Embryonic Stem Cell-Like Population in Dupuytren’s Disease Expresses Components of the Renin-Angiotensin System

On, Nicholas; Koh, Sabrina P.; Brasch, Helen D.; Dunne, Jonathan C.; Armstrong, J. R.; Tan, Swee T.; Itinteang, Tinte

doi:10.1097/gox.0000000000001422

Cited by 14 publications

(17 citation statements)

References 31 publications

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“…IF IHC staining was performed to determine the localization of each cathepsin in relation to the ESC-like population within DD tissue 7 that expresses the components of the RAS. 8 Cathepsin B (Fig. 1 A, D, red) and cathepsin D (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…DD tissue samples from 10 male patients aged 66–78 (mean, 69.3) years including 6 patients used in our previous studies 7 , 8 were sourced from the Gillies McIndoe Research Institute Tissue Bank and used for this study that was approved by the Central Regional Health and Disability Ethics Committee (ref. no.…”

Section: Methodsmentioning

confidence: 99%

“… 7 This primitive population expresses components of the renin-angiotensin system (RAS), namely pro(renin) receptor, angiotensin converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1) and angiotensin II receptor 2 (ATIIR2). 8 These findings suggest that dysfunction of the ESC-like population within the microvessels of DD may give rise to an intermediate MSC population, which in turn gives rise to the abundant downstream myofibroblasts—the dominant cell type within DD. 9 A putative role for the RAS in regulating this ESC-like population has been speculated, reminiscent of the findings in infantile hemangioma.…”

Section: Introductionmentioning

confidence: 91%

“…In this study, we investigated the expression of cathepsins B, D, and G, and their localization in relation to the ESC-like population within DD 7 that expresses components of the RAS, 8 using immunohistochemical (IHC) staining, Western blotting (WB), and Nanostring mRNA analysis. We also investigated the function of these enzymes using enzyme activity assays.…”

Section: Introductionmentioning

confidence: 99%

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Expression and Localization of Cathepsins B, D, and G in Dupuytren’s Disease

Tan

Brasch

Schaijik

et al. 2018

Plastic and Reconstructive Surgery - Global Open

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression and Localization of Cathepsins B, D, and G in Dupuytren’s Disease

Tan

Brasch

Schaijik

et al. 2018

Plastic and Reconstructive Surgery - Global Open

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“…The role of adipose tissue in angiogenesis is known[ 19 ], and the presence of markers of angiogenesis in Dupuytren’s contracture palmar aponeurosis was proved[ 20 ]. The composition of the luminal lining of microvessels revealed potential precursors of myofibroblasts[ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of palmar subcutaneous tissue vascularization in patients with Dupuytren’s contracture

Щудло¹,

Варсегова²,

Ступина³

et al. 2018

WJO

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AIMTo investigate the structural and functional characteristics of palmar hypodermal tissue vascularization in Dupuytren’s contracture patients of different age groups.METHODSEighty-seven Dupuytren’s contracture patients underwent partial fasciectomy. Twenty-two of them were less than 55 years old (Y-group, n = 22); the others were 55 and older (O-group, n = 65). In surgically excised representative tissue samples, a histomorphometric analysis of the perforating arteries of the palmar aponeurosis and stereologic analysis of hypodermis vascularity were performed. The method of laser flowmetry estimated the microcirculation of the skin of the palm.RESULTSFrequency of cases with rapid development of contracture (less than 5 years) was 13.6% in the Y-group and 40% in the O-group, P < 0.05. The external and luminal diameters of perforating arteries in palmar fascia were decreased more severely in Y. The thickness of intima increased three times compared with healthy control, and the intima/media relation also increased, especially in O. Increased numerical and volumetric micro-vessel densities in hypodermis, percentage of large vessels (more than 12 μm in diameter), and percentage of vessels with signs of periadventitial inflammatory infiltration were noted in Y. The percentage of vessels with adventitial fibrosis was greater in O than in Y. Base capillary flow in Y was increased compared to healthy control subjects and to O, and peak capillary flow was increased in comparison with control.CONCLUSIONCompared to the O-group, Y-group patients exhibited more severe constrictive remodeling of palmar fascia perforating arteries supplying hypodermis but more expressed compensatory changes of its capillarization.

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