electrical activity (amplitude of field potentials: 57.26±6.56mV). Besides the CD117/c‐kit marker, t‐ICC expressed variously CD34, caveolins 1&2, α‐SMA, S‐100, vimentin, nestin, desmin, NK‐1. t‐ICC were negative for: CD68, CD1a, CD62P, NSE, GFAP, chromogranin‐A, PGP9.5, but IHC showed the possible existence of (neuro)endocrine cells in tubal interstitium. We call them ‘JF cells’. In conclusion, the identification of t‐ICC might open the door for understanding some tubal functions, e.g. pace‐making/peristaltism, secretion (auto‐, juxta‐ and/or paracrine), regulation of neurotransmission (nitrergic/purinergic) and intercellular signaling, via the very long processes. Furthermore, t‐ICC might even be uncommitted bipotential progenitor cells.

show abstract

“…2 Although the embryologic origin is unclear, it has been shown that interstitial cells originate from the mesenchymal cells. 3 The finding that Cajal cells express c-kit (CD117), a tyrosine kinase, is a considerable development. The c-kit is a membrane receptor protein, a growth factor and a protooncogene.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and electron microscopic examination of Cajal cells in ureteropelvic junction obstruction

Eken

Erdogan

Kuyucu

et al. 2013

CUAJ

View full text Add to dashboard Cite

E311Cite as: Can Urol Assoc J 2013;7(5-6):e311-6. http://dx.doi.org/10.5489/cuaj.11293 Published online May 13, 2013 (early released September 10, 2012). AbstractObjective: We examine the ultrastructural configurations of Cajal cells by electron microscopy, as well as the quantitative changes occurring in Cajal cells by light microscopy. Methods: In total, 35 patients with ureteropelvic junction (UPJ) obstruction and 7 patients without obstruction were compared immunohistochemically with c-kit (CD117) to quantify the number of cells. On electron microscopic examination, 7 patients with UPJ obstruction and 3 patients without obstruction were compared to evaluate the changes which occurred in the ultrastructural configuration of the Cajal cells. Results: On light microscopic examination, it was determined that the Cajal cells, which demonstrate c-kit (CD117) immunoreactive character, were located near the circular muscle layer and parallel to the muscle cells. The number of Cajal cells in the control group was significantly increased compared to the number of cells in patients with UPJ obstruction (p < 0.001). On electron microscopic examination, the number of interstitial cells was also higher in the control group. A decrease in the number of the caveolae in these cells was seen in the group with UPJ obstruction compared to the control group. Conclusion: In UPJ obstruction, a decrease in the number of Cajal cells, as well as the changes in the morphologic structure of the Cajal cells, indicates that these cells have a role in the pacemaker system and are associated with ureteral peristalsis.

show abstract

Embryological origin of interstitial cells of Cajal

Cited by 76 publications

References 46 publications

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/W^vmutant mice

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/W^vmutant mice

Novel type of interstitial cell (Cajal-like) in human fallopian tube

Immunohistochemical and electron microscopic examination of Cajal cells in ureteropelvic junction obstruction

Contact Info

Product

Resources

About

Embryological origin of interstitial cells of Cajal

Cited by 76 publications

References 46 publications

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/Wvmutant mice

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/Wvmutant mice

Novel type of interstitial cell (Cajal-like) in human fallopian tube

Immunohistochemical and electron microscopic examination of Cajal cells in ureteropelvic junction obstruction

Contact Info

Product

Resources

About

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/W^vmutant mice

Evidence for altered circular smooth muscle cell function in lower esophageal sphincter ofW/W^vmutant mice